Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing
Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive single-stranded RNA (+ssRNA) virus infecting important horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in virus replication, whereas RNA2 encodes the large and small coat p...
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| Format: | article |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6874 https://link.springer.com/article/10.1007/s10658-020-02117-3 |
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| author | Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis |
| author_browse | Carpino, Caterina Davino, Salvatore Elvira-González, Laura Ferriol, Inmaculada Galipienso, Luis Peri, Ezio Rubio, Luis |
| author_facet | Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis |
| author_sort | Carpino, Caterina |
| collection | ReDivia |
| description | Broad bean wilt virus 1 (BBWV-1, genus
Fabavirus, family Secoviridae) is a bipartite positive
single-stranded RNA (+ssRNA) virus infecting important
horticultural and ornamental crops worldwide.
RNA1 encodes proteins involved in virus replication,
whereas RNA2 encodes the large and small coat proteins
(LCP, and SCP, respectively) and two putative
movement proteins with overlapping C-terminal but
different sizes: 47.2 kDa (VP47) and 37 kDa (VP37).
Post-transcriptional gene silencing (PTGS) is a mechanism
of gene regulation and defense against pathogens
such as viruses. However, most plant viruses encode
proteins called viral suppressors of RNA silencing
(VSRs) which able to inhibit PTGS. Previously,
BBWV-1 VP37 was identified as VSR and here, we
have demonstrated that VP47 and SCP, but not LCP, are
also VSRs by two approaches: (i) transient expression in
Nicotiana benthamiana 16c plants expressing the green
fluorescent protein (GFP) constitutively, and (ii) movement
complementation assays using a viral vector based
on Turnip crinkle virus sequence containing GFP
(pTCV-GFP). In contrast LCP but not SCP of Broad
been wilt virus 2 (BBWV-2, genus Fabavirus, family
Secoviridae) is a VSR, which illustrates that viruses of
the same genus can interact differently with the host. |
| format | article |
| id | ReDivia6874 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia68742025-04-25T14:47:54Z Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis Secoviridae BBWV-1 Fabavirus Silencing suppressor H20 Plant diseases PTGS Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive single-stranded RNA (+ssRNA) virus infecting important horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in virus replication, whereas RNA2 encodes the large and small coat proteins (LCP, and SCP, respectively) and two putative movement proteins with overlapping C-terminal but different sizes: 47.2 kDa (VP47) and 37 kDa (VP37). Post-transcriptional gene silencing (PTGS) is a mechanism of gene regulation and defense against pathogens such as viruses. However, most plant viruses encode proteins called viral suppressors of RNA silencing (VSRs) which able to inhibit PTGS. Previously, BBWV-1 VP37 was identified as VSR and here, we have demonstrated that VP47 and SCP, but not LCP, are also VSRs by two approaches: (i) transient expression in Nicotiana benthamiana 16c plants expressing the green fluorescent protein (GFP) constitutively, and (ii) movement complementation assays using a viral vector based on Turnip crinkle virus sequence containing GFP (pTCV-GFP). In contrast LCP but not SCP of Broad been wilt virus 2 (BBWV-2, genus Fabavirus, family Secoviridae) is a VSR, which illustrates that viruses of the same genus can interact differently with the host. 2020-12-09T10:03:29Z 2020-12-09T10:03:29Z 2020 article publishedVersion Carpino, C., Ferriol, I., Elvira-González, L. et al. (2020). Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing. Eur J Plant Pathol 158, 1043–1049 0929-1873 (Print ISSN) 1573-8469 (Electronic ISSN) http://hdl.handle.net/20.500.11939/6874 10.1007/s10658-020-02117-3 https://link.springer.com/article/10.1007/s10658-020-02117-3 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Springer electronico |
| spellingShingle | Secoviridae BBWV-1 Fabavirus Silencing suppressor H20 Plant diseases PTGS Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title | Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title_full | Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title_fullStr | Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title_full_unstemmed | Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title_short | Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing |
| title_sort | broad bean wilt virus 1 encoded vp47 and scp are suppressors of plant post transcriptional gene silencing |
| topic | Secoviridae BBWV-1 Fabavirus Silencing suppressor H20 Plant diseases PTGS |
| url | http://hdl.handle.net/20.500.11939/6874 https://link.springer.com/article/10.1007/s10658-020-02117-3 |
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