Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides
Atalantia citroides propagated on rough lemon rootstock was graft-inoculated in the scion with an artificial mixture of Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus viroid III (CVd-III) and Citrus viroid IV (CVd-IV). Three years later, bioassays i...
| Autores principales: | , , , , , , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7702 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV%2016.pdf |
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| author | Barbosa, C. J. Serra, Pedro Pina, José A. Navarro, Luis Daros, José A. Flores, Ricardo Durán-Vila, Núria |
| author2 | Hilf, M. E. |
| author_browse | Barbosa, C. J. Daros, José A. Durán-Vila, Núria Flores, Ricardo Hilf, M. E. Navarro, Luis Pina, José A. Serra, Pedro |
| author_facet | Hilf, M. E. Barbosa, C. J. Serra, Pedro Pina, José A. Navarro, Luis Daros, José A. Flores, Ricardo Durán-Vila, Núria |
| author_sort | Barbosa, C. J. |
| collection | ReDivia |
| description | Atalantia citroides propagated on rough lemon rootstock was graft-inoculated in the scion with an artificial mixture of Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus viroid III (CVd-III) and Citrus viroid IV
(CVd-IV). Three years later, bioassays in Etrog citron and slot-blot hybridization with viroid specific probes showed that all the inoculated viroids were detected in the rootstock but none in the scion. However, sequential polyacrylamide gel electrophoresis (sPAGE) showed the presence in the A. citroides scion of a viroid-like RNA with an electrophoretic mobility between HSVd and CVd-III. Further analysis by denaturing PAGE of purified preparations thereof revealed two bands with the mobility expected for the circular and linear forms of viroids. Infectivity of the viroid-like RNA was demonstrated by graft transmission to Etrog citron as well as by slash inoculation with purified preparations. Sequencing of three partial-length cDNA clones of this new RNA species, obtained by retrotranscription and PCR amplification (RT-PCR) approach that does not require prior sequence knowledge showed that the sequences corresponded to the central conserved region characteristic of members of the genus Apscaviroid. Complete molecular characterization is in progress. |
| format | Objeto de conferencia |
| id | ReDivia7702 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77022025-04-25T14:53:09Z Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides Barbosa, C. J. Serra, Pedro Pina, José A. Navarro, Luis Daros, José A. Flores, Ricardo Durán-Vila, Núria Hilf, M. E. Apscaviroid Graft transmission Citrus viroids H20 Plant diseases U30 Research methods Atalantia citroides propagated on rough lemon rootstock was graft-inoculated in the scion with an artificial mixture of Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus viroid III (CVd-III) and Citrus viroid IV (CVd-IV). Three years later, bioassays in Etrog citron and slot-blot hybridization with viroid specific probes showed that all the inoculated viroids were detected in the rootstock but none in the scion. However, sequential polyacrylamide gel electrophoresis (sPAGE) showed the presence in the A. citroides scion of a viroid-like RNA with an electrophoretic mobility between HSVd and CVd-III. Further analysis by denaturing PAGE of purified preparations thereof revealed two bands with the mobility expected for the circular and linear forms of viroids. Infectivity of the viroid-like RNA was demonstrated by graft transmission to Etrog citron as well as by slash inoculation with purified preparations. Sequencing of three partial-length cDNA clones of this new RNA species, obtained by retrotranscription and PCR amplification (RT-PCR) approach that does not require prior sequence knowledge showed that the sequences corresponded to the central conserved region characteristic of members of the genus Apscaviroid. Complete molecular characterization is in progress. 2021-10-27T10:47:05Z 2021-10-27T10:47:05Z 2005 conferenceObject Barbosa, C. J., Serra, P., Pina, J. A., Navarro, L., Daros, J. A., Flores, R. et al. (2005). Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides. Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 264-271. 2313-5123 0074-7203 (e-ISSN) http://hdl.handle.net/20.500.11939/7702 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV%2016.pdf en 2004-11-07 Sixteenth Conference of the International Organization of Citrus Virologists Monterrey, Mexico Work in the N. Duran-Vila laboratory was supported by grants RTA01-119 from the Ministerio de Ciencia y Tecnología (MCyT) and CV04B239 from the Generalitat Valenciana (GV) and work in the R. Flores and J. A. Daròs laboratory by grants from the MCyT (BMC2002- 03694) and the GV (Grupos 03/064). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Apscaviroid Graft transmission Citrus viroids H20 Plant diseases U30 Research methods Barbosa, C. J. Serra, Pedro Pina, José A. Navarro, Luis Daros, José A. Flores, Ricardo Durán-Vila, Núria Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title | Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title_full | Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title_fullStr | Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title_full_unstemmed | Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title_short | Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides |
| title_sort | identification and preliminary characterization of a viroid like rna in atalantia citroides |
| topic | Apscaviroid Graft transmission Citrus viroids H20 Plant diseases U30 Research methods |
| url | http://hdl.handle.net/20.500.11939/7702 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV%2016.pdf |
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