Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes
Sterile insect technique (SIT) is used, among other biological control tools, as a sustainable measure for the management of Ceratitis capitata Wiedemann (Diptera: Tephritidae) in many agricultural regions where this pest can trigger severe economic impacts. The tendency of wild females to remate mu...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley Online Library
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7628 https://onlinelibrary.wiley.com/doi/full/10.1111/jen.12925 |
| _version_ | 1855492326643728384 |
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| author | Català-Oltra, Marta Llácer, Elena Dembilio, Óscar Pla, Ignacio Urbaneja, Alberto Pérez-Hedo, Mertixell |
| author_browse | Català-Oltra, Marta Dembilio, Óscar Llácer, Elena Pla, Ignacio Pérez-Hedo, Mertixell Urbaneja, Alberto |
| author_facet | Català-Oltra, Marta Llácer, Elena Dembilio, Óscar Pla, Ignacio Urbaneja, Alberto Pérez-Hedo, Mertixell |
| author_sort | Català-Oltra, Marta |
| collection | ReDivia |
| description | Sterile insect technique (SIT) is used, among other biological control tools, as a sustainable measure for the management of Ceratitis capitata Wiedemann (Diptera: Tephritidae) in many agricultural regions where this pest can trigger severe economic impacts. The tendency of wild females to remate multiple times has been deeply studied; it has been a common point of controversy when evaluating SIT programmes. Nevertheless, the remating potential of the released sterile males remains unknown. Here, under laboratory conditions, the remating capability of mass-reared sterile males was determined. Wild-type virgin females were offered to sterile males (Vienna-8 strain), which had the opportunity to mate up to four consecutive times. The remating assays were carried out at 24 hr, 48 hr, 4 days and 7 days after the first mating. At the end of each tested time period, males were divided according to their mating response, mated or unmated, and subsequently reused for the next round of mating assays. The frequency of successful remating in each tested time period was obtained. Insemination was confirmed by determining the sperm transfer in mated female spermathecae by quantitative real-time PCR. Our results demonstrate that 73% of the mass-reared sterile males were able to remate 24 hr after the first mating, 55% of which remated again the day after. Close to 25% of the V8 sterile males tended to copulate in all of the four mating opportunities. The qPCR analysis of the spermathecae contents verified an effective transfer of V8 sperm to wild females with every mating; 99% of copulations resulted in sperm transfer. These findings shed light on the remating potential of V8 sterile males, an aspect until now underestimated in many SIT programmes. |
| format | Artículo |
| id | ReDivia7628 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley Online Library |
| publisherStr | Wiley Online Library |
| record_format | dspace |
| spelling | ReDivia76282025-04-25T14:48:26Z Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes Català-Oltra, Marta Llácer, Elena Dembilio, Óscar Pla, Ignacio Urbaneja, Alberto Pérez-Hedo, Mertixell Irradiated males Mediterranean fruit fly Molecular techniques Multiple mating Spermatozoa quantification H10 Pests of plants Sterile insect technique (SIT) is used, among other biological control tools, as a sustainable measure for the management of Ceratitis capitata Wiedemann (Diptera: Tephritidae) in many agricultural regions where this pest can trigger severe economic impacts. The tendency of wild females to remate multiple times has been deeply studied; it has been a common point of controversy when evaluating SIT programmes. Nevertheless, the remating potential of the released sterile males remains unknown. Here, under laboratory conditions, the remating capability of mass-reared sterile males was determined. Wild-type virgin females were offered to sterile males (Vienna-8 strain), which had the opportunity to mate up to four consecutive times. The remating assays were carried out at 24 hr, 48 hr, 4 days and 7 days after the first mating. At the end of each tested time period, males were divided according to their mating response, mated or unmated, and subsequently reused for the next round of mating assays. The frequency of successful remating in each tested time period was obtained. Insemination was confirmed by determining the sperm transfer in mated female spermathecae by quantitative real-time PCR. Our results demonstrate that 73% of the mass-reared sterile males were able to remate 24 hr after the first mating, 55% of which remated again the day after. Close to 25% of the V8 sterile males tended to copulate in all of the four mating opportunities. The qPCR analysis of the spermathecae contents verified an effective transfer of V8 sperm to wild females with every mating; 99% of copulations resulted in sperm transfer. These findings shed light on the remating potential of V8 sterile males, an aspect until now underestimated in many SIT programmes. 2021-10-01T13:04:48Z 2021-10-01T13:04:48Z 2021 article acceptedVersion Catalá‐Oltra, M., Llácer, E., Dembilio, O., Pla, I., Urbaneja, A. & Pérez‐Hedo, M. (2021). Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes. Journal of Applied Entomology, 145(10), 958-965. 1439-0418 http://hdl.handle.net/20.500.11939/7628 10.1111/jen.12925 https://onlinelibrary.wiley.com/doi/full/10.1111/jen.12925 en Funding information: EU-FSE (Fondo Social Europeo); Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Spain, Grant/ Award Number: Subprogram DOC-INIA- CCAA; Conselleria de Agricultura, Desarrollo Rural, Emergencia Climática y Transición Ecológica of the Valencian Community, Grant/Award Number: IVIA-5916 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Wiley Online Library electronico |
| spellingShingle | Irradiated males Mediterranean fruit fly Molecular techniques Multiple mating Spermatozoa quantification H10 Pests of plants Català-Oltra, Marta Llácer, Elena Dembilio, Óscar Pla, Ignacio Urbaneja, Alberto Pérez-Hedo, Mertixell Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title | Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title_full | Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title_fullStr | Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title_full_unstemmed | Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title_short | Remating in Ceratitis capitata sterile males: Implications in sterile insect technique programmes |
| title_sort | remating in ceratitis capitata sterile males implications in sterile insect technique programmes |
| topic | Irradiated males Mediterranean fruit fly Molecular techniques Multiple mating Spermatozoa quantification H10 Pests of plants |
| url | http://hdl.handle.net/20.500.11939/7628 https://onlinelibrary.wiley.com/doi/full/10.1111/jen.12925 |
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