Genes impinging on tolerance to seasonal abiotic stresses in peach
Dormancy plays a crucial role in plant meristem survival during the cold period in temperate climates. Meristem growth cessation and specific mechanisms for stress tolerance operate during this period, alleviating the deleterious effect of low temperature and water deprivation. We have performed gen...
| Autores principales: | , , |
|---|---|
| Otros Autores: | |
| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
ISHS
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7058 https://www.actahort.org/books/1280/1280_25.htm |
| _version_ | 1855492208245866496 |
|---|---|
| author | Lloret, Alba Badenes, María L. Ríos, Gabino |
| author2 | Sanal, D. |
| author_browse | Badenes, María L. Lloret, Alba Ríos, Gabino Sanal, D. |
| author_facet | Sanal, D. Lloret, Alba Badenes, María L. Ríos, Gabino |
| author_sort | Lloret, Alba |
| collection | ReDivia |
| description | Dormancy plays a crucial role in plant meristem survival during the cold period in temperate climates. Meristem growth cessation and specific mechanisms for stress tolerance operate during this period, alleviating the deleterious effect of low temperature and water deprivation. We have performed gene expression studies in buds undergoing dormancy release in order to identify putative factors and pathways involved in stress tolerance in dormant buds. We have identified PpSAP1 and PpeS6PDH genes coding respectively for a stress associated protein-like and a sorbitol-6-phosphate dehydrogenase enzyme, both of which are downregulated in dormancy released flower buds of peach. Overexpression of PpSAP1 in transgenic plums reduces water loss under desiccation conditions and impairs cell growth. Gene expression studies indicate PpSAP1 could modulate water loss and cell growth by an ubiquitin-dependent mechanism affecting transcriptional regulation of specific targets. On the other hand, PpeS6PDH is a key enzyme involved in sorbitol synthesis, as stated by biochemical assays of purified recombinant protein. PpeS6PDH shows higher expression in dormant buds, in concordance with sorbitol accumulation data. Sorbitol has been described as a compatible solute and cryoprotectant in different species, which suggests its participation in freezing and water stress tolerance mechanisms in dormant buds. |
| format | Objeto de conferencia |
| id | ReDivia7058 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | ISHS |
| publisherStr | ISHS |
| record_format | dspace |
| spelling | ReDivia70582025-04-25T14:53:56Z Genes impinging on tolerance to seasonal abiotic stresses in peach Lloret, Alba Badenes, María L. Ríos, Gabino Sanal, D. Stress associated protein F30 Plant genetics and breeding Dormancy Sorbitol Drought Gene expression Dormancy plays a crucial role in plant meristem survival during the cold period in temperate climates. Meristem growth cessation and specific mechanisms for stress tolerance operate during this period, alleviating the deleterious effect of low temperature and water deprivation. We have performed gene expression studies in buds undergoing dormancy release in order to identify putative factors and pathways involved in stress tolerance in dormant buds. We have identified PpSAP1 and PpeS6PDH genes coding respectively for a stress associated protein-like and a sorbitol-6-phosphate dehydrogenase enzyme, both of which are downregulated in dormancy released flower buds of peach. Overexpression of PpSAP1 in transgenic plums reduces water loss under desiccation conditions and impairs cell growth. Gene expression studies indicate PpSAP1 could modulate water loss and cell growth by an ubiquitin-dependent mechanism affecting transcriptional regulation of specific targets. On the other hand, PpeS6PDH is a key enzyme involved in sorbitol synthesis, as stated by biochemical assays of purified recombinant protein. PpeS6PDH shows higher expression in dormant buds, in concordance with sorbitol accumulation data. Sorbitol has been described as a compatible solute and cryoprotectant in different species, which suggests its participation in freezing and water stress tolerance mechanisms in dormant buds. 2021-02-02T14:40:57Z 2021-02-02T14:40:57Z 2020 conferenceObject Lloret, A., Badenes, M. L. & Ríos, G. (2020). Genes impinging on tolerance to seasonal abiotic stresses in peach. Acta Horticulturae, 1280, 183-188. 978-94-62612-78-5 0567-7572 (print) 2406-6168 (electronic) http://hdl.handle.net/20.500.11939/7058 10.17660/ActaHortic.2020.1280.25 https://www.actahort.org/books/1280/1280_25.htm en 2018-08-12 XXX International Horticultural Congress IHC2018: International Symposium on Nuts and Mediterranean Climate Fruits, Carob and X International Symposium on Temperate Fruits in the Tropics and Subtropics Istanbul (Turkey) Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess ISHS electronico |
| spellingShingle | Stress associated protein F30 Plant genetics and breeding Dormancy Sorbitol Drought Gene expression Lloret, Alba Badenes, María L. Ríos, Gabino Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title | Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title_full | Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title_fullStr | Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title_full_unstemmed | Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title_short | Genes impinging on tolerance to seasonal abiotic stresses in peach |
| title_sort | genes impinging on tolerance to seasonal abiotic stresses in peach |
| topic | Stress associated protein F30 Plant genetics and breeding Dormancy Sorbitol Drought Gene expression |
| url | http://hdl.handle.net/20.500.11939/7058 https://www.actahort.org/books/1280/1280_25.htm |
| work_keys_str_mv | AT lloretalba genesimpingingontolerancetoseasonalabioticstressesinpeach AT badenesmarial genesimpingingontolerancetoseasonalabioticstressesinpeach AT riosgabino genesimpingingontolerancetoseasonalabioticstressesinpeach |