A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4

MYB-bHLH-WD40 (MBW) ternary complexes composed of MYB domain proteins, basic helix-loop-helix (bHLH) transcription factors and WD40-repeat proteins are involved in the transcriptional regulation of anthocyanin and proanthocyanidin (PA) biosynthesis pathways in distinct plant species. PAs are the co...

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Autores principales: Naval, María M., Gil-Muñoz, Francisco, Lloret, Alba, Besada, Cristina, Salvador, Alejandra, Badenes, María L., Ríos, Gabino
Formato: article
Lenguaje:Inglés
Publicado: Springer 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/6877
https://link.springer.com/article/10.1007/s11295-016-0969-z
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author Naval, María M.
Gil-Muñoz, Francisco
Lloret, Alba
Besada, Cristina
Salvador, Alejandra
Badenes, María L.
Ríos, Gabino
author_browse Badenes, María L.
Besada, Cristina
Gil-Muñoz, Francisco
Lloret, Alba
Naval, María M.
Ríos, Gabino
Salvador, Alejandra
author_facet Naval, María M.
Gil-Muñoz, Francisco
Lloret, Alba
Besada, Cristina
Salvador, Alejandra
Badenes, María L.
Ríos, Gabino
author_sort Naval, María M.
collection ReDivia
description MYB-bHLH-WD40 (MBW) ternary complexes composed of MYB domain proteins, basic helix-loop-helix (bHLH) transcription factors and WD40-repeat proteins are involved in the transcriptional regulation of anthocyanin and proanthocyanidin (PA) biosynthesis pathways in distinct plant species. PAs are the compounds responsible for the strong astringent taste of persimmon fruit. DkMYB2 and DkMYB4 (MYB type) and DkMYC1 (bHLH type) genes from persimmon have been postulated to regulate the biosynthesis of PAs in fruit.We have identified a WD40-repeat gene from persimmon (DkWDR1) coding for a protein highly similar to TTG1 from Arabidopsis thaliana and other WD40-repeat components of distinct MBW complexes. DkWDR1 expression was detected in different tissues and was essentially constant during fruit development. MYB-like proteins DkMYB2 and DkMYB4 interacted with both DkWDR1 and DkMYC1 in the yeast two-hybrid system. The sequence of DkWDR1 and the analysis of yeast two-hybrid interactions with known regulators of PA biosynthesis support the occurrence of MYBbHLH-WD40 (MBW) complexes in persimmon, to regulate PA accumulation in fruit.
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spelling ReDivia68772025-04-25T14:47:55Z A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4 Naval, María M. Gil-Muñoz, Francisco Lloret, Alba Besada, Cristina Salvador, Alejandra Badenes, María L. Ríos, Gabino Fruit astringency MBW complex WD40 repeat Protein interaction F60 Plant physiology and biochemistry Q01 Food science and technology Diospyros kaki Tannins MYB-bHLH-WD40 (MBW) ternary complexes composed of MYB domain proteins, basic helix-loop-helix (bHLH) transcription factors and WD40-repeat proteins are involved in the transcriptional regulation of anthocyanin and proanthocyanidin (PA) biosynthesis pathways in distinct plant species. PAs are the compounds responsible for the strong astringent taste of persimmon fruit. DkMYB2 and DkMYB4 (MYB type) and DkMYC1 (bHLH type) genes from persimmon have been postulated to regulate the biosynthesis of PAs in fruit.We have identified a WD40-repeat gene from persimmon (DkWDR1) coding for a protein highly similar to TTG1 from Arabidopsis thaliana and other WD40-repeat components of distinct MBW complexes. DkWDR1 expression was detected in different tissues and was essentially constant during fruit development. MYB-like proteins DkMYB2 and DkMYB4 interacted with both DkWDR1 and DkMYC1 in the yeast two-hybrid system. The sequence of DkWDR1 and the analysis of yeast two-hybrid interactions with known regulators of PA biosynthesis support the occurrence of MYBbHLH-WD40 (MBW) complexes in persimmon, to regulate PA accumulation in fruit. 2020-12-11T08:37:09Z 2020-12-11T08:37:09Z 2016 article publishedVersion Naval, M. M., Gil-Muñoz, F., Lloret, A., Besada, C., Salvador, A., Badenes, M. L. et al. (2016). A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4. Tree Genetics & Genomes, 12, 13 1614-2950 (e-ISSN) http://hdl.handle.net/20.500.11939/6877 10.1007/s11295-016-0969-z https://link.springer.com/article/10.1007/s11295-016-0969-z en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Springer electronico
spellingShingle Fruit astringency
MBW complex
WD40 repeat
Protein interaction
F60 Plant physiology and biochemistry
Q01 Food science and technology
Diospyros kaki
Tannins
Naval, María M.
Gil-Muñoz, Francisco
Lloret, Alba
Besada, Cristina
Salvador, Alejandra
Badenes, María L.
Ríos, Gabino
A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title_full A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title_fullStr A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title_full_unstemmed A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title_short A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4
title_sort wd40 repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis dkmyb2 and dkmyb4
topic Fruit astringency
MBW complex
WD40 repeat
Protein interaction
F60 Plant physiology and biochemistry
Q01 Food science and technology
Diospyros kaki
Tannins
url http://hdl.handle.net/20.500.11939/6877
https://link.springer.com/article/10.1007/s11295-016-0969-z
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