Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine
Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be recovered from 2x 9 2x sexual hybridizations as a consequence of the formation of unreduced gametes (2n), or from 4x 9 2x interploid hybridizations in which tetraploid parents used are most...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6755 https://link.springer.com/article/10.1007/s00299-016-1972-4 |
| _version_ | 1855492144922361856 |
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| author | Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_browse | Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Triploid breeding is developed worldwide to
create new seedless cultivars. Citrus triploid hybrids can be
recovered from 2x 9 2x sexual hybridizations as a consequence
of the formation of unreduced gametes (2n), or
from 4x 9 2x interploid hybridizations in which tetraploid
parents used are most often doubled-diploid (DD). Here we
have analyzed the inheritance in doubled-diploid clementine
and compared the genetic structures of gametes of DD
clementine with SDR unreduced gametes of diploid
clementine. Parental heterozygosity restitution (PHR) with
DD parents depends on the rate of preferential chromosome
pairing and thus the proportion of disomic versus
tetrasomic segregations. Doubled-diploid clementine largely
exhibited tetrasomic segregation. However, three
linkage groups had intermediate segregation and one had a
tendency for disomy. Significant doubled reduction rates
(DR) rates were observed in six of the nine LGs. Differences
of PHR between 2n SDR and 2x DD gametes were
highest in the centromeric region and progressively
decreased toward the distal regions where they were not
significant. Over all markers, PHR was lower (two-thirds)
in SDR 2n gametes than in DD-derived diploid gametes.
The two strategies appear complementary in terms of
genotypic variability. Interploid 4x 9 2x hybridization is
potentially more efficient for developing new cultivars that
are phenotypically closer to the diploid parent of the DD
than sexual hybridization through SDR 2n gametes. Conversely,
2x 9 2x triploidisation has the potential to produce
novel products with characteristics for market
segmentation strategies. |
| format | Artículo |
| id | ReDivia6755 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia67552025-04-25T14:47:48Z Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick Triploid SSR and SNP markers Second division restitution Tetrasomic Disomic F30 Plant genetics and breeding Citrus Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be recovered from 2x 9 2x sexual hybridizations as a consequence of the formation of unreduced gametes (2n), or from 4x 9 2x interploid hybridizations in which tetraploid parents used are most often doubled-diploid (DD). Here we have analyzed the inheritance in doubled-diploid clementine and compared the genetic structures of gametes of DD clementine with SDR unreduced gametes of diploid clementine. Parental heterozygosity restitution (PHR) with DD parents depends on the rate of preferential chromosome pairing and thus the proportion of disomic versus tetrasomic segregations. Doubled-diploid clementine largely exhibited tetrasomic segregation. However, three linkage groups had intermediate segregation and one had a tendency for disomy. Significant doubled reduction rates (DR) rates were observed in six of the nine LGs. Differences of PHR between 2n SDR and 2x DD gametes were highest in the centromeric region and progressively decreased toward the distal regions where they were not significant. Over all markers, PHR was lower (two-thirds) in SDR 2n gametes than in DD-derived diploid gametes. The two strategies appear complementary in terms of genotypic variability. Interploid 4x 9 2x hybridization is potentially more efficient for developing new cultivars that are phenotypically closer to the diploid parent of the DD than sexual hybridization through SDR 2n gametes. Conversely, 2x 9 2x triploidisation has the potential to produce novel products with characteristics for market segmentation strategies. 2020-11-16T12:25:13Z 2020-11-16T12:25:13Z 2016 article publishedVersion Aleza, P., Cuenca, J., Juárez, J., Navarro, L., & Ollitrault, P. (2016). Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine. Plant Cell Reports, 35(8), 1573-1586. 0721-7714 1432-203X (eISSN) http://hdl.handle.net/20.500.11939/6755 10.1007/s00299-016-1972-4 https://link.springer.com/article/10.1007/s00299-016-1972-4 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Springer electronico |
| spellingShingle | Triploid SSR and SNP markers Second division restitution Tetrasomic Disomic F30 Plant genetics and breeding Citrus Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title | Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title_full | Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title_fullStr | Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title_full_unstemmed | Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title_short | Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine |
| title_sort | inheritance in doubled diploid clementine and comparative study with sdr unreduced gametes of diploid clementine |
| topic | Triploid SSR and SNP markers Second division restitution Tetrasomic Disomic F30 Plant genetics and breeding Citrus |
| url | http://hdl.handle.net/20.500.11939/6755 https://link.springer.com/article/10.1007/s00299-016-1972-4 |
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