Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.)
Agriculture needs solutions for adapting crops to increasing salinity globally. Research on physiological and molecular responses activated by salinity is needed to elucidate mechanisms of salinity tolerance. Transcriptome profiling (RNA-Seq) is a powerful tool to study the transcriptomic profile of...
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6966 https://www.mdpi.com/2073-4395/10/11/1703 |
| _version_ | 1855492192029638656 |
|---|---|
| author | Gil-Muñoz, Francisco Delhomme, Nicolas Quinones, Ana Naval, María M. Badenes, María L. García-Gil, M. Rosario |
| author_browse | Badenes, María L. Delhomme, Nicolas García-Gil, M. Rosario Gil-Muñoz, Francisco Naval, María M. Quinones, Ana |
| author_facet | Gil-Muñoz, Francisco Delhomme, Nicolas Quinones, Ana Naval, María M. Badenes, María L. García-Gil, M. Rosario |
| author_sort | Gil-Muñoz, Francisco |
| collection | ReDivia |
| description | Agriculture needs solutions for adapting crops to increasing salinity globally. Research on physiological and molecular responses activated by salinity is needed to elucidate mechanisms of salinity tolerance. Transcriptome profiling (RNA-Seq) is a powerful tool to study the transcriptomic profile of genotypes under stress conditions. Persimmon species have different levels of tolerance to salinity, this variability may provide knowledge on persimmon species and development of salt--tolerant rootstocks. In this study, we conducted a physiological and transcriptomic profiling of roots and leaves in tolerant and sensitive plants of persimmon rootstock grown under saline and control conditions. Characterization of physiological responses along with gene expression changes in roots and leaves allowed the identification of several salt tolerance mechanisms related to ion transport and thermospermine synthesis. Differences were observed in putative H+/ATPases that allow transmembrane ionic transport and chloride channel protein-like genes. Furthermore, an overexpression of thermospermine synthase found in the roots of tolerant plants may indicate that alterations in root architecture could act as an additional mechanism of response to salt stress. These results indicate that Diospyros lotus L. exhibits genetically-controlled variability for salt tolerance traits which opens potential opportunities for breeding salt-tolerant persimmon rootstocks in a Mediterranean environment challenged by drought and salinity. |
| format | Artículo |
| id | ReDivia6966 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia69662025-04-25T14:48:00Z Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) Gil-Muñoz, Francisco Delhomme, Nicolas Quinones, Ana Naval, María M. Badenes, María L. García-Gil, M. Rosario Ion transport Persimmon Transmembrane electrochemical gradient Chloride channels Thermospermine P33 Soil chemistry and physics F60 Plant physiology and biochemistry Diospyros lotus Salt stress Agriculture needs solutions for adapting crops to increasing salinity globally. Research on physiological and molecular responses activated by salinity is needed to elucidate mechanisms of salinity tolerance. Transcriptome profiling (RNA-Seq) is a powerful tool to study the transcriptomic profile of genotypes under stress conditions. Persimmon species have different levels of tolerance to salinity, this variability may provide knowledge on persimmon species and development of salt--tolerant rootstocks. In this study, we conducted a physiological and transcriptomic profiling of roots and leaves in tolerant and sensitive plants of persimmon rootstock grown under saline and control conditions. Characterization of physiological responses along with gene expression changes in roots and leaves allowed the identification of several salt tolerance mechanisms related to ion transport and thermospermine synthesis. Differences were observed in putative H+/ATPases that allow transmembrane ionic transport and chloride channel protein-like genes. Furthermore, an overexpression of thermospermine synthase found in the roots of tolerant plants may indicate that alterations in root architecture could act as an additional mechanism of response to salt stress. These results indicate that Diospyros lotus L. exhibits genetically-controlled variability for salt tolerance traits which opens potential opportunities for breeding salt-tolerant persimmon rootstocks in a Mediterranean environment challenged by drought and salinity. 2021-01-13T14:38:59Z 2021-01-13T14:38:59Z 2020 article publishedVersion Gil-Muñoz, F., Delhomme, N., Quiñones, A., Naval, M. M., Badenes, M. L. & García-Gil, M. R. (2020). Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus). Agronomy, 10(11), 1703. 2073-4395 http://hdl.handle.net/20.500.11939/6966 10.3390/agronomy10111703 https://www.mdpi.com/2073-4395/10/11/1703 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Ion transport Persimmon Transmembrane electrochemical gradient Chloride channels Thermospermine P33 Soil chemistry and physics F60 Plant physiology and biochemistry Diospyros lotus Salt stress Gil-Muñoz, Francisco Delhomme, Nicolas Quinones, Ana Naval, María M. Badenes, María L. García-Gil, M. Rosario Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title | Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title_full | Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title_fullStr | Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title_full_unstemmed | Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title_short | Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros lotus L.) |
| title_sort | transcriptomic analysis reveals salt tolerance mechanisms present in date plum persimmon rootstock diospyros lotus l |
| topic | Ion transport Persimmon Transmembrane electrochemical gradient Chloride channels Thermospermine P33 Soil chemistry and physics F60 Plant physiology and biochemistry Diospyros lotus Salt stress |
| url | http://hdl.handle.net/20.500.11939/6966 https://www.mdpi.com/2073-4395/10/11/1703 |
| work_keys_str_mv | AT gilmunozfrancisco transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl AT delhommenicolas transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl AT quinonesana transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl AT navalmariam transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl AT badenesmarial transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl AT garciagilmrosario transcriptomicanalysisrevealssalttolerancemechanismspresentindateplumpersimmonrootstockdiospyroslotusl |