Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest
Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout theMediterranean Basin in tomato crops but regarded as a pest in northern European countries. Fromthe familyMiridae, it is an economically important insect yet very little is known in terms of geneti...
| Autores principales: | , , , , , , , , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7007 https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12688 |
| _version_ | 1855032529673781248 |
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| author | Ferguson, Kim B. Visser, Sander Daliková, M. Provazniková, I. Urbaneja, Alberto Pérez-Hedo, Mertixell Marec, F. Werren, John H. Zwaan, Bas J. Pannebakker, Bart A. Verhulst, Eveline C. |
| author_browse | Daliková, M. Ferguson, Kim B. Marec, F. Pannebakker, Bart A. Provazniková, I. Pérez-Hedo, Mertixell Urbaneja, Alberto Verhulst, Eveline C. Visser, Sander Werren, John H. Zwaan, Bas J. |
| author_facet | Ferguson, Kim B. Visser, Sander Daliková, M. Provazniková, I. Urbaneja, Alberto Pérez-Hedo, Mertixell Marec, F. Werren, John H. Zwaan, Bas J. Pannebakker, Bart A. Verhulst, Eveline C. |
| author_sort | Ferguson, Kim B. |
| collection | ReDivia |
| description | Nesidiocoris tenuis (Reuter) is an efficient predatory biological
control agent used throughout theMediterranean
Basin in tomato crops but regarded as a pest in northern
European countries. Fromthe familyMiridae, it is an economically
important insect yet very little is known in
terms of genetic information and no genomic or transcriptomic
studies have been published. Here, we use
a linked-read sequencing strategy on a single female
N. tenuis. From this, we assembled the 355 Mbp genome
and delivered an ab initio, homology-based and
evidence-based annotation. Along the way, the bacterial
“contamination” was removed from the assembly. In
addition, bacterial lateral gene transfer (LGT) candidates
were detected in the N. tenuis genome. The complete
gene set is composed of 24 688 genes; the associated
proteins were compared to other hemipterans (Cimex
lectularis, Halyomorpha halys and Acyrthosiphon
pisum).We visualized the genome using various cytogenetic
techniques, such as karyotyping, CGH and GISH,
indicating a karyotype of 2n = 32. Additional analyses
include the localization of 18S rDNA and unique satellite
probes as well as pooled sequencing to assess nucleotide
diversity and neutrality of the commercial population.
This is one of the first mirid genomes to be
released and the first of a mirid biological control agent |
| format | publishedVersion |
| id | ReDivia7007 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | ReDivia70072025-04-25T14:48:04Z Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest Ferguson, Kim B. Visser, Sander Daliková, M. Provazniková, I. Urbaneja, Alberto Pérez-Hedo, Mertixell Marec, F. Werren, John H. Zwaan, Bas J. Pannebakker, Bart A. Verhulst, Eveline C. Hemiptera linked-read biocontrol H10 Pests of plants Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout theMediterranean Basin in tomato crops but regarded as a pest in northern European countries. Fromthe familyMiridae, it is an economically important insect yet very little is known in terms of genetic information and no genomic or transcriptomic studies have been published. Here, we use a linked-read sequencing strategy on a single female N. tenuis. From this, we assembled the 355 Mbp genome and delivered an ab initio, homology-based and evidence-based annotation. Along the way, the bacterial “contamination” was removed from the assembly. In addition, bacterial lateral gene transfer (LGT) candidates were detected in the N. tenuis genome. The complete gene set is composed of 24 688 genes; the associated proteins were compared to other hemipterans (Cimex lectularis, Halyomorpha halys and Acyrthosiphon pisum).We visualized the genome using various cytogenetic techniques, such as karyotyping, CGH and GISH, indicating a karyotype of 2n = 32. Additional analyses include the localization of 18S rDNA and unique satellite probes as well as pooled sequencing to assess nucleotide diversity and neutrality of the commercial population. This is one of the first mirid genomes to be released and the first of a mirid biological control agent 2021-01-20T08:17:34Z 2021-01-20T08:17:34Z 2021 publishedVersion Ferguson, K.B., Visser, S., Dalíková, M., Provazníková, I., Urbaneja, A., Pérez‐Hedo, M., Marec, F., Werren, J.H., Zwaan, B.J., Pannebakker, B.A. and Verhulst, E.C. (2021), Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest. Insect Mol Biol. http://hdl.handle.net/20.500.11939/7007 doi: 10.1111/imb.12688 https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12688 en openAccess Wiley electronico |
| spellingShingle | Hemiptera linked-read biocontrol H10 Pests of plants Ferguson, Kim B. Visser, Sander Daliková, M. Provazniková, I. Urbaneja, Alberto Pérez-Hedo, Mertixell Marec, F. Werren, John H. Zwaan, Bas J. Pannebakker, Bart A. Verhulst, Eveline C. Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title | Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title_full | Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title_fullStr | Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title_full_unstemmed | Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title_short | Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest |
| title_sort | jekyll or hyde the genome and more of nesidiocoris tenuis a zoophytophagous predatory bug that is both a biological control agent and a pest |
| topic | Hemiptera linked-read biocontrol H10 Pests of plants |
| url | http://hdl.handle.net/20.500.11939/7007 https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12688 |
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