Screening of virulence genes in Staphylococcus aureus isolates from rabbits
Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments and produce a wide range of diseases both in humans and animals. This versatility depends on its ability to modulate gene expression and the synthesis of virulence determinants. Therefore, this study aimed to...
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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World Rabbit Science Association
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7665 |
| _version_ | 1855492335726493696 |
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| author | Viana, David Selva, Laura García-Quirós, A. Penadés, M. Penadés, José R. Corpa, Juan M. |
| author_browse | Corpa, Juan M. García-Quirós, A. Penadés, José R. Penadés, M. Selva, Laura Viana, David |
| author_facet | Viana, David Selva, Laura García-Quirós, A. Penadés, M. Penadés, José R. Corpa, Juan M. |
| author_sort | Viana, David |
| collection | ReDivia |
| description | Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments and produce a wide range of diseases both in humans and animals. This versatility depends on its ability to modulate gene expression and the synthesis of virulence determinants. Therefore, this study aimed to investigate the distribution of bacterial virulence determinants in the most prevalent S. aureus strain types causing lesions in rabbits. Sixty-nine S. aureus strains were isolated from rabbit does with
different chronic purulent lesions from 30 Spanish industrial rabbitries. Genotyping characterization of the strains was performed based on the analysis of the polymorphic regions of the coa, spa and clfB genes, as well as Multylocus Sequence Typing (MLST) on one strain of each of the most frequent genotypes. The isolates were also analyzed for the presence of forty virulence genes by PCRs and Southern blot, in order to determine their relationship with the genotype and the type of infection respectively. The great majority of isolates belonging to the same genotype were related to the same virulence factors, even though certain virulence factors were variable inside a genotype. However, the type of infection could not be related to any combination of virulence factors. |
| format | Objeto de conferencia |
| id | ReDivia7665 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | World Rabbit Science Association |
| publisherStr | World Rabbit Science Association |
| record_format | dspace |
| spelling | ReDivia76652025-04-25T14:53:41Z Screening of virulence genes in Staphylococcus aureus isolates from rabbits Viana, David Selva, Laura García-Quirós, A. Penadés, M. Penadés, José R. Corpa, Juan M. Virulence factors Multilocus sequence typing L70 Veterinary science and hygiene L01 Animal husbandry L10 Animal genetics and breeding Staphylococcus aureus Rabbits Virulence Genotypes Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments and produce a wide range of diseases both in humans and animals. This versatility depends on its ability to modulate gene expression and the synthesis of virulence determinants. Therefore, this study aimed to investigate the distribution of bacterial virulence determinants in the most prevalent S. aureus strain types causing lesions in rabbits. Sixty-nine S. aureus strains were isolated from rabbit does with different chronic purulent lesions from 30 Spanish industrial rabbitries. Genotyping characterization of the strains was performed based on the analysis of the polymorphic regions of the coa, spa and clfB genes, as well as Multylocus Sequence Typing (MLST) on one strain of each of the most frequent genotypes. The isolates were also analyzed for the presence of forty virulence genes by PCRs and Southern blot, in order to determine their relationship with the genotype and the type of infection respectively. The great majority of isolates belonging to the same genotype were related to the same virulence factors, even though certain virulence factors were variable inside a genotype. However, the type of infection could not be related to any combination of virulence factors. 2021-10-18T10:07:31Z 2021-10-18T10:07:31Z 2012 conferenceObject Viana, D., Selva, L., García-Quirós, A., Penadés, M., Penadés, J. R. & Corpa, J. M. (2012). Screening of virulence genes in Staphylococcus aureusisolates from rabbits. Proceedings 10th World Rabbit Congress, 1175-1179. http://hdl.handle.net/20.500.11939/7665 en 2012-09-03 10 th World Rabbit Congress Sharm El-Sheikh, Egypt This study was supported by grants from the CEU Cardenal Herrera University (PRCEU-UCH01/08, 23/08, 33/09 and 01/11) Banco Santander/CEU-UCH: Copernicus Program (PRCEU-UCH/COOP 01/08), Generalitat Valenciana (GV05/202, ACOMP/2009/207, ACOMP/2010/062) and the Comisión Interministerial de Ciencia y Tecnología (AGL2008-00273/GAN and AGL2011-30170-CO2-02). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess World Rabbit Science Association electronico |
| spellingShingle | Virulence factors Multilocus sequence typing L70 Veterinary science and hygiene L01 Animal husbandry L10 Animal genetics and breeding Staphylococcus aureus Rabbits Virulence Genotypes Viana, David Selva, Laura García-Quirós, A. Penadés, M. Penadés, José R. Corpa, Juan M. Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title | Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title_full | Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title_fullStr | Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title_full_unstemmed | Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title_short | Screening of virulence genes in Staphylococcus aureus isolates from rabbits |
| title_sort | screening of virulence genes in staphylococcus aureus isolates from rabbits |
| topic | Virulence factors Multilocus sequence typing L70 Veterinary science and hygiene L01 Animal husbandry L10 Animal genetics and breeding Staphylococcus aureus Rabbits Virulence Genotypes |
| url | http://hdl.handle.net/20.500.11939/7665 |
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