Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species

Citrus is a wide genus in which most of the cultivated species and cultivars are natural parthenocarpic mutants or hybrids (i.e. orange, mandarin, tangerine, grapefruit). The autonomous increase in GA1 ovary concentration during anthesis was suggested as being the stimulus responsible for parthenoca...

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Autores principales: Mesejo, Carlos, Yuste, Roberto, Reig, Carmina, Martínez-Fuentes, Amparo, Iglesias, Domingo J., Munoz-Fambuena, Natalia, Bermejo, Almudena, Germana, Maria Antonietta, Primo-Millo, Eduardo, Agustí, Manuel
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/6913
https://www.sciencedirect.com/science/article/abs/pii/S0168945216300309#!
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author Mesejo, Carlos
Yuste, Roberto
Reig, Carmina
Martínez-Fuentes, Amparo
Iglesias, Domingo J.
Munoz-Fambuena, Natalia
Bermejo, Almudena
Germana, Maria Antonietta
Primo-Millo, Eduardo
Agustí, Manuel
author_browse Agustí, Manuel
Bermejo, Almudena
Germana, Maria Antonietta
Iglesias, Domingo J.
Martínez-Fuentes, Amparo
Mesejo, Carlos
Munoz-Fambuena, Natalia
Primo-Millo, Eduardo
Reig, Carmina
Yuste, Roberto
author_facet Mesejo, Carlos
Yuste, Roberto
Reig, Carmina
Martínez-Fuentes, Amparo
Iglesias, Domingo J.
Munoz-Fambuena, Natalia
Bermejo, Almudena
Germana, Maria Antonietta
Primo-Millo, Eduardo
Agustí, Manuel
author_sort Mesejo, Carlos
collection ReDivia
description Citrus is a wide genus in which most of the cultivated species and cultivars are natural parthenocarpic mutants or hybrids (i.e. orange, mandarin, tangerine, grapefruit). The autonomous increase in GA1 ovary concentration during anthesis was suggested as being the stimulus responsible for parthenocarpy in Citrus regardless of the species. To determine the exact GA-role in parthenocarpic fruit set, the following hypothesis was tested: GA triggers and maintains cell division in ovary walls causing fruit set. Obligate and facultative parthenocarpic Citrus species were used as a model system because obligate parthenocarpic Citrus sp (i.e. Citrus unshiu) have higher GA levels and better natural parthenocarpic fruit set compared to other facultative parthenocarpic Citrus (i.e. Citrus clementina). The autonomous activation of GA synthesis in C. unshiu ovary preceded cell division and CYCA1.1 up-regulation (a G2-stage cell cycle regulator) at anthesis setting a high proportion of fruits, whereas C. clementina lacked this GA-biosynthesis and CYCA1.1 up-regulation failing in fruit set. In situ hybridization experiments revealed a tissue-specific expression of GA20ox2 only in the dividing tissues of the pericarp. Furthermore, CYCA1.1 expression correlated endogenous GA1 content with GA3 treatment, which stimulated cell division and ovary growth, mostly in C. clementina. Instead, paclobutrazol (GA biosynthesis inhibitor) negated cell division and reduced fruit set. Results suggest that in parthenocarpic citrus the specific GA synthesis in the ovary walls at anthesis triggers cell division and, thus, the necessary ovary growth rate to set fruit.
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spelling ReDivia69132025-04-25T14:47:56Z Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species Mesejo, Carlos Yuste, Roberto Reig, Carmina Martínez-Fuentes, Amparo Iglesias, Domingo J. Munoz-Fambuena, Natalia Bermejo, Almudena Germana, Maria Antonietta Primo-Millo, Eduardo Agustí, Manuel F63 Plant physiology - Reproduction F60 Plant physiology and biochemistry Citrus Flowers Fruit set Fruit trees Gibberellins Parthenocarpy Citrus is a wide genus in which most of the cultivated species and cultivars are natural parthenocarpic mutants or hybrids (i.e. orange, mandarin, tangerine, grapefruit). The autonomous increase in GA1 ovary concentration during anthesis was suggested as being the stimulus responsible for parthenocarpy in Citrus regardless of the species. To determine the exact GA-role in parthenocarpic fruit set, the following hypothesis was tested: GA triggers and maintains cell division in ovary walls causing fruit set. Obligate and facultative parthenocarpic Citrus species were used as a model system because obligate parthenocarpic Citrus sp (i.e. Citrus unshiu) have higher GA levels and better natural parthenocarpic fruit set compared to other facultative parthenocarpic Citrus (i.e. Citrus clementina). The autonomous activation of GA synthesis in C. unshiu ovary preceded cell division and CYCA1.1 up-regulation (a G2-stage cell cycle regulator) at anthesis setting a high proportion of fruits, whereas C. clementina lacked this GA-biosynthesis and CYCA1.1 up-regulation failing in fruit set. In situ hybridization experiments revealed a tissue-specific expression of GA20ox2 only in the dividing tissues of the pericarp. Furthermore, CYCA1.1 expression correlated endogenous GA1 content with GA3 treatment, which stimulated cell division and ovary growth, mostly in C. clementina. Instead, paclobutrazol (GA biosynthesis inhibitor) negated cell division and reduced fruit set. Results suggest that in parthenocarpic citrus the specific GA synthesis in the ovary walls at anthesis triggers cell division and, thus, the necessary ovary growth rate to set fruit. 2020-12-23T11:52:57Z 2020-12-23T11:52:57Z 2016 article publishedVersion Mesejo, C., Yuste, R., Reig, C., Martínez-Fuentes, A., Iglesias, D. J., Muñoz-Fambuena, N. et al. (2016). Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species. Plant Science, 247, 13-24. 0168-9452 http://hdl.handle.net/20.500.11939/6913 10.1016/j.plantsci.2016.02.018 https://www.sciencedirect.com/science/article/abs/pii/S0168945216300309#! en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico
spellingShingle F63 Plant physiology - Reproduction
F60 Plant physiology and biochemistry
Citrus
Flowers
Fruit set
Fruit trees
Gibberellins
Parthenocarpy
Mesejo, Carlos
Yuste, Roberto
Reig, Carmina
Martínez-Fuentes, Amparo
Iglesias, Domingo J.
Munoz-Fambuena, Natalia
Bermejo, Almudena
Germana, Maria Antonietta
Primo-Millo, Eduardo
Agustí, Manuel
Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title_full Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title_fullStr Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title_full_unstemmed Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title_short Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species
title_sort gibberellin reactivates and maintains ovary wall cell division causing fruit set in parthenocarpic citrus species
topic F63 Plant physiology - Reproduction
F60 Plant physiology and biochemistry
Citrus
Flowers
Fruit set
Fruit trees
Gibberellins
Parthenocarpy
url http://hdl.handle.net/20.500.11939/6913
https://www.sciencedirect.com/science/article/abs/pii/S0168945216300309#!
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