Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction
Zn2Cys6 transcription factors are unique to fungi and are involved in different regulatory functions. In this study, we have identified the Penicillium digitatum PdMut3 gene, which encodes a putative Zn (II) 2Cys6 DNA-binding protein. Elimination of PdMut3 in Pd1 strain caused increased virulence du...
| Main Authors: | , |
|---|---|
| Format: | article |
| Language: | Inglés |
| Published: |
MDPI
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7765 https://www.mdpi.com/2309-608X/7/10/828 |
| _version_ | 1855032666342031360 |
|---|---|
| author | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_browse | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_facet | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_sort | De-Ramón-Carbonell, Marta |
| collection | ReDivia |
| description | Zn2Cys6 transcription factors are unique to fungi and are involved in different regulatory functions. In this study, we have identified the Penicillium digitatum PdMut3 gene, which encodes a putative Zn (II) 2Cys6 DNA-binding protein. Elimination of PdMut3 in Pd1 strain caused increased virulence during citrus infection. The transcription of the PdMut3 gene showed a higher expression rate during fungal growth and less transcription during fruit infection. Furthermore, the deletion of the gene in the wild-type isolate of P. digitatum did not produce any modification of the sensitivity to different fungicides, indicating that the gene is not associated with resistance to fungicides. In contrast, PdMut3 null mutants showed a reduction in growth in minimal media, which was associated
with severe alterations in conidiophore development and morphological alterations of the hyphae. Mutants showed greater sensitivity to compounds that interfere with the cell wall and an invasive growth block. Thus, PdMut3 might have an indirect role in fungi virulence through metabolism and peroxisomes development. |
| format | article |
| id | ReDivia7765 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia77652025-04-25T14:48:34Z Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma Postharvest Peroxisomes J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q01 Food science and technology Citrus Resistance to fungicides Penicillium digitatum Transcription factors Virulence Zn2Cys6 transcription factors are unique to fungi and are involved in different regulatory functions. In this study, we have identified the Penicillium digitatum PdMut3 gene, which encodes a putative Zn (II) 2Cys6 DNA-binding protein. Elimination of PdMut3 in Pd1 strain caused increased virulence during citrus infection. The transcription of the PdMut3 gene showed a higher expression rate during fungal growth and less transcription during fruit infection. Furthermore, the deletion of the gene in the wild-type isolate of P. digitatum did not produce any modification of the sensitivity to different fungicides, indicating that the gene is not associated with resistance to fungicides. In contrast, PdMut3 null mutants showed a reduction in growth in minimal media, which was associated with severe alterations in conidiophore development and morphological alterations of the hyphae. Mutants showed greater sensitivity to compounds that interfere with the cell wall and an invasive growth block. Thus, PdMut3 might have an indirect role in fungi virulence through metabolism and peroxisomes development. 2021-11-23T14:54:29Z 2021-11-23T14:54:29Z 2021 article publishedVersion de-Ramón-Carbonell, M. & Sánchez-Torres, P. (2021). Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction. Journal of Fungi, 7(10), 828. 2309-608X http://hdl.handle.net/20.500.11939/7765 10.3390/jof7100828 https://www.mdpi.com/2309-608X/7/10/828 en info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2008-04828-C03 info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2011-30519-C03-02/ES/CARACTERIZACION DE LOS MECANISMOS DE VIRULENCIA Y DEFENSA EN LA INTERACCION HONGO-FRUTO COMO HERRAMIENTA PARA EL CONTROL DE PENICILLIUM PATOGENOS DE CITRICOS Y DE MANZANAS This work was supported by funds of AGL2008-04828-C03 and AGL2011-30519-C03-02 projects from the Ministry of Science and Innovation (Spain). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Postharvest Peroxisomes J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q01 Food science and technology Citrus Resistance to fungicides Penicillium digitatum Transcription factors Virulence De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title | Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title_full | Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title_fullStr | Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title_full_unstemmed | Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title_short | Unveiling the Role Displayed by Penicillium digitatum PdMut3 Transcription Factor in Pathogen–Fruit Interaction |
| title_sort | unveiling the role displayed by penicillium digitatum pdmut3 transcription factor in pathogen fruit interaction |
| topic | Postharvest Peroxisomes J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q01 Food science and technology Citrus Resistance to fungicides Penicillium digitatum Transcription factors Virulence |
| url | http://hdl.handle.net/20.500.11939/7765 https://www.mdpi.com/2309-608X/7/10/828 |
| work_keys_str_mv | AT deramoncarbonellmarta unveilingtheroledisplayedbypenicilliumdigitatumpdmut3transcriptionfactorinpathogenfruitinteraction AT sancheztorrespaloma unveilingtheroledisplayedbypenicilliumdigitatumpdmut3transcriptionfactorinpathogenfruitinteraction |