Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca)
There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apric...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7681 https://www.mdpi.com/1422-0067/22/7/3411 |
| _version_ | 1855492338720178176 |
|---|---|
| author | Gómez-Martínez, Helena Gil-Muñoz, Francisco Bermejo, Almudena Zuriaga, Elena Badenes, María L. |
| author_browse | Badenes, María L. Bermejo, Almudena Gil-Muñoz, Francisco Gómez-Martínez, Helena Zuriaga, Elena |
| author_facet | Gómez-Martínez, Helena Gil-Muñoz, Francisco Bermejo, Almudena Zuriaga, Elena Badenes, María L. |
| author_sort | Gómez-Martínez, Helena |
| collection | ReDivia |
| description | There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apricot (Prunus armeniaca) is a Mediterranean fruit with high diversity and fruit quality properties, being an excellent source of polyphenol compounds. For a better understanding of the phenolic pathway in these fruits, we selected a set of accessions with genetic-based differences in phenolic compounds accumulation. HPLC analysis of the main phenolic compounds and transcriptional analysis of the genes involved in key steps of the polyphenol network were carried out. Phenylalanine ammonia-lyase (PAL), dihydroflavonol-4-reductase (DFR) and flavonol synthase (FLS) were the key enzymes selected. Orthologous of the genes involved in transcription of these enzymes were identified in apricot: ParPAL1, ParPAL2, ParDFR, ParFLS1 and ParFLS2. Transcriptional data of the genes involved in those critical points and their relationships with the polyphenol compounds were analyzed. Higher expression of ParDFR and ParPAL2 has been associated with red-blushed accessions. Differences in expression between paralogues could be related to the presence of a BOXCOREDCPAL cis-acting element related to the genes involved in anthocyanin synthesis ParFLS2, ParDFR and ParPAL2. |
| format | Artículo |
| id | ReDivia7681 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia76812025-04-25T14:48:28Z Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) Gómez-Martínez, Helena Gil-Muñoz, Francisco Bermejo, Almudena Zuriaga, Elena Badenes, María L. Phenolic pathway Flavonol synthase Dihydroflavonol-4-reductase (DFR) Phenyalanine ammonia-lyase (PAL) F30 Plant genetics and breeding F60 Plant physiology and biochemistry Q01 Food science and technology Q04 Food composition Fruits Polyphenols Apricots Genes Flavonoids There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apricot (Prunus armeniaca) is a Mediterranean fruit with high diversity and fruit quality properties, being an excellent source of polyphenol compounds. For a better understanding of the phenolic pathway in these fruits, we selected a set of accessions with genetic-based differences in phenolic compounds accumulation. HPLC analysis of the main phenolic compounds and transcriptional analysis of the genes involved in key steps of the polyphenol network were carried out. Phenylalanine ammonia-lyase (PAL), dihydroflavonol-4-reductase (DFR) and flavonol synthase (FLS) were the key enzymes selected. Orthologous of the genes involved in transcription of these enzymes were identified in apricot: ParPAL1, ParPAL2, ParDFR, ParFLS1 and ParFLS2. Transcriptional data of the genes involved in those critical points and their relationships with the polyphenol compounds were analyzed. Higher expression of ParDFR and ParPAL2 has been associated with red-blushed accessions. Differences in expression between paralogues could be related to the presence of a BOXCOREDCPAL cis-acting element related to the genes involved in anthocyanin synthesis ParFLS2, ParDFR and ParPAL2. 2021-10-21T14:22:40Z 2021-10-21T14:22:40Z 2021 article publishedVersion Gómez-Martínez, H., Gil-Muñoz, F., Bermejo, A., Zuriaga, E. & Badenes, M. L. (2021). Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca). International journal of molecular sciences, 22(7), 3411. 1422-0067 http://hdl.handle.net/20.500.11939/7681 10.3390/ijms22073411 https://www.mdpi.com/1422-0067/22/7/3411 en info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/RTA2017-00011-C03-01//Mejora de albaricoquero y melocotonero en un contexto de agricultura sostenible y cambio climático This research was funded by the Instituto Nacional de Investigation y Tecnología Agraria y Alimentaria (INIA)-FEDER (RTA2017-00011-C03-01) and co-funded by the European Union through the European Regional Development Fund (ERDF) of the Generalitat Valenciana 2014–2020. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Phenolic pathway Flavonol synthase Dihydroflavonol-4-reductase (DFR) Phenyalanine ammonia-lyase (PAL) F30 Plant genetics and breeding F60 Plant physiology and biochemistry Q01 Food science and technology Q04 Food composition Fruits Polyphenols Apricots Genes Flavonoids Gómez-Martínez, Helena Gil-Muñoz, Francisco Bermejo, Almudena Zuriaga, Elena Badenes, María L. Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title | Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title_full | Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title_fullStr | Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title_full_unstemmed | Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title_short | Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca) |
| title_sort | insights of phenolic pathway in fruits transcriptional and metabolic profiling in apricot prunus armeniaca |
| topic | Phenolic pathway Flavonol synthase Dihydroflavonol-4-reductase (DFR) Phenyalanine ammonia-lyase (PAL) F30 Plant genetics and breeding F60 Plant physiology and biochemistry Q01 Food science and technology Q04 Food composition Fruits Polyphenols Apricots Genes Flavonoids |
| url | http://hdl.handle.net/20.500.11939/7681 https://www.mdpi.com/1422-0067/22/7/3411 |
| work_keys_str_mv | AT gomezmartinezhelena insightsofphenolicpathwayinfruitstranscriptionalandmetabolicprofilinginapricotprunusarmeniaca AT gilmunozfrancisco insightsofphenolicpathwayinfruitstranscriptionalandmetabolicprofilinginapricotprunusarmeniaca AT bermejoalmudena insightsofphenolicpathwayinfruitstranscriptionalandmetabolicprofilinginapricotprunusarmeniaca AT zuriagaelena insightsofphenolicpathwayinfruitstranscriptionalandmetabolicprofilinginapricotprunusarmeniaca AT badenesmarial insightsofphenolicpathwayinfruitstranscriptionalandmetabolicprofilinginapricotprunusarmeniaca |