Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus
Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P < 0.01) expression chang...
| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6856 https://www.sciencedirect.com/science/article/pii/S0042682207003807 |
| _version_ | 1855032502752641024 |
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| author | Gandía, Mónica Conesa, Ana Ancillo, Gema Gadea, Jose Forment, Javier Pallás, Vicente Flores, Ricardo Durán-Vila, Núria Moreno, Pedro Guerri, José |
| author_browse | Ancillo, Gema Conesa, Ana Durán-Vila, Núria Flores, Ricardo Forment, Javier Gadea, Jose Gandía, Mónica Guerri, José Moreno, Pedro Pallás, Vicente |
| author_facet | Gandía, Mónica Conesa, Ana Ancillo, Gema Gadea, Jose Forment, Javier Pallás, Vicente Flores, Ricardo Durán-Vila, Núria Moreno, Pedro Guerri, José |
| author_sort | Gandía, Mónica |
| collection | ReDivia |
| description | Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P < 0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress. |
| format | article |
| id | ReDivia6856 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia68562025-04-25T14:47:53Z Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus Gandía, Mónica Conesa, Ana Ancillo, Gema Gadea, Jose Forment, Javier Pallás, Vicente Flores, Ricardo Durán-Vila, Núria Moreno, Pedro Guerri, José Mexican lime Microarray Symptom expression H20 Plant diseases F30 Plant genetics and breeding Citrus tristeza virus Defense response Gene expression Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P < 0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress. 2020-12-03T09:28:35Z 2020-12-03T09:28:35Z 2007 article publishedVersion Gandía, M., Conesa, A., Ancillo, G., Gadea, J., Forment, J., Pallás, V. et al. (2007). Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus. Virology, 367(2), 298-306. 0042-6822 http://hdl.handle.net/20.500.11939/6856 10.1016/j.virol.2007.05.025 https://www.sciencedirect.com/science/article/pii/S0042682207003807 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Mexican lime Microarray Symptom expression H20 Plant diseases F30 Plant genetics and breeding Citrus tristeza virus Defense response Gene expression Gandía, Mónica Conesa, Ana Ancillo, Gema Gadea, Jose Forment, Javier Pallás, Vicente Flores, Ricardo Durán-Vila, Núria Moreno, Pedro Guerri, José Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title | Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title_full | Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title_fullStr | Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title_full_unstemmed | Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title_short | Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus |
| title_sort | transcriptional response of citrus aurantifolia to infection by citrus tristeza virus |
| topic | Mexican lime Microarray Symptom expression H20 Plant diseases F30 Plant genetics and breeding Citrus tristeza virus Defense response Gene expression |
| url | http://hdl.handle.net/20.500.11939/6856 https://www.sciencedirect.com/science/article/pii/S0042682207003807 |
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