Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity
Pomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subject...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6934 https://www.mdpi.com/2073-4395/10/11/1821 |
| _version_ | 1855032516907368448 |
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| author | Caglayan, Kadriye Gazel, Mona Roumi, Vahid Kocabag, Hamide Deniz Bahar, Tunç Reynard, Jean Sebastien Ruiz-García, Ana B. Olmos, Antonio Candresse, Thierry |
| author_browse | Bahar, Tunç Caglayan, Kadriye Candresse, Thierry Gazel, Mona Kocabag, Hamide Deniz Olmos, Antonio Reynard, Jean Sebastien Roumi, Vahid Ruiz-García, Ana B. |
| author_facet | Caglayan, Kadriye Gazel, Mona Roumi, Vahid Kocabag, Hamide Deniz Bahar, Tunç Reynard, Jean Sebastien Ruiz-García, Ana B. Olmos, Antonio Candresse, Thierry |
| author_sort | Caglayan, Kadriye |
| collection | ReDivia |
| description | Pomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0–86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV |
| format | article |
| id | ReDivia6934 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia69342025-04-25T14:47:57Z Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity Caglayan, Kadriye Gazel, Mona Roumi, Vahid Kocabag, Hamide Deniz Bahar, Tunç Reynard, Jean Sebastien Ruiz-García, Ana B. Olmos, Antonio Candresse, Thierry Potyviridae HTS RT-PCR H20 Plant diseases Punica granatum Electron microscopy Pomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0–86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV 2021-01-11T13:58:07Z 2021-01-11T13:58:07Z 2020 article publishedVersion Caglayan, K.; Gazel, M.; Roumi, V.; Kocabag, H.D.; Tunç, B.; Reynard, J.S.; Ruiz-García, A.B.; Olmos, A. & Candresse, T. (2020). Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity. Agronomy 2020, 10, 1821 2073-4395 http://hdl.handle.net/20.500.11939/6934 10.3390/agronomy10111821 https://www.mdpi.com/2073-4395/10/11/1821 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Potyviridae HTS RT-PCR H20 Plant diseases Punica granatum Electron microscopy Caglayan, Kadriye Gazel, Mona Roumi, Vahid Kocabag, Hamide Deniz Bahar, Tunç Reynard, Jean Sebastien Ruiz-García, Ana B. Olmos, Antonio Candresse, Thierry Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title | Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title_full | Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title_fullStr | Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title_full_unstemmed | Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title_short | Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity |
| title_sort | identification of pomegranate as a new host of passiflora edulis symptomless virus pesv and analysis of pesv diversity |
| topic | Potyviridae HTS RT-PCR H20 Plant diseases Punica granatum Electron microscopy |
| url | http://hdl.handle.net/20.500.11939/6934 https://www.mdpi.com/2073-4395/10/11/1821 |
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