Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids
Recent research suggests that systemic signalling and communication between roots and leaves plays an important role in plant defence against herbivores. In the present study, we show that the oviposition of the two-spotted spider mite Tetranychus urticae in the systemic leaves of citrus rootstock C...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Oxford University Press
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6742 https://academic.oup.com/jxb/article/67/19/5711/2236365 |
| _version_ | 1855492142351253504 |
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| author | Agut, Blas Gamir, Jordi Jaques, Josep A. Flors, Victor |
| author_browse | Agut, Blas Flors, Victor Gamir, Jordi Jaques, Josep A. |
| author_facet | Agut, Blas Gamir, Jordi Jaques, Josep A. Flors, Victor |
| author_sort | Agut, Blas |
| collection | ReDivia |
| description | Recent research suggests that systemic signalling and communication between roots and leaves plays an important role in plant defence against herbivores. In the present study, we show that the oviposition of the two-spotted spider mite Tetranychus urticae in the systemic leaves of citrus rootstock Citrus aurantium (sour orange) was reduced by 50% when a lower leaf was previously infested with conspecifics. Metabolomic and gene expression analysis of the root efflux revealed a strong accumulation of glutamic acid (Glu) that triggered the expression of the citrus putative glutamate receptor ( GRL ) in the shoots. Additionally, uninfested sour orange systemic leaves showed increased expression of glutamate receptors and higher amounts of jasmonic acid (JA) and 12-oxo-phytodienoic acid in plants that were previously infested. Glu perception in the shoots induced the JA pathway, which primed LOX-2 gene expression when citrus plants were exposed to a second infestation. The spider mite-susceptible citrus rootstock Cleopatra mandarin ( C. unshiu ) also expressed systemic resistance, although the resistance was less effective than the resistance in sour orange. Surprisingly, the mobile signal in Cleopatra mandarin was not Glu, which suggests a strong genotype-dependency for systemic signalling in citrus. When the cultivar Clemenules ( C. clementina ) was grafted onto sour orange, there was a reduction in symptomatic leaves and T. urticae populations compared to the same cultivar grafted onto Cleopatra mandarin. Thus, systemic resistance is transmitted from the roots to the shoots in citrus and is dependent on rootstock resistance. |
| format | Artículo |
| id | ReDivia6742 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia67422025-04-25T14:47:46Z Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids Agut, Blas Gamir, Jordi Jaques, Josep A. Flors, Victor Glutamate-receptor like Systemic resistance H10 Pests of plants Citrus Glutamic acid Grafting Tetranychus urticae Recent research suggests that systemic signalling and communication between roots and leaves plays an important role in plant defence against herbivores. In the present study, we show that the oviposition of the two-spotted spider mite Tetranychus urticae in the systemic leaves of citrus rootstock Citrus aurantium (sour orange) was reduced by 50% when a lower leaf was previously infested with conspecifics. Metabolomic and gene expression analysis of the root efflux revealed a strong accumulation of glutamic acid (Glu) that triggered the expression of the citrus putative glutamate receptor ( GRL ) in the shoots. Additionally, uninfested sour orange systemic leaves showed increased expression of glutamate receptors and higher amounts of jasmonic acid (JA) and 12-oxo-phytodienoic acid in plants that were previously infested. Glu perception in the shoots induced the JA pathway, which primed LOX-2 gene expression when citrus plants were exposed to a second infestation. The spider mite-susceptible citrus rootstock Cleopatra mandarin ( C. unshiu ) also expressed systemic resistance, although the resistance was less effective than the resistance in sour orange. Surprisingly, the mobile signal in Cleopatra mandarin was not Glu, which suggests a strong genotype-dependency for systemic signalling in citrus. When the cultivar Clemenules ( C. clementina ) was grafted onto sour orange, there was a reduction in symptomatic leaves and T. urticae populations compared to the same cultivar grafted onto Cleopatra mandarin. Thus, systemic resistance is transmitted from the roots to the shoots in citrus and is dependent on rootstock resistance. 2020-11-11T09:16:59Z 2020-11-11T09:16:59Z 2016 article publishedVersion Agut, B., Gamir, J., Jaques, J. A., & Flors, V. (2016). Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids. Journal of Experimental Botany, 67(19), 5711-5723. 0022-0957 (Print ISSN) 1460-2431 (Online ISSN) http://hdl.handle.net/20.500.11939/6742 10.1093/jxb/erw335 https://academic.oup.com/jxb/article/67/19/5711/2236365 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Oxford University Press electronico |
| spellingShingle | Glutamate-receptor like Systemic resistance H10 Pests of plants Citrus Glutamic acid Grafting Tetranychus urticae Agut, Blas Gamir, Jordi Jaques, Josep A. Flors, Victor Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title | Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title_full | Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title_fullStr | Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title_full_unstemmed | Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title_short | Systemic resistance in citrus to Tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| title_sort | systemic resistance in citrus to tetranychus urticae induced by conspecifics is transmitted by grafting and mediated by mobile amino acids |
| topic | Glutamate-receptor like Systemic resistance H10 Pests of plants Citrus Glutamic acid Grafting Tetranychus urticae |
| url | http://hdl.handle.net/20.500.11939/6742 https://academic.oup.com/jxb/article/67/19/5711/2236365 |
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