The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation
Damage caused by Citrus psorosis virus (CPsV) could be avoided obtaining resistant cultivars by breeding, but the sensitivity of most citrus hosts to CPsV is unknown. To find potential sources of resistance we inoculated 63 cultivars and hybrids of Citrus and related genera [Citrus (36), Microc...
| Main Authors: | , , , , , , , , |
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| Format: | article |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/6937 https://link.springer.com/article/10.1007/s10658-015-0751-3 |
| _version_ | 1855032517430607872 |
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| author | Velázquez, Karelia Alba, Lorena Zarza, Óscar Vives, María C. Pina, José A. Juárez, José Navarro, Luis Moreno, Pedro Guerri, José |
| author_browse | Alba, Lorena Guerri, José Juárez, José Moreno, Pedro Navarro, Luis Pina, José A. Velázquez, Karelia Vives, María C. Zarza, Óscar |
| author_facet | Velázquez, Karelia Alba, Lorena Zarza, Óscar Vives, María C. Pina, José A. Juárez, José Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Velázquez, Karelia |
| collection | ReDivia |
| description | Damage caused by Citrus psorosis virus
(CPsV) could be avoided obtaining resistant cultivars
by breeding, but the sensitivity of most citrus hosts to
CPsV is unknown. To find potential sources of resistance
we inoculated 63 cultivars and hybrids of Citrus
and related genera [Citrus (36), Microcitrus (5),
Fortunella (6), Eremocitrus (1), Pleiospermium (1),
Atalantia (2), Severinia (1), Clausena (1), Swinglea
(1), Afraegle (1), Poncirus (1) and hybrids (7)] with
the CPsV isolate PB-143 and monitored symptoms
and CPsV infection by ELISA. Microcitrus inodora
and Fortunella hindsii were symptomless but gave high
ELISA values, suggesting tolerance to CPsV, whereas
Citrus depresa, Clausena excavata, Cleopatra mandarin,
sour orange, Carrizo citrange and CPB4475
citrumelo were ELISA negative, suggesting resistance.
Further examination of CPsV infection by reverse transcription
quantitative real time PCR (RT-qPCR) in
Cleopatra mandarin, Poncirus trifoliata and Carrizo
citrange seedlings inoculated with CPsV isolates
P-121, PB-102 and PB-143, revealed different infection
rates and CPsV accumulation, depending on the isolate and citrus genotype. Bud propagation of these genotypes
on CPsV-inoculated sweet orange plants incited
a bud union disorder that hindered flushing and scion
growth, suggesting that the high viral load in the rootstock
induced a hypersensitive-like reaction in the partially
resistant scion. |
| format | article |
| id | ReDivia6937 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia69372025-04-25T14:47:58Z The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation Velázquez, Karelia Alba, Lorena Zarza, Óscar Vives, María C. Pina, José A. Juárez, José Navarro, Luis Moreno, Pedro Guerri, José Citrus psorosis disease RT-qPCR Bud union disorder Citrus species and hybrids Citrus relatives H20 Plant diseases Damage caused by Citrus psorosis virus (CPsV) could be avoided obtaining resistant cultivars by breeding, but the sensitivity of most citrus hosts to CPsV is unknown. To find potential sources of resistance we inoculated 63 cultivars and hybrids of Citrus and related genera [Citrus (36), Microcitrus (5), Fortunella (6), Eremocitrus (1), Pleiospermium (1), Atalantia (2), Severinia (1), Clausena (1), Swinglea (1), Afraegle (1), Poncirus (1) and hybrids (7)] with the CPsV isolate PB-143 and monitored symptoms and CPsV infection by ELISA. Microcitrus inodora and Fortunella hindsii were symptomless but gave high ELISA values, suggesting tolerance to CPsV, whereas Citrus depresa, Clausena excavata, Cleopatra mandarin, sour orange, Carrizo citrange and CPB4475 citrumelo were ELISA negative, suggesting resistance. Further examination of CPsV infection by reverse transcription quantitative real time PCR (RT-qPCR) in Cleopatra mandarin, Poncirus trifoliata and Carrizo citrange seedlings inoculated with CPsV isolates P-121, PB-102 and PB-143, revealed different infection rates and CPsV accumulation, depending on the isolate and citrus genotype. Bud propagation of these genotypes on CPsV-inoculated sweet orange plants incited a bud union disorder that hindered flushing and scion growth, suggesting that the high viral load in the rootstock induced a hypersensitive-like reaction in the partially resistant scion. 2021-01-11T14:00:10Z 2021-01-11T14:00:10Z 2016 article publishedVersion Velázquez, K., Alba, L., Zarza, O., Vives, M. C., Pina, J. A., Juárez, J., ... & Guerri, J. (2016). The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation. European Journal of Plant Pathology, 144(1), 73-81. 0929-1873 (Print ISSN) 1573-8469 (Electronic ISSN) http://hdl.handle.net/20.500.11939/6937 10.1007/s10658-015-0751-3 https://link.springer.com/article/10.1007/s10658-015-0751-3 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Springer electronico |
| spellingShingle | Citrus psorosis disease RT-qPCR Bud union disorder Citrus species and hybrids Citrus relatives H20 Plant diseases Velázquez, Karelia Alba, Lorena Zarza, Óscar Vives, María C. Pina, José A. Juárez, José Navarro, Luis Moreno, Pedro Guerri, José The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title | The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title_full | The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title_fullStr | The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title_full_unstemmed | The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title_short | The response of different genotypes of citrus and relatives to Citrus psorosis virus inoculation |
| title_sort | response of different genotypes of citrus and relatives to citrus psorosis virus inoculation |
| topic | Citrus psorosis disease RT-qPCR Bud union disorder Citrus species and hybrids Citrus relatives H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6937 https://link.springer.com/article/10.1007/s10658-015-0751-3 |
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