Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo
Previous studies on Atalantia citroides, a citrus relative that appeared to be immune to viroid infection, revealed the existence of a new viroid, which was designated tentatively as Citrus viroid-V (CVd-V) and has been proposed as a new member of the genus Apscaviroid within the family Pospiviroida...
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| Format: | conferenceObject |
| Language: | Inglés |
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IOCV
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/7704 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/17.%20Serra.pdf |
| _version_ | 1855032654996439040 |
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| author | Serra, Pedro Pina, José A. Durán-Vila, Núria |
| author2 | Hilf, M. E. |
| author_browse | Durán-Vila, Núria Hilf, M. E. Pina, José A. Serra, Pedro |
| author_facet | Hilf, M. E. Serra, Pedro Pina, José A. Durán-Vila, Núria |
| author_sort | Serra, Pedro |
| collection | ReDivia |
| description | Previous studies on Atalantia citroides, a citrus relative that appeared to be immune to viroid infection, revealed the existence of a new viroid, which was designated tentatively as Citrus viroid-V (CVd-V) and has been proposed as a new member of the genus Apscaviroid within the family Pospiviroidae. Biological indexing of a Seminole tangelo tree on the Etrog citron indicator followed by sPAGE analysis revealed the presence of a viroid that has been characterized as a new variant of CVd-V. The variant has 97.6% sequence identity with the reference sequence of CVd-V and shows three characteristics: (I) two compensatory changes that modify an A-U base pair between the upper and lower “strands” into a G-C base pair, (II) a U→A change located in a loop that does not seem to disturb the viroid secondary structure, and (III) two sets of changes located in the upper and lower strands that result in a rearrangement of the base pairing between the upper and lower strands. Infectivity studies performed with an artificial mutant revealed the existence of structural constraints in the region in which major differences between CVd-V and the Seminole tangelo variant were identified. |
| format | conferenceObject |
| id | ReDivia7704 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77042025-04-25T14:53:18Z Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo Serra, Pedro Pina, José A. Durán-Vila, Núria Hilf, M. E. Citrus viroids Apscaviroid Structural motifs H20 Plant diseases Previous studies on Atalantia citroides, a citrus relative that appeared to be immune to viroid infection, revealed the existence of a new viroid, which was designated tentatively as Citrus viroid-V (CVd-V) and has been proposed as a new member of the genus Apscaviroid within the family Pospiviroidae. Biological indexing of a Seminole tangelo tree on the Etrog citron indicator followed by sPAGE analysis revealed the presence of a viroid that has been characterized as a new variant of CVd-V. The variant has 97.6% sequence identity with the reference sequence of CVd-V and shows three characteristics: (I) two compensatory changes that modify an A-U base pair between the upper and lower “strands” into a G-C base pair, (II) a U→A change located in a loop that does not seem to disturb the viroid secondary structure, and (III) two sets of changes located in the upper and lower strands that result in a rearrangement of the base pairing between the upper and lower strands. Infectivity studies performed with an artificial mutant revealed the existence of structural constraints in the region in which major differences between CVd-V and the Seminole tangelo variant were identified. 2021-10-27T14:43:01Z 2021-10-27T14:43:01Z 2010 conferenceObject Serra, P., Pina, J. A. & Durán-Vila, N. (2010). Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo . Proceedings of the Seventeenth Conference of the International Organization of Citrus Virologists, 150-157. Riverside, California. 2313-5123 0074-7203 (e-ISSN) http://hdl.handle.net/20.500.11939/7704 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/17.%20Serra.pdf en 2007-10-22 Seventeenth Conference of the International Organization of Citrus Virologists Adana, Turkey This research was supported by grant AGL2005-01468 from the Ministerio de Educación y Ciencia. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Citrus viroids Apscaviroid Structural motifs H20 Plant diseases Serra, Pedro Pina, José A. Durán-Vila, Núria Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title | Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title_full | Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title_fullStr | Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title_full_unstemmed | Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title_short | Identification and Characterization of a Variant of Citrus viroid V (CVd-V) in Seminole Tangelo |
| title_sort | identification and characterization of a variant of citrus viroid v cvd v in seminole tangelo |
| topic | Citrus viroids Apscaviroid Structural motifs H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/7704 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/17.%20Serra.pdf |
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