Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles
When zoophytophagous mirids (Hemiptera: Miridae) feed on tomato plants they activate both direct and indirect defense mechanisms, which include the release of herbivore induced plant volatiles (HIPVs). HIPVs are capable of activating defense mechanisms in healthy neighboring plants. In this work, we...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | article |
| Language: | Inglés |
| Published: |
Schweizerbart Science Publishers
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7564 https://www.schweizerbart.de/papers/entomologia/detail/prepub/98496/Eliciting_tomato_plant_defenses_by_exposure_to_herbivore_induced_plant_volatiles |
| _version_ | 1855032629569519616 |
|---|---|
| author | Pérez-Hedo, Mertixell Alonso-Valiente, Miquel Vacas, Sandra Gallego, Carolina Rambla, José L. Navarro-Llopis, Vicente Granell, Antonio Urbaneja, Alberto |
| author_browse | Alonso-Valiente, Miquel Gallego, Carolina Granell, Antonio Navarro-Llopis, Vicente Pérez-Hedo, Mertixell Rambla, José L. Urbaneja, Alberto Vacas, Sandra |
| author_facet | Pérez-Hedo, Mertixell Alonso-Valiente, Miquel Vacas, Sandra Gallego, Carolina Rambla, José L. Navarro-Llopis, Vicente Granell, Antonio Urbaneja, Alberto |
| author_sort | Pérez-Hedo, Mertixell |
| collection | ReDivia |
| description | When zoophytophagous mirids (Hemiptera: Miridae) feed on tomato plants they activate both direct and indirect defense mechanisms, which include the release of herbivore induced plant volatiles (HIPVs). HIPVs are capable of activating defense mechanisms in healthy neighboring plants. In this work, we investigated which of these mirid-induced HIPVs are responsible for inducing plant defenses. Healthy tomato plants were individually exposed to eight HIPVs [1-hexanol, (Z)-3-hexenol, (Z)-3-hexenyl acetate, (Z)-3-hexenyl propanoate, (Z)-3-hexenyl butanoate, hexyl butanoate, methyl jasmonate and methyl salicylate] for 24 hours. Then, the expression level of defensive genes was quantified. All HIPVs led to increased expression of defensive genes by the plant when compared to unexposed tomato plants. In a further step, (Z)-3-hexenyl propanoate and methyl salicylate were selected to study the response of four tomato key pests and one natural enemy to tomato plants previously exposed to both HIPVs relative to unexposed control plants. Plants previously exposed to both HIPVs were repellent to Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), attractive to the parasitoid Encarsia formosa Gahan (Hymenoptera: Aphelinidae) and indifferent to Tetranychus urticae Koch (Acari: Tetranychidae). The volatiles emitted by plants previously exposed to both selected volatiles were also determined. Increased levels of C5 and C6 fatty acid-derived volatile compounds and β-ionone were detected, confirming that both HIPVs significantly activated the lipoxygenase pathway. These results are the starting point to advance the use of volatile compounds as defense elicitors in tomato crops. |
| format | article |
| id | ReDivia7564 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Schweizerbart Science Publishers |
| publisherStr | Schweizerbart Science Publishers |
| record_format | dspace |
| spelling | ReDivia75642025-04-25T14:48:23Z Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles Pérez-Hedo, Mertixell Alonso-Valiente, Miquel Vacas, Sandra Gallego, Carolina Rambla, José L. Navarro-Llopis, Vicente Granell, Antonio Urbaneja, Alberto (Z)-3-hexenyl propanoate Methyl salicylate Tuta absoluta H10 Pests of plants Bemisia tabaci Frankliniella occidentalis Tetranychus urticae Encarsia formosa When zoophytophagous mirids (Hemiptera: Miridae) feed on tomato plants they activate both direct and indirect defense mechanisms, which include the release of herbivore induced plant volatiles (HIPVs). HIPVs are capable of activating defense mechanisms in healthy neighboring plants. In this work, we investigated which of these mirid-induced HIPVs are responsible for inducing plant defenses. Healthy tomato plants were individually exposed to eight HIPVs [1-hexanol, (Z)-3-hexenol, (Z)-3-hexenyl acetate, (Z)-3-hexenyl propanoate, (Z)-3-hexenyl butanoate, hexyl butanoate, methyl jasmonate and methyl salicylate] for 24 hours. Then, the expression level of defensive genes was quantified. All HIPVs led to increased expression of defensive genes by the plant when compared to unexposed tomato plants. In a further step, (Z)-3-hexenyl propanoate and methyl salicylate were selected to study the response of four tomato key pests and one natural enemy to tomato plants previously exposed to both HIPVs relative to unexposed control plants. Plants previously exposed to both HIPVs were repellent to Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), attractive to the parasitoid Encarsia formosa Gahan (Hymenoptera: Aphelinidae) and indifferent to Tetranychus urticae Koch (Acari: Tetranychidae). The volatiles emitted by plants previously exposed to both selected volatiles were also determined. Increased levels of C5 and C6 fatty acid-derived volatile compounds and β-ionone were detected, confirming that both HIPVs significantly activated the lipoxygenase pathway. These results are the starting point to advance the use of volatile compounds as defense elicitors in tomato crops. 2021-08-25T15:45:32Z 2021-08-25T15:45:32Z 2021 article acceptedVersion Pérez-Hedo, M., Alonso-Valiente, M., Vacas, S., Gallego, C., Rambla, J. L., Navarro-Llopis, V. et al. (2021). Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles. Entomologia Generalis. 41(3), 209-218. 0171-8177 http://hdl.handle.net/20.500.11939/7564 10.1127/entomologia/2021/1196 https://www.schweizerbart.de/papers/entomologia/detail/prepub/98496/Eliciting_tomato_plant_defenses_by_exposure_to_herbivore_induced_plant_volatiles en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Schweizerbart Science Publishers electronico |
| spellingShingle | (Z)-3-hexenyl propanoate Methyl salicylate Tuta absoluta H10 Pests of plants Bemisia tabaci Frankliniella occidentalis Tetranychus urticae Encarsia formosa Pérez-Hedo, Mertixell Alonso-Valiente, Miquel Vacas, Sandra Gallego, Carolina Rambla, José L. Navarro-Llopis, Vicente Granell, Antonio Urbaneja, Alberto Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title | Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title_full | Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title_fullStr | Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title_full_unstemmed | Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title_short | Eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| title_sort | eliciting tomato plant defenses by exposure to herbivore induced plant volatiles |
| topic | (Z)-3-hexenyl propanoate Methyl salicylate Tuta absoluta H10 Pests of plants Bemisia tabaci Frankliniella occidentalis Tetranychus urticae Encarsia formosa |
| url | http://hdl.handle.net/20.500.11939/7564 https://www.schweizerbart.de/papers/entomologia/detail/prepub/98496/Eliciting_tomato_plant_defenses_by_exposure_to_herbivore_induced_plant_volatiles |
| work_keys_str_mv | AT perezhedomertixell elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT alonsovalientemiquel elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT vacassandra elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT gallegocarolina elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT ramblajosel elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT navarrollopisvicente elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT granellantonio elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles AT urbanejaalberto elicitingtomatoplantdefensesbyexposuretoherbivoreinducedplantvolatiles |