Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus
Loquat (Eriobotrya japonica) is a minor but important woody crop cultivated in Asia and Europe. High-throughput sequencing (HTS) analysis of an asymptomatic loquat plant using RNAseq Illumina technology has allowed the detection for the first time of apple stem pitting virus (ASPV), the type species...
| Autores principales: | , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
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MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6844 https://www.mdpi.com/2223-7747/9/11/1560 |
| _version_ | 1855032500539097088 |
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| author | Morán, Félix Canales, Celia Olmos, Antonio Ruiz-García, Ana B. |
| author_browse | Canales, Celia Morán, Félix Olmos, Antonio Ruiz-García, Ana B. |
| author_facet | Morán, Félix Canales, Celia Olmos, Antonio Ruiz-García, Ana B. |
| author_sort | Morán, Félix |
| collection | ReDivia |
| description | Loquat (Eriobotrya japonica) is a minor but important woody crop cultivated in Asia and Europe. High-throughput sequencing (HTS) analysis of an asymptomatic loquat plant using RNAseq Illumina technology has allowed the detection for the first time of apple stem pitting virus (ASPV), the type species of the genus Foveavirus in the family Betaflexiviridae, infecting this crop. A nearly complete genome of 9303 nts (ASPV-SL61) reconstructed bioinformatically shows the typical genomic structure of this viral species and a highest nucleotide identity (85.9%) with the Chinese ASPV isolate YLX from pear. A close phylogenetic relationship between ASPV-SL61 and ASPV-YLX has been confirmed by the sequence analysis of full-length ASPV genomic sequences available in the databases. In fact, a phylogenetic study based on a partial CP N-terminal sequence previously proposed to be involved in host adaptation has shown that ASPV-SL61 loquat isolate is more closely related to ASPV pear isolates. The presence of ASPV in loquat has been further confirmed by RT-PCR and Sanger sequencing and DAS-ELISA. An incidence of 15% was determined in one of the loquat Spanish growing areas. The sequence analysis of the partial CP sequences amplified by RT-PCR has shown a high level of variability between loquat isolates. To our knowledge, this is the first record of loquat as a natural host of ASPV. |
| format | publishedVersion |
| id | ReDivia6844 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia68442025-04-25T14:47:53Z Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus Morán, Félix Canales, Celia Olmos, Antonio Ruiz-García, Ana B. ASPV HTS loquat H20 Plant diseases Foveavirus Betaflexiviridae Loquat (Eriobotrya japonica) is a minor but important woody crop cultivated in Asia and Europe. High-throughput sequencing (HTS) analysis of an asymptomatic loquat plant using RNAseq Illumina technology has allowed the detection for the first time of apple stem pitting virus (ASPV), the type species of the genus Foveavirus in the family Betaflexiviridae, infecting this crop. A nearly complete genome of 9303 nts (ASPV-SL61) reconstructed bioinformatically shows the typical genomic structure of this viral species and a highest nucleotide identity (85.9%) with the Chinese ASPV isolate YLX from pear. A close phylogenetic relationship between ASPV-SL61 and ASPV-YLX has been confirmed by the sequence analysis of full-length ASPV genomic sequences available in the databases. In fact, a phylogenetic study based on a partial CP N-terminal sequence previously proposed to be involved in host adaptation has shown that ASPV-SL61 loquat isolate is more closely related to ASPV pear isolates. The presence of ASPV in loquat has been further confirmed by RT-PCR and Sanger sequencing and DAS-ELISA. An incidence of 15% was determined in one of the loquat Spanish growing areas. The sequence analysis of the partial CP sequences amplified by RT-PCR has shown a high level of variability between loquat isolates. To our knowledge, this is the first record of loquat as a natural host of ASPV. 2020-11-30T11:44:58Z 2020-11-30T11:44:58Z 2020 publishedVersion Morán, F.; Canales, C.; Olmos, A.; Ruiz-García, A.B. Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus. Plants 2020, 9, 1560. 2223-7747 http://hdl.handle.net/20.500.11939/6844 10.3390/plants9111560 https://www.mdpi.com/2223-7747/9/11/1560 en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/E-RTA2017-00009 info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51912 Instituto Nacional de Investigaciones Agrarias (INIA) E-RTA2017-00009 Instituto Valenciano de Investigaciones Agrarias (IVIA) 51912 grant. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | ASPV HTS loquat H20 Plant diseases Foveavirus Betaflexiviridae Morán, Félix Canales, Celia Olmos, Antonio Ruiz-García, Ana B. Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title | Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title_full | Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title_fullStr | Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title_full_unstemmed | Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title_short | Loquat (Eriobotrya japonica) is a New Natural Host of Apple Stem Pitting Virus |
| title_sort | loquat eriobotrya japonica is a new natural host of apple stem pitting virus |
| topic | ASPV HTS loquat H20 Plant diseases Foveavirus Betaflexiviridae |
| url | http://hdl.handle.net/20.500.11939/6844 https://www.mdpi.com/2223-7747/9/11/1560 |
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