Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain
Sixty citrus tristeza virus (CTV) isolates representative of most Spanish citrus areas were collected and initially inoculated on Mexican lime and Duncan grapefruit. Ten of them were selected on the basis of origin, host and symptoms on those indicator plants. Selected isolates were separated from o...
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7699 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/10th022_027.pdf |
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| author | Ballester-Olmos, José F. Pina, José A. Moreno, Pedro Hermoso-De-Mendoza, Alfonso Cambra, Mariano Navarro, Luis |
| author2 | Timmer, L. W. |
| author_browse | Ballester-Olmos, José F. Cambra, Mariano Hermoso-De-Mendoza, Alfonso Moreno, Pedro Navarro, Luis Pina, José A. Timmer, L. W. |
| author_facet | Timmer, L. W. Ballester-Olmos, José F. Pina, José A. Moreno, Pedro Hermoso-De-Mendoza, Alfonso Cambra, Mariano Navarro, Luis |
| author_sort | Ballester-Olmos, José F. |
| collection | ReDivia |
| description | Sixty citrus tristeza virus (CTV) isolates representative of most Spanish citrus areas were collected and initially inoculated on Mexican lime and Duncan grapefruit. Ten of them were selected on the basis of origin, host and symptoms on those indicator plants. Selected isolates were separated from other virus and virus-like pathogens by transmission with Aphis gossypii from graft-inoculated sweet orange to Mexican lime seedlings. Purified isolates were inoculated into ten seedlings each of Duncan grapefruit and sour orange, 20 seedlings of sweet orange cvs. Pineapple and Comuna and 20 budlings of Washington navel orange grafted on sour orange. Inoculated Duncan grapefruit and sour orange seedlings were incubated in a temperature-controlled greenhouse maintained at 18º-26ºC. Sweet orange seedlings and navel orange budlings were split in two groups of ten plants each after inoculation. One of them was incubated under greenhouse conditions (18º-26ºC) and the other was kept in an insect-proof screenhouse. Vein clearing in young flushes and stem pitting intensity inoculation were evaluated one year after by three observers and their scores averaged. The following conclusions were drawn: 1) different pathotypes were found among the Spanish CTV isolates characterized; 2) none of the 60 CTV isolates initially inoculated on grapefruit gave a seedling yellows reaction; 3) none of the isolates studied induced stem pitting on Duncan grapefruit or sweet orange cvs. Pineapple or Comuna; 4) some differences in symptom expression were observed between plants incubated under greenhouse and screenhouse conditions. |
| format | Objeto de conferencia |
| id | ReDivia7699 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia76992025-04-25T14:52:39Z Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain Ballester-Olmos, José F. Pina, José A. Moreno, Pedro Hermoso-De-Mendoza, Alfonso Cambra, Mariano Navarro, Luis Timmer, L. W. Isolates H10 Pests of plants H20 Plant diseases Citrus tristeza virus Disease symptoms Aphis gossypii Sixty citrus tristeza virus (CTV) isolates representative of most Spanish citrus areas were collected and initially inoculated on Mexican lime and Duncan grapefruit. Ten of them were selected on the basis of origin, host and symptoms on those indicator plants. Selected isolates were separated from other virus and virus-like pathogens by transmission with Aphis gossypii from graft-inoculated sweet orange to Mexican lime seedlings. Purified isolates were inoculated into ten seedlings each of Duncan grapefruit and sour orange, 20 seedlings of sweet orange cvs. Pineapple and Comuna and 20 budlings of Washington navel orange grafted on sour orange. Inoculated Duncan grapefruit and sour orange seedlings were incubated in a temperature-controlled greenhouse maintained at 18º-26ºC. Sweet orange seedlings and navel orange budlings were split in two groups of ten plants each after inoculation. One of them was incubated under greenhouse conditions (18º-26ºC) and the other was kept in an insect-proof screenhouse. Vein clearing in young flushes and stem pitting intensity inoculation were evaluated one year after by three observers and their scores averaged. The following conclusions were drawn: 1) different pathotypes were found among the Spanish CTV isolates characterized; 2) none of the 60 CTV isolates initially inoculated on grapefruit gave a seedling yellows reaction; 3) none of the isolates studied induced stem pitting on Duncan grapefruit or sweet orange cvs. Pineapple or Comuna; 4) some differences in symptom expression were observed between plants incubated under greenhouse and screenhouse conditions. 2021-10-27T10:34:29Z 2021-10-27T10:34:29Z 1988 conferenceObject Ballester-Olmos, J. F., Pina, J. A., Moreno, P., Hermoso-de-Mendoza, A. Cambra, M. & Navarro, L. (1988). Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain. Proceedings of the Tenth Conference of the IOCV, 22-27. 2313-5123 0074-7203 (e-ISSN) http://hdl.handle.net/20.500.11939/7699 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/10th022_027.pdf en 1986-11-17 Tenth Conference of the International Organization of Citrus Virologists Valencia, España Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Isolates H10 Pests of plants H20 Plant diseases Citrus tristeza virus Disease symptoms Aphis gossypii Ballester-Olmos, José F. Pina, José A. Moreno, Pedro Hermoso-De-Mendoza, Alfonso Cambra, Mariano Navarro, Luis Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title | Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title_full | Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title_fullStr | Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title_full_unstemmed | Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title_short | Biological Characterization of Different Citrus Tristeza Virus Isolates in Spain |
| title_sort | biological characterization of different citrus tristeza virus isolates in spain |
| topic | Isolates H10 Pests of plants H20 Plant diseases Citrus tristeza virus Disease symptoms Aphis gossypii |
| url | http://hdl.handle.net/20.500.11939/7699 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/10th022_027.pdf |
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