Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance
Effects were analysed of dextran supplementation to Me2SO and acetamide rabbit semen freezing extenders on quality characteristics of rabbit spermatozoa and reproductive performance. The final concentration of cryoprotectants in pooled semen samples was 12.4 % Me2SO for the A extenders, 10.7 % Me2SO...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/7335 https://www.sciencedirect.com/science/article/abs/pii/S0378432021000294 |
| _version_ | 1855492264098267136 |
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| author | Viudes-De-Castro, María P. Talavan, Amparo Vicente, José Salvador |
| author_browse | Talavan, Amparo Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Talavan, Amparo Vicente, José Salvador |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | Effects were analysed of dextran supplementation to Me2SO and acetamide rabbit semen freezing extenders on quality characteristics of rabbit spermatozoa and reproductive performance. The final concentration of cryoprotectants in pooled semen samples was 12.4 % Me2SO for the A extenders, 10.7 % Me2SO and 2.9 % acetamide for the D extenders and 8.9 % Me2SO and 2.9 % acetamide in F extenders, with a supplementation of 1.7 % sucrose in all cases. There was not inclusion of dextran in the A0, D0, F0; while 5 % dextran was included in A5, D5, F5 and 10 % dextran in A10, D10 and F10 extenders. Sperm motility and viability rates were similar with use of the different extenders. Acrosome integrity after the freeze-thawing processes, however, was markedly greater when there was dextran supplementation of D and F extenders. Prolificacy was affected by extender composition. When there was artificial insemination (AI) using semen cryopreserved in the A extenders, number of kits born was similar to when there was AI with fresh semen when there was inclusion of 5% dextran for cryopreservation, while there was no effect on prolificacy when there was cryopreservation of semen using the D and F extenders. In conclusion, dextran supplementation of extenders containing Me2SO and acetamide resulted in greater acrosome integrity. Furthermore, when there was AI using sperm preserved in cryo-diluents containing an intermediate concentration of Me2SO, combined with inclusion of 5 % dextran, there was a marked beneficial effect on rabbit doe reproductive performance. |
| format | Artículo |
| id | ReDivia7335 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia73352025-04-25T14:48:16Z Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance Viudes-De-Castro, María P. Talavan, Amparo Vicente, José Salvador L53 Animal physiology - Reproduction L10 Animal genetics and breeding Sperm Rabbits Cryopreservation Dextrans Effects were analysed of dextran supplementation to Me2SO and acetamide rabbit semen freezing extenders on quality characteristics of rabbit spermatozoa and reproductive performance. The final concentration of cryoprotectants in pooled semen samples was 12.4 % Me2SO for the A extenders, 10.7 % Me2SO and 2.9 % acetamide for the D extenders and 8.9 % Me2SO and 2.9 % acetamide in F extenders, with a supplementation of 1.7 % sucrose in all cases. There was not inclusion of dextran in the A0, D0, F0; while 5 % dextran was included in A5, D5, F5 and 10 % dextran in A10, D10 and F10 extenders. Sperm motility and viability rates were similar with use of the different extenders. Acrosome integrity after the freeze-thawing processes, however, was markedly greater when there was dextran supplementation of D and F extenders. Prolificacy was affected by extender composition. When there was artificial insemination (AI) using semen cryopreserved in the A extenders, number of kits born was similar to when there was AI with fresh semen when there was inclusion of 5% dextran for cryopreservation, while there was no effect on prolificacy when there was cryopreservation of semen using the D and F extenders. In conclusion, dextran supplementation of extenders containing Me2SO and acetamide resulted in greater acrosome integrity. Furthermore, when there was AI using sperm preserved in cryo-diluents containing an intermediate concentration of Me2SO, combined with inclusion of 5 % dextran, there was a marked beneficial effect on rabbit doe reproductive performance. 2021-05-11T12:49:33Z 2021-05-11T12:49:33Z 2021 article acceptedVersion Viudes-de-Castro, M. P., Talaván, A. G., & Vicente, J. S. (2021). Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen–thawed rabbit sperm quality variables and reproductive performance. Animal Reproduction Science, 226, 106714. 0378-4320 http://hdl.handle.net/20.500.11939/7335 10.1016/j.anireprosci.2021.106714 https://www.sciencedirect.com/science/article/abs/pii/S0378432021000294 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Elsevier electronico |
| spellingShingle | L53 Animal physiology - Reproduction L10 Animal genetics and breeding Sperm Rabbits Cryopreservation Dextrans Viudes-De-Castro, María P. Talavan, Amparo Vicente, José Salvador Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title | Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title_full | Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title_fullStr | Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title_full_unstemmed | Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title_short | Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen-thawed rabbit sperm quality variables and reproductive performance |
| title_sort | evaluation of dextran for rabbit sperm cryopreservation effect on frozen thawed rabbit sperm quality variables and reproductive performance |
| topic | L53 Animal physiology - Reproduction L10 Animal genetics and breeding Sperm Rabbits Cryopreservation Dextrans |
| url | http://hdl.handle.net/20.500.11939/7335 https://www.sciencedirect.com/science/article/abs/pii/S0378432021000294 |
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