The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus
Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cuc...
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/7334 https://www.frontiersin.org/articles/10.3389/fmicb.2021.645530/full |
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| author | Domingo-Calap, María L. Chase, Ornela Estapé, Mariona Moreno, Ana Beatriz López-Moya, Juan J. |
| author_browse | Chase, Ornela Domingo-Calap, María L. Estapé, Mariona López-Moya, Juan J. Moreno, Ana Beatriz |
| author_facet | Domingo-Calap, María L. Chase, Ornela Estapé, Mariona Moreno, Ana Beatriz López-Moya, Juan J. |
| author_sort | Domingo-Calap, María L. |
| collection | ReDivia |
| description | Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in Nicotiana benthamiana leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Analysis of protein expression during the assays showed that the amount of P25 was not reduced when co-expressed with P1. The detrimental action of P1 on the activity of P25 was dose-dependent, and the subcellular localization of fluorescently labeled variants of P1 and P25 when transiently co-expressed showed coincidences both in nucleus and cytoplasm. Also, immunoprecipitation experiments showed interaction of tagged versions of the two proteins. This novel interaction, not previously described in other combinations of potyviruses and criniviruses, might play a role in modulating the complexities of the response to multiple viral infections in susceptible plants. |
| format | Artículo |
| id | ReDivia7334 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia73342025-04-25T14:48:16Z The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus Domingo-Calap, María L. Chase, Ornela Estapé, Mariona Moreno, Ana Beatriz López-Moya, Juan J. Watermelon mosaic virus Cucurbit yellow stunting disorder virus H20 Plant diseases F30 Plant genetics and breeding F60 Plant physiology and biochemistry Watermelons Potyviruses Plant viruses Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in Nicotiana benthamiana leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Analysis of protein expression during the assays showed that the amount of P25 was not reduced when co-expressed with P1. The detrimental action of P1 on the activity of P25 was dose-dependent, and the subcellular localization of fluorescently labeled variants of P1 and P25 when transiently co-expressed showed coincidences both in nucleus and cytoplasm. Also, immunoprecipitation experiments showed interaction of tagged versions of the two proteins. This novel interaction, not previously described in other combinations of potyviruses and criniviruses, might play a role in modulating the complexities of the response to multiple viral infections in susceptible plants. 2021-05-07T13:25:14Z 2021-05-07T13:25:14Z 2021 article publishedVersion Domingo-Calap, M. L., Chase, O., Estapé, M., Moreno, A. B., & López-Moya, J. J. (2021). The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus. Frontiers in Microbiology, 12, 622. 1664-302X (e-ISSN) http://hdl.handle.net/20.500.11939/7334 10.3389/fmicb.2021.645530 https://www.frontiersin.org/articles/10.3389/fmicb.2021.645530/full en info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-75529-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105692RB-100 Work in the author’s laboratory was financed by grants AGL2016-75529-R and PID2019-105692RB-100 from Spanish Ministry of Science and Innovation MD-C was supported by FPI contract BES-2014-068970 from Ministry of Science and Innovation and FEDER OC was supported by 2018 FI_B 00329 from AGAUR, Generalitat de Catalunya, and Fons Social Europeu AM was supported by European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 657527 We acknowledge financial support from the Spanish Ministry of Science and Innovation-State Research Agency (AEI), through the “Severo Ochoa Programme for Centres of Excellence in R&D” SEV-2015-0533 and CEX2019-000902-S, and from Generalitat de Catalunya CERCA Institution and support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Frontiers Media electronico |
| spellingShingle | Watermelon mosaic virus Cucurbit yellow stunting disorder virus H20 Plant diseases F30 Plant genetics and breeding F60 Plant physiology and biochemistry Watermelons Potyviruses Plant viruses Domingo-Calap, María L. Chase, Ornela Estapé, Mariona Moreno, Ana Beatriz López-Moya, Juan J. The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title | The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title_full | The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title_fullStr | The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title_full_unstemmed | The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title_short | The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus |
| title_sort | p1 protein of watermelon mosaic virus compromises the activity as rna silencing suppressor of the p25 protein of cucurbit yellow stunting disorder virus |
| topic | Watermelon mosaic virus Cucurbit yellow stunting disorder virus H20 Plant diseases F30 Plant genetics and breeding F60 Plant physiology and biochemistry Watermelons Potyviruses Plant viruses |
| url | http://hdl.handle.net/20.500.11939/7334 https://www.frontiersin.org/articles/10.3389/fmicb.2021.645530/full |
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