Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress
This work compares the tolerance to long-term anoxia conditions (35 days) of five new citrus ‘King’ mandarin (Citrus nobilis L. Lour) Poncirus trifoliata ((L.) Raf.) hybrids (named 0501XX) and Carrizo citrange (CC, Citrus sinensis (L.) Osb. Poncirus trifoliata (L.) Raf.), the widely used citrus...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7742 https://www.mdpi.com/2311-7524/7/10/388 |
| _version_ | 1855492350771462144 |
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| author | Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Forner-Giner, María A. |
| author_browse | Forner-Giner, María A. Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo |
| author_facet | Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Forner-Giner, María A. |
| author_sort | Martínez-Cuenca, Mary-Rus |
| collection | ReDivia |
| description | This work compares the tolerance to long-term anoxia conditions (35 days) of five new citrus ‘King’ mandarin (Citrus nobilis L. Lour) Poncirus trifoliata ((L.) Raf.) hybrids (named 0501XX) and Carrizo citrange (CC, Citrus sinensis (L.) Osb. Poncirus trifoliata (L.) Raf.), the widely used citrus rootstock in Spain. Growth parameters, chlorophyll concentration, gas exchange and fluorescence
parameters, water relations in leaves, abscisic acid (ABA) concentration, and PIP1 and PIP2 gene expressions were assessed. With a waterlogging treatment, the root system biomass of most hybrids went down, and the chlorophyll a and b concentrations substantially dropped. The net CO2 assimilation rates (An) and stomatal conductance (gs) lowered significantly due to flooding, and the transpiration rate (E) closely paralleled the changes in gs. The leaf water and osmotic potentials significantly increased in most 0501 hybrids. As a trend, flooding stress lowered the ABA concentration in roots from most hybrids, but increased in the leaves of CC, 05019 and 050110. Under the control treatment (Ct) conditions, most 0501 hybrids showed higher PIP1 and PIP2 expressions than the control rootstock CC, but were impaired due to the flooding conditions in 05019 and 050110. From this study, we conclude that 0501 genotypes develop some adaptive responses in plants against flooding stress such as (1) stomata closure to prevent water loss likely mediated by ABA levels, and (2) enhanced water and osmotic potentials and the downregulation of those genes regulating aquaporin channels to maintain water relations in plants. Although these traits seemed especially relevant in hybrids 050110 and 050125, further experiments must be done to determine their behavior under field conditions, particularly their influence on commercial varieties and their suitability as flooding-tolerant hybrids for replacing CC, one of the main genotypes that is widely used as a citrus rootstock in Spain, under these conditions |
| format | Artículo |
| id | ReDivia7742 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia77422025-04-25T14:48:32Z Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Forner-Giner, María A. Chlorophyll concentration Citrus hybrids Gas exchange parameters PIPs F30 Plant genetics and breeding F60 Plant physiology and biochemistry F06 Irrigation F61 Plant physiology - Nutrition Abscisic acid Fluorescence Osmotic potential Water potential Waterlogging This work compares the tolerance to long-term anoxia conditions (35 days) of five new citrus ‘King’ mandarin (Citrus nobilis L. Lour) Poncirus trifoliata ((L.) Raf.) hybrids (named 0501XX) and Carrizo citrange (CC, Citrus sinensis (L.) Osb. Poncirus trifoliata (L.) Raf.), the widely used citrus rootstock in Spain. Growth parameters, chlorophyll concentration, gas exchange and fluorescence parameters, water relations in leaves, abscisic acid (ABA) concentration, and PIP1 and PIP2 gene expressions were assessed. With a waterlogging treatment, the root system biomass of most hybrids went down, and the chlorophyll a and b concentrations substantially dropped. The net CO2 assimilation rates (An) and stomatal conductance (gs) lowered significantly due to flooding, and the transpiration rate (E) closely paralleled the changes in gs. The leaf water and osmotic potentials significantly increased in most 0501 hybrids. As a trend, flooding stress lowered the ABA concentration in roots from most hybrids, but increased in the leaves of CC, 05019 and 050110. Under the control treatment (Ct) conditions, most 0501 hybrids showed higher PIP1 and PIP2 expressions than the control rootstock CC, but were impaired due to the flooding conditions in 05019 and 050110. From this study, we conclude that 0501 genotypes develop some adaptive responses in plants against flooding stress such as (1) stomata closure to prevent water loss likely mediated by ABA levels, and (2) enhanced water and osmotic potentials and the downregulation of those genes regulating aquaporin channels to maintain water relations in plants. Although these traits seemed especially relevant in hybrids 050110 and 050125, further experiments must be done to determine their behavior under field conditions, particularly their influence on commercial varieties and their suitability as flooding-tolerant hybrids for replacing CC, one of the main genotypes that is widely used as a citrus rootstock in Spain, under these conditions 2021-11-09T15:05:59Z 2021-11-09T15:05:59Z 2021 article publishedVersion Martínez-Cuenca, M. R., Primo-Capella, A. & Forner-Giner, M.Á. (2021) Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress. Horticulturae, 7(10), 388. 2311-7524 http://hdl.handle.net/20.500.11939/7742 10.3390/horticulturae7100388 https://www.mdpi.com/2311-7524/7/10/388 en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018–098379-R-I00//OBTENCION, SELECCION, CARACTERIZACION Y EVALUACION DE NUEVOS PATRONES DE CITRICOS info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51913 This study was cofunded by the Ministerio de Ciencia e Innovación (RTI2018-098379-R-I00) and by the European Union through the European Regional Development Fund (ERDF) of the Generalitat Valenciana 2014–2020 (IVIA-51913). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Chlorophyll concentration Citrus hybrids Gas exchange parameters PIPs F30 Plant genetics and breeding F60 Plant physiology and biochemistry F06 Irrigation F61 Plant physiology - Nutrition Abscisic acid Fluorescence Osmotic potential Water potential Waterlogging Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Forner-Giner, María A. Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title | Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title_full | Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title_fullStr | Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title_full_unstemmed | Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title_short | Screening of ‘King’ Mandarin Hybrids as Tolerant Citrus Rootstocks to Flooding Stress |
| title_sort | screening of king mandarin hybrids as tolerant citrus rootstocks to flooding stress |
| topic | Chlorophyll concentration Citrus hybrids Gas exchange parameters PIPs F30 Plant genetics and breeding F60 Plant physiology and biochemistry F06 Irrigation F61 Plant physiology - Nutrition Abscisic acid Fluorescence Osmotic potential Water potential Waterlogging |
| url | http://hdl.handle.net/20.500.11939/7742 https://www.mdpi.com/2311-7524/7/10/388 |
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