Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production

Successful haploid induction in loquat (Eriobotrya japonica (Thunb.) Lindl.) through in situ-induced parthenogenesis with gamma-ray irradiated pollen has been achieved. Female flowers of cultivar ‘Algerie’ were pollinated using pollen of cultivars ‘Changhong-3’, ‘Cox’ and ‘Saval Brasil’ irradiated w...

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Autores principales: Blasco, Manuel, Badenes, María L., Naval, María M.
Formato: Artículo
Lenguaje:Inglés
Publicado: Japanese Society of Breeding 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/6737
https://www.jstage.jst.go.jp/article/jsbbs/66/4/66_16021/_article
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author Blasco, Manuel
Badenes, María L.
Naval, María M.
author_browse Badenes, María L.
Blasco, Manuel
Naval, María M.
author_facet Blasco, Manuel
Badenes, María L.
Naval, María M.
author_sort Blasco, Manuel
collection ReDivia
description Successful haploid induction in loquat (Eriobotrya japonica (Thunb.) Lindl.) through in situ-induced parthenogenesis with gamma-ray irradiated pollen has been achieved. Female flowers of cultivar ‘Algerie’ were pollinated using pollen of cultivars ‘Changhong-3’, ‘Cox’ and ‘Saval Brasil’ irradiated with two doses of gamma rays, 150 and 300 Gy. The fruits were harvested 90, 105 and 120 days after pollination (dap). Four haploid plants were obtained from ‘Algerie’ pollinated with 300-Gy-treated pollen of ‘Saval Brasil’ from fruits harvested 105 dap. Haploidy was confirmed by flow cytometry and chromosome count. The haploids showed a very weak development compared to the diploid plants. This result suggests that irradiated pollen can be used to obtain parthenogenetic haploids.
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language Inglés
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publisherStr Japanese Society of Breeding
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spelling ReDivia67372025-04-25T14:47:45Z Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production Blasco, Manuel Badenes, María L. Naval, María M. Gamma-ray irradiation Irradiation dose Ploidy determination F30 Plant genetics and breeding Eriobotrya japonica Haploidy Successful haploid induction in loquat (Eriobotrya japonica (Thunb.) Lindl.) through in situ-induced parthenogenesis with gamma-ray irradiated pollen has been achieved. Female flowers of cultivar ‘Algerie’ were pollinated using pollen of cultivars ‘Changhong-3’, ‘Cox’ and ‘Saval Brasil’ irradiated with two doses of gamma rays, 150 and 300 Gy. The fruits were harvested 90, 105 and 120 days after pollination (dap). Four haploid plants were obtained from ‘Algerie’ pollinated with 300-Gy-treated pollen of ‘Saval Brasil’ from fruits harvested 105 dap. Haploidy was confirmed by flow cytometry and chromosome count. The haploids showed a very weak development compared to the diploid plants. This result suggests that irradiated pollen can be used to obtain parthenogenetic haploids. 2020-11-10T08:53:04Z 2020-11-10T08:53:04Z 2016 article publishedVersion Blasco, M., Badenes, M. L. & Naval, M. M. (2016). Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production. Breeding science, 66(4), 606-612. 1344-7610 (PRINT ISSN) 1347-3735 (ONLINE ISSN) http://hdl.handle.net/20.500.11939/6737 10.1270%2Fjsbbs.16021 https://www.jstage.jst.go.jp/article/jsbbs/66/4/66_16021/_article en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Japanese Society of Breeding electronico
spellingShingle Gamma-ray irradiation
Irradiation dose
Ploidy determination
F30 Plant genetics and breeding
Eriobotrya japonica
Haploidy
Blasco, Manuel
Badenes, María L.
Naval, María M.
Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title_full Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title_fullStr Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title_full_unstemmed Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title_short Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production
title_sort induced parthenogenesis by gamma irradiated pollen in loquat for haploid production
topic Gamma-ray irradiation
Irradiation dose
Ploidy determination
F30 Plant genetics and breeding
Eriobotrya japonica
Haploidy
url http://hdl.handle.net/20.500.11939/6737
https://www.jstage.jst.go.jp/article/jsbbs/66/4/66_16021/_article
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