Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant
The ribosomal-depleted total RNA from white-berry grapevine (Vitis vinifera, SK933) plant showing severe chlorosis and downrolling of leaves was used for the high-throughput sequencing (HTS) analysis in order to unravel the potential contribution of the viral pathogens to the symptomatology observed...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Faculty of Horticulture and Landscape Engineering/Slovak University of Agriculture in Nitra.
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7412 https://www.sciendo.com/article/10.2478/ahr-2020-0009 |
| _version_ | 1855032601713049600 |
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| author | Glasa, Miroslav Predajna, Lukas Sihelská, Nina Soltys, Katarína Ruiz-García, Ana B. |
| author_browse | Glasa, Miroslav Predajna, Lukas Ruiz-García, Ana B. Sihelská, Nina Soltys, Katarína |
| author_facet | Glasa, Miroslav Predajna, Lukas Sihelská, Nina Soltys, Katarína Ruiz-García, Ana B. |
| author_sort | Glasa, Miroslav |
| collection | ReDivia |
| description | The ribosomal-depleted total RNA from white-berry grapevine (Vitis vinifera, SK933) plant showing severe chlorosis and downrolling of leaves was used for the high-throughput sequencing (HTS) analysis in order to unravel the potential contribution of the viral pathogens to the symptomatology observed. The combination of de novo assembly and mapping of ca. 1.1 millions of HTS reads enabled to identify and characterise a complex viral/viroid infection involving Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine rupestris vein feathering virus (GRVFV), Grapevine Syrah virus-1 (GSyV-1) and Hop stunt viroid (HSVd). The determined nearly complete genomes of GLRaV-2 SK933 showed its high genetic divergence from previously characterised isolates. In case of GRSPaV, two variants representing different evolutionary lineages have been identified in the plant. The results further pinpoint the complexity of grapevine viral diseases and show that mixed virus infection of grapevine is rather a rule than an exception. |
| format | article |
| id | ReDivia7412 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Faculty of Horticulture and Landscape Engineering/Slovak University of Agriculture in Nitra. |
| publisherStr | Faculty of Horticulture and Landscape Engineering/Slovak University of Agriculture in Nitra. |
| record_format | dspace |
| spelling | ReDivia74122025-04-25T14:48:19Z Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant Glasa, Miroslav Predajna, Lukas Sihelská, Nina Soltys, Katarína Ruiz-García, Ana B. Diversity Next generation sequencing H20 Plant diseases Vitis vinifera Viruses The ribosomal-depleted total RNA from white-berry grapevine (Vitis vinifera, SK933) plant showing severe chlorosis and downrolling of leaves was used for the high-throughput sequencing (HTS) analysis in order to unravel the potential contribution of the viral pathogens to the symptomatology observed. The combination of de novo assembly and mapping of ca. 1.1 millions of HTS reads enabled to identify and characterise a complex viral/viroid infection involving Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine rupestris vein feathering virus (GRVFV), Grapevine Syrah virus-1 (GSyV-1) and Hop stunt viroid (HSVd). The determined nearly complete genomes of GLRaV-2 SK933 showed its high genetic divergence from previously characterised isolates. In case of GRSPaV, two variants representing different evolutionary lineages have been identified in the plant. The results further pinpoint the complexity of grapevine viral diseases and show that mixed virus infection of grapevine is rather a rule than an exception. 2021-06-08T13:05:59Z 2021-06-08T13:05:59Z 2020 article publishedVersion Glasa, M., Predajňa, L., Sihelská, N., Šoltys, K., & Ruiz-García, A. B. (2020). Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant. Acta Horticulturae et Regiotecturae, 23(1), 35-39. 1335-2563 (printed edition) 1338-5259 (online) http://hdl.handle.net/20.500.11939/7412 10.2478/ahr-2020-0009 https://www.sciendo.com/article/10.2478/ahr-2020-0009 en This work was supported by the grant VEGA 2/0030/20 from the Scientific Grant Agency of the Ministry of Education and Slovak Academy of Sciences. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Faculty of Horticulture and Landscape Engineering/Slovak University of Agriculture in Nitra. electronico |
| spellingShingle | Diversity Next generation sequencing H20 Plant diseases Vitis vinifera Viruses Glasa, Miroslav Predajna, Lukas Sihelská, Nina Soltys, Katarína Ruiz-García, Ana B. Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title | Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title_full | Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title_fullStr | Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title_full_unstemmed | Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title_short | Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant |
| title_sort | analysis of virome by high throughput sequencing revealed multiple infection and intra virus diversity in a single grapevine plant |
| topic | Diversity Next generation sequencing H20 Plant diseases Vitis vinifera Viruses |
| url | http://hdl.handle.net/20.500.11939/7412 https://www.sciendo.com/article/10.2478/ahr-2020-0009 |
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