Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting
Somatic embryogenesis and plant regeneration are basic processes for the success of citrus somatic hybridization via protoplast fusion. In many cases, few embryos develop normally and only a small number of plants are recovered. The development of methodologies able to increase the recovery of plant...
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| Format: | article |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6840 https://link.springer.com/article/10.1007/s11240-016-0991-8 |
| _version_ | 1855032499791462400 |
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| author | Aleza, Pablo García-Lor, Andrés Juárez, José Navarro, Luis |
| author_browse | Aleza, Pablo García-Lor, Andrés Juárez, José Navarro, Luis |
| author_facet | Aleza, Pablo García-Lor, Andrés Juárez, José Navarro, Luis |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Somatic embryogenesis and plant regeneration are basic processes for the success of citrus somatic hybridization via protoplast fusion. In many cases, few embryos develop normally and only a small number of plants are recovered. The development of methodologies able to increase the recovery of plants after protoplast fusion experiments it is an important requirement to improve the efficiency of the procedure. Here, plants were regenerated at high efficiency using in vitro micrografting of shoots, roots, and embryos recovered after different somatic hybridizations. Hybridizations were performed using protoplasts isolated from Chios mandarin callus with protoplasts isolated from Clementine mandarin leaves and from Sanguinelli sweet orange callus. Recovered plants were analyzed with flow cytometry and nuclear simple sequence repeat (SSR), mitochondrial InDel, and chloroplast SSR markers to determine genomic structure. One tetraploid cybrid and numerous diploid cybrids were recovered, and these exhibited a range of mitochondrial and chloroplastic genome combinations. |
| format | article |
| id | ReDivia6840 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia68402025-04-25T14:47:52Z Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting Aleza, Pablo García-Lor, Andrés Juárez, José Navarro, Luis SSR markers F30 Plant genetics and breeding Mandarins Breeding Somatic hybridization Flow cytometry (cells) Tetraploids Somatic embryogenesis and plant regeneration are basic processes for the success of citrus somatic hybridization via protoplast fusion. In many cases, few embryos develop normally and only a small number of plants are recovered. The development of methodologies able to increase the recovery of plants after protoplast fusion experiments it is an important requirement to improve the efficiency of the procedure. Here, plants were regenerated at high efficiency using in vitro micrografting of shoots, roots, and embryos recovered after different somatic hybridizations. Hybridizations were performed using protoplasts isolated from Chios mandarin callus with protoplasts isolated from Clementine mandarin leaves and from Sanguinelli sweet orange callus. Recovered plants were analyzed with flow cytometry and nuclear simple sequence repeat (SSR), mitochondrial InDel, and chloroplast SSR markers to determine genomic structure. One tetraploid cybrid and numerous diploid cybrids were recovered, and these exhibited a range of mitochondrial and chloroplastic genome combinations. 2020-11-27T09:55:13Z 2020-11-27T09:55:13Z 2016 article publishedVersion Aleza, P., Garcia-Lor, A., Juárez, J., & Navarro, L. (2016). Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting. Plant Cell, Tissue and Organ Culture (PCTOC), 126(2), 205-217. 0167-6857 (Print ISSN) 1573-5044 (Online ISSN) http://hdl.handle.net/20.500.11939/6840 10.1007/s11240-016-0991-8 https://link.springer.com/article/10.1007/s11240-016-0991-8 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Springer electronico |
| spellingShingle | SSR markers F30 Plant genetics and breeding Mandarins Breeding Somatic hybridization Flow cytometry (cells) Tetraploids Aleza, Pablo García-Lor, Andrés Juárez, José Navarro, Luis Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title | Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title_full | Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title_fullStr | Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title_full_unstemmed | Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title_short | Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting |
| title_sort | recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot root or embryo micrografting |
| topic | SSR markers F30 Plant genetics and breeding Mandarins Breeding Somatic hybridization Flow cytometry (cells) Tetraploids |
| url | http://hdl.handle.net/20.500.11939/6840 https://link.springer.com/article/10.1007/s11240-016-0991-8 |
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