Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnos...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/7737 https://www.mdpi.com/1999-4915/13/11/2233 |
| _version_ | 1855492349813063680 |
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| author | Ruiz-García, Ana B. Canales, Celia Morán, Félix Ruiz-Torres, Manuel Herrera-Mármol, Magdalena Olmos, Antonio |
| author_browse | Canales, Celia Herrera-Mármol, Magdalena Morán, Félix Olmos, Antonio Ruiz-García, Ana B. Ruiz-Torres, Manuel |
| author_facet | Ruiz-García, Ana B. Canales, Celia Morán, Félix Ruiz-Torres, Manuel Herrera-Mármol, Magdalena Olmos, Antonio |
| author_sort | Ruiz-García, Ana B. |
| collection | ReDivia |
| description | The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnostic purposes. In this study, we report for the first time in Spanish orchards the presence of olive leaf yellowing-associated virus (OLYaV), for which the second full coding sequence has been determined. This virus has also been detected in a putative vector, the psyllid Euphyllura olivina. In addition, the presence in Spain of Olea europaea geminivirus (OEGV), recently reported in Italy, has been confirmed, and the full-length sequence of two isolates was obtained by HTS and Sanger sequencing. These results, as well as the detection of other viral sequences related to olive latent virus 3 (OLV-3) and olive viral satellite RNA, raises questions on the biological significance of the findings, about the requirement of standardization on the interpretation of HTS results, and the necessity of additional tests to confirm the relevance of the HTS detection of viral sequences |
| format | Artículo |
| id | ReDivia7737 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia77372025-04-25T14:48:31Z Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties Ruiz-García, Ana B. Canales, Celia Morán, Félix Ruiz-Torres, Manuel Herrera-Mármol, Magdalena Olmos, Antonio HTS NGS OLYaV OEGV OLV-3 Virome olive latent virus 3 H20 Plant diseases Olives Olea europaea olive leaf yellowing-associated virus Euphyllura olivina Olea europaea geminivirus The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnostic purposes. In this study, we report for the first time in Spanish orchards the presence of olive leaf yellowing-associated virus (OLYaV), for which the second full coding sequence has been determined. This virus has also been detected in a putative vector, the psyllid Euphyllura olivina. In addition, the presence in Spain of Olea europaea geminivirus (OEGV), recently reported in Italy, has been confirmed, and the full-length sequence of two isolates was obtained by HTS and Sanger sequencing. These results, as well as the detection of other viral sequences related to olive latent virus 3 (OLV-3) and olive viral satellite RNA, raises questions on the biological significance of the findings, about the requirement of standardization on the interpretation of HTS results, and the necessity of additional tests to confirm the relevance of the HTS detection of viral sequences 2021-11-08T11:42:58Z 2021-11-08T11:42:58Z 2021 article publishedVersion Ruiz-García, A.B.; Canales, C.; Morán, F.; Ruiz-Torres, M.; Herrera-Mármol, M.; Olmos, A. Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties. Viruses 2021, 13, 2233. 1999-4915 http://hdl.handle.net/20.500.11939/7737 10.3390/v13112233 https://www.mdpi.com/1999-4915/13/11/2233 en Info:eu-repo/grantAgreement/EC/H2020/734736 European Union’s Horizon 2020 research and innovation programme, under the Marie Skłodowska-Curie grant agreement No. 734736. openAccess MDPI electronico |
| spellingShingle | HTS NGS OLYaV OEGV OLV-3 Virome olive latent virus 3 H20 Plant diseases Olives Olea europaea olive leaf yellowing-associated virus Euphyllura olivina Olea europaea geminivirus Ruiz-García, Ana B. Canales, Celia Morán, Félix Ruiz-Torres, Manuel Herrera-Mármol, Magdalena Olmos, Antonio Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title | Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title_full | Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title_fullStr | Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title_full_unstemmed | Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title_short | Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties |
| title_sort | characterization of spanish olive virome by high throughput sequencing opens new insights and uncertainties |
| topic | HTS NGS OLYaV OEGV OLV-3 Virome olive latent virus 3 H20 Plant diseases Olives Olea europaea olive leaf yellowing-associated virus Euphyllura olivina Olea europaea geminivirus |
| url | http://hdl.handle.net/20.500.11939/7737 https://www.mdpi.com/1999-4915/13/11/2233 |
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