Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics
Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This s...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7350 https://www.mdpi.com/2076-2615/11/4/1178 |
| _version_ | 1855032590795276288 |
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| author | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José Salvador Marín, Clara |
| author_browse | Marco-Jiménez, Francisco Marín, Clara Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José Salvador Marín, Clara |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 °C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders |
| format | article |
| id | ReDivia7350 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia73502025-04-25T14:48:17Z Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José Salvador Marín, Clara Antibacterial activity Semen quality L73 Animal diseases L70 Veterinary science and hygiene Artificial insemination Antibiotics Reproductive performance Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 °C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders 2021-05-13T06:32:40Z 2021-05-13T06:32:40Z 2021 article publishedVersion Viudes-de-Castro, M.P., Marco-Jimenez, F., Vicente, J.S. & Marin, C. (2021). Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics.,11(4), 1178. 2076-2615 http://hdl.handle.net/20.500.11939/7350 10.3390/ani11041178 https://www.mdpi.com/2076-2615/11/4/1178 en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/AGL2017-85162-C2-1-R//MEJORA GENETICA DEL CONEJO DE CARNE: ESTRATEGIAS PARA INCREMENTAR LA EFICACIA DE LA MEJORA, REPRODUCCION Y SALUD DE LINEAS PATERNALES Funding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2017-85162-C2-1-R) Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Antibacterial activity Semen quality L73 Animal diseases L70 Veterinary science and hygiene Artificial insemination Antibiotics Reproductive performance Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José Salvador Marín, Clara Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title | Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title_full | Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title_fullStr | Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title_full_unstemmed | Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title_short | Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics |
| title_sort | antibacterial activity of some molecules added to rabbit semen extender as alternative to antibiotics |
| topic | Antibacterial activity Semen quality L73 Animal diseases L70 Veterinary science and hygiene Artificial insemination Antibiotics Reproductive performance |
| url | http://hdl.handle.net/20.500.11939/7350 https://www.mdpi.com/2076-2615/11/4/1178 |
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