Molecular Characterization of Olive Cultivars Using RAPD Markers
Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
ASHS
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6756 https://journals.ashs.org/jashs/view/journals/jashs/126/1/article-p7.xml |
| _version_ | 1855032484259954688 |
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| author | Sanz-Cortés, F. Badenes, María L. Paz, Sergio Iniguez, Arturo Llácer, Gerardo |
| author_browse | Badenes, María L. Iniguez, Arturo Llácer, Gerardo Paz, Sergio Sanz-Cortés, F. |
| author_facet | Sanz-Cortés, F. Badenes, María L. Paz, Sergio Iniguez, Arturo Llácer, Gerardo |
| author_sort | Sanz-Cortés, F. |
| collection | ReDivia |
| description | Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used
to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis of their similarity values revealed two main groups divided according to geographic origin within Valencia. A third group,
which included two Spanish cultivars from regions outside of Valencia, was clustered separately from the Valencian cultivars. RAPD technology proved useful in discriminating closely related cultivars. There was no apparent clustering of cultivars by fruit size or other morphological traits. |
| format | article |
| id | ReDivia6756 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | ASHS |
| publisherStr | ASHS |
| record_format | dspace |
| spelling | ReDivia67562025-04-25T14:47:48Z Molecular Characterization of Olive Cultivars Using RAPD Markers Sanz-Cortés, F. Badenes, María L. Paz, Sergio Iniguez, Arturo Llácer, Gerardo Genetic typing Cultivar identification F30 Plant genetics and breeding F70 Plant taxonomy and geography Olea europaea Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis of their similarity values revealed two main groups divided according to geographic origin within Valencia. A third group, which included two Spanish cultivars from regions outside of Valencia, was clustered separately from the Valencian cultivars. RAPD technology proved useful in discriminating closely related cultivars. There was no apparent clustering of cultivars by fruit size or other morphological traits. 2020-11-16T12:31:37Z 2020-11-16T12:31:37Z 2001 article publishedVersion Sanz-Cortés, F., Badenes, M. L., Paz, S., Iniguez, A. & Llacer, G. (2001). Molecular characterization of olive cultivars using RAPD markers. Journal of the American Society for Horticultural Science, 126(1), 7-12. 0003-1062 2327-9788 (Online ISSN) http://hdl.handle.net/20.500.11939/6756 10.21273/JASHS.126.1.07 https://journals.ashs.org/jashs/view/journals/jashs/126/1/article-p7.xml en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess ASHS electronico |
| spellingShingle | Genetic typing Cultivar identification F30 Plant genetics and breeding F70 Plant taxonomy and geography Olea europaea Sanz-Cortés, F. Badenes, María L. Paz, Sergio Iniguez, Arturo Llácer, Gerardo Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title | Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title_full | Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title_fullStr | Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title_full_unstemmed | Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title_short | Molecular Characterization of Olive Cultivars Using RAPD Markers |
| title_sort | molecular characterization of olive cultivars using rapd markers |
| topic | Genetic typing Cultivar identification F30 Plant genetics and breeding F70 Plant taxonomy and geography Olea europaea |
| url | http://hdl.handle.net/20.500.11939/6756 https://journals.ashs.org/jashs/view/journals/jashs/126/1/article-p7.xml |
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