Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection
Green mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showe...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6838 https://www.sciencedirect.com/science/article/abs/pii/S0168160516303816 |
| _version_ | 1855492160982351872 |
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| author | Vilanova, Laura Teixidó, Neus Torres, Rosario Usall, Josep Vinas, Inmaculada Sánchez-Torres, Paloma |
| author_browse | Sánchez-Torres, Paloma Teixidó, Neus Torres, Rosario Usall, Josep Vilanova, Laura Vinas, Inmaculada |
| author_facet | Vilanova, Laura Teixidó, Neus Torres, Rosario Usall, Josep Vinas, Inmaculada Sánchez-Torres, Paloma |
| author_sort | Vilanova, Laura |
| collection | ReDivia |
| description | Green mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showed significantly reduced virulence during citrus fruit infection. Virulence was affected not only in mature fruit but also in immature fruit, and disease severity was markedly reduced when the oranges were stored at 20 or 4 °C. In addition, the ΔPdSte12 mutants were defective in asexual reproduction, producing few conidia. The conidiophores of these mutants had longer metulae with fewer branches at the tip of the hyphae. Gene expression analysis revealed that PdSte12 might act as a negative regulator of several transporter-encoding genes and a positive regulator of two sterol demethylases, all of which are involved in fungicide resistance and fungal virulence. Moreover, PdSte12 exhibited the negative regulation of another transcription factor PdMut3, putatively involved in fungal pathogenesis but with no effect on the MAPK SLT2 P. digitatum orthologue belonging to different transcription pathways relevant to cell integrity. These results indicate the PdSte12 transcription factor is functionally conserved in P. digitatum for infection and asexual reproduction, similar to other Ste12 fungal plant pathogens. |
| format | Artículo |
| id | ReDivia6838 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia68382025-04-25T14:47:52Z Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection Vilanova, Laura Teixidó, Neus Torres, Rosario Usall, Josep Vinas, Inmaculada Sánchez-Torres, Paloma Conidiation Fruit-pathogen interaction Q02 Food processing and preservation J10 Handling, transport, storage and protection of agricultural products Virulence Transcription factors Penicillium digitatum Citrus Green mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showed significantly reduced virulence during citrus fruit infection. Virulence was affected not only in mature fruit but also in immature fruit, and disease severity was markedly reduced when the oranges were stored at 20 or 4 °C. In addition, the ΔPdSte12 mutants were defective in asexual reproduction, producing few conidia. The conidiophores of these mutants had longer metulae with fewer branches at the tip of the hyphae. Gene expression analysis revealed that PdSte12 might act as a negative regulator of several transporter-encoding genes and a positive regulator of two sterol demethylases, all of which are involved in fungicide resistance and fungal virulence. Moreover, PdSte12 exhibited the negative regulation of another transcription factor PdMut3, putatively involved in fungal pathogenesis but with no effect on the MAPK SLT2 P. digitatum orthologue belonging to different transcription pathways relevant to cell integrity. These results indicate the PdSte12 transcription factor is functionally conserved in P. digitatum for infection and asexual reproduction, similar to other Ste12 fungal plant pathogens. 2020-11-27T09:54:02Z 2020-11-27T09:54:02Z 2016 article publishedVersion Vilanova, L., Teixidó, N., Torres, R., Usall, J., Viñas, I., & Sánchez-Torres, P. (2016). Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection. International journal of food microbiology, 235, 93-102. 0168-1605 http://hdl.handle.net/20.500.11939/6838 10.1016/j.ijfoodmicro.2016.07.027 https://www.sciencedirect.com/science/article/abs/pii/S0168160516303816 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Conidiation Fruit-pathogen interaction Q02 Food processing and preservation J10 Handling, transport, storage and protection of agricultural products Virulence Transcription factors Penicillium digitatum Citrus Vilanova, Laura Teixidó, Neus Torres, Rosario Usall, Josep Vinas, Inmaculada Sánchez-Torres, Paloma Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title | Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title_full | Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title_fullStr | Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title_full_unstemmed | Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title_short | Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection |
| title_sort | relevance of the transcription factor pdste12 in penicillium digitatum conidiation and virulence during citrus fruit infection |
| topic | Conidiation Fruit-pathogen interaction Q02 Food processing and preservation J10 Handling, transport, storage and protection of agricultural products Virulence Transcription factors Penicillium digitatum Citrus |
| url | http://hdl.handle.net/20.500.11939/6838 https://www.sciencedirect.com/science/article/abs/pii/S0168160516303816 |
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