First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequ...
| Autores principales: | , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
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MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7707 https://www.mdpi.com/2223-7747/10/11/2293 |
| _version_ | 1855032655577350144 |
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| author | Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. |
| author_browse | Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. |
| author_facet | Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. |
| author_sort | Canales, Celia |
| collection | ReDivia |
| description | Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date. |
| format | publishedVersion |
| id | ReDivia7707 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia77072025-04-25T14:48:29Z First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. ASGV CTLV ACLSV AHVd loquat HTS NGS H01 Protection of plants - General aspects H20 Plant diseases H10 Pests of plants Loquats Apple stem grooving virus citrus tatter leaf virus apple chlorotic leaf spot virus apple hammerhead viroid Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date. 2021-10-28T15:25:44Z 2021-10-28T15:25:44Z 2021 publishedVersion Canales, C., Morán, F., Olmos, A. & Ruiz-García, A. B. (2021). First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus and Apple Hammerhead Viroid in Loquat in Spain. Plants 2021, 10, 2293. 2223-7747 http://hdl.handle.net/20.500.11939/7707 10.3390/plants10112293 https://www.mdpi.com/2223-7747/10/11/2293 en Info:eu-repo/grantAgreement/EC/H2020/734736 European Union’s Horizon 2020 research and innovation programme, under the Marie Skłodowska-Curie grant agreement No 734736. openAccess MDPI electronico |
| spellingShingle | ASGV CTLV ACLSV AHVd loquat HTS NGS H01 Protection of plants - General aspects H20 Plant diseases H10 Pests of plants Loquats Apple stem grooving virus citrus tatter leaf virus apple chlorotic leaf spot virus apple hammerhead viroid Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title | First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title_full | First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title_fullStr | First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title_full_unstemmed | First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title_short | First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |
| title_sort | first detection and molecular characterization of apple stem grooving virus apple chlorotic leaf spot virus and apple hammerhead viroid in loquat in spain |
| topic | ASGV CTLV ACLSV AHVd loquat HTS NGS H01 Protection of plants - General aspects H20 Plant diseases H10 Pests of plants Loquats Apple stem grooving virus citrus tatter leaf virus apple chlorotic leaf spot virus apple hammerhead viroid |
| url | http://hdl.handle.net/20.500.11939/7707 https://www.mdpi.com/2223-7747/10/11/2293 |
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