Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance
An improvement in reproduction management has been associated to the wide use of artificial insemination in rabbits. The use of frozen semen in this species is greatly limited due to its low fertility rates. Moreover, in politocous species, prolificacy is a very important trait and it is particularl...
| Autores principales: | , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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World Rabbit Science Association
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7666 http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/R-Cortell1.pdf |
| _version_ | 1855492335915237376 |
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| author | Cortell, Carmela Viudes-De-Castro, María P. |
| author_browse | Cortell, Carmela Viudes-De-Castro, María P. |
| author_facet | Cortell, Carmela Viudes-De-Castro, María P. |
| author_sort | Cortell, Carmela |
| collection | ReDivia |
| description | An improvement in reproduction management has been associated to the wide use of artificial insemination in rabbits. The use of frozen semen in this species is greatly limited due to its low fertility rates. Moreover, in politocous species, prolificacy is a very important trait and it is particularly affected when semen is frozen. Since gelatine addition to fresh semen extenders improves and
prolongs motility and viability sperm parameters, its use in semen freezing could enhance sperm efficiency after thawing. The aim of this study was to assess the effects of gelatine addition to freezing extender using a commercially available extender (MIII) and a tris-based extender (TGC). Four experimental extenders were used: TGC extender with gelatine, the same without gelatine and MIII extender with and without gelatine. In order to evaluate seminal parameters, motility was assessed using the Computer Assisted Sperm Analysis, while viability rate was calculated using flow cytometry. To evaluate in vivo frozen-thawed semen, 273 females were inseminated. No significant difference was found among the four freezing extenders when motility, viability fertility or prolificacy were considered. Motility rates ranged between 22.4% and 35.1%, while viability varied between 22.5 and 30.9%. Fertility rates oscillated from 80.8% to 86.2% and the total born ranged from 7.9 to 8.5. While in vitro results were lower than those obtained in other studies, in vivo results were quite favourable. In conclusion, gelatine addition did not improve sperm motility and viability after thawing nor fertility and prolificacy after insemination with frozen-thawed semen, using MIII or TCG extender. |
| format | Objeto de conferencia |
| id | ReDivia7666 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | World Rabbit Science Association |
| publisherStr | World Rabbit Science Association |
| record_format | dspace |
| spelling | ReDivia76662025-04-25T14:53:43Z Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance Cortell, Carmela Viudes-De-Castro, María P. Freezing extender L01 Animal husbandry L10 Animal genetics and breeding L53 Animal physiology - Reproduction Rabbits Gelatine Semen Reproductive performance An improvement in reproduction management has been associated to the wide use of artificial insemination in rabbits. The use of frozen semen in this species is greatly limited due to its low fertility rates. Moreover, in politocous species, prolificacy is a very important trait and it is particularly affected when semen is frozen. Since gelatine addition to fresh semen extenders improves and prolongs motility and viability sperm parameters, its use in semen freezing could enhance sperm efficiency after thawing. The aim of this study was to assess the effects of gelatine addition to freezing extender using a commercially available extender (MIII) and a tris-based extender (TGC). Four experimental extenders were used: TGC extender with gelatine, the same without gelatine and MIII extender with and without gelatine. In order to evaluate seminal parameters, motility was assessed using the Computer Assisted Sperm Analysis, while viability rate was calculated using flow cytometry. To evaluate in vivo frozen-thawed semen, 273 females were inseminated. No significant difference was found among the four freezing extenders when motility, viability fertility or prolificacy were considered. Motility rates ranged between 22.4% and 35.1%, while viability varied between 22.5 and 30.9%. Fertility rates oscillated from 80.8% to 86.2% and the total born ranged from 7.9 to 8.5. While in vitro results were lower than those obtained in other studies, in vivo results were quite favourable. In conclusion, gelatine addition did not improve sperm motility and viability after thawing nor fertility and prolificacy after insemination with frozen-thawed semen, using MIII or TCG extender. 2021-10-18T10:07:44Z 2021-10-18T10:07:44Z 2008 conferenceObject Cortell, C. & Viudes-de-Castro (2008). Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance. Proceedings of the 9th World Rabbit Congress, 327-331. http://hdl.handle.net/20.500.11939/7666 http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/R-Cortell1.pdf en 2008-06-10 9th World Rabbit Congress Verona, Italy Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess World Rabbit Science Association electronico |
| spellingShingle | Freezing extender L01 Animal husbandry L10 Animal genetics and breeding L53 Animal physiology - Reproduction Rabbits Gelatine Semen Reproductive performance Cortell, Carmela Viudes-De-Castro, María P. Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title | Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title_full | Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title_fullStr | Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title_full_unstemmed | Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title_short | Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| title_sort | effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance |
| topic | Freezing extender L01 Animal husbandry L10 Animal genetics and breeding L53 Animal physiology - Reproduction Rabbits Gelatine Semen Reproductive performance |
| url | http://hdl.handle.net/20.500.11939/7666 http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/R-Cortell1.pdf |
| work_keys_str_mv | AT cortellcarmela effectofgelatinadditiontofreezingextenderonrabbitsemenparametersandreproductiveperformance AT viudesdecastromariap effectofgelatinadditiontofreezingextenderonrabbitsemenparametersandreproductiveperformance |