Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection
Natural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. Analysis of the corresponding populations showed that they were exclusively compose...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6714 https://www.nature.com/articles/srep42825 |
| _version_ | 1855032476395634688 |
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| author | Serra, Pedro Bertolini, Edson Martínez, M. Carmen Cambra, Mariano Flores, Ricardo |
| author_browse | Bertolini, Edson Cambra, Mariano Flores, Ricardo Martínez, M. Carmen Serra, Pedro |
| author_facet | Serra, Pedro Bertolini, Edson Martínez, M. Carmen Cambra, Mariano Flores, Ricardo |
| author_sort | Serra, Pedro |
| collection | ReDivia |
| description | Natural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. Analysis of the corresponding populations showed that they were exclusively composed of variants (of class II) with a structural domain different from that of the reference and many other variants (of class I) targeted by the TaqMan rtRT-PCR probe. Bioassays in peach revealed that a representative PLMVd variant of class II replicated without symptoms, generated a progeny with low nucleotide diversity, and, intriguingly, outcompeted a representative symptomatic variant of class I when co-inoculated in equimolecular amounts. A number of informative positions associated with the higher fitness of variants of class II have been identified, and novel sets of primers and probes for universal or specific TaqMan rtRT-PCR detection of PLMVd variants have been designed and tested. |
| format | article |
| id | ReDivia6714 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia67142025-04-25T14:47:42Z Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection Serra, Pedro Bertolini, Edson Martínez, M. Carmen Cambra, Mariano Flores, Ricardo Virus-host interactions H20 Plant diseases Virology Natural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. Analysis of the corresponding populations showed that they were exclusively composed of variants (of class II) with a structural domain different from that of the reference and many other variants (of class I) targeted by the TaqMan rtRT-PCR probe. Bioassays in peach revealed that a representative PLMVd variant of class II replicated without symptoms, generated a progeny with low nucleotide diversity, and, intriguingly, outcompeted a representative symptomatic variant of class I when co-inoculated in equimolecular amounts. A number of informative positions associated with the higher fitness of variants of class II have been identified, and novel sets of primers and probes for universal or specific TaqMan rtRT-PCR detection of PLMVd variants have been designed and tested. 2020-10-28T12:41:29Z 2020-10-28T12:41:29Z 2017 article publishedVersion Serra, P., Bertolini, E., Martínez, M. C., Cambra, M., & Flores, R. (2017). Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection. Scientific reports, 7, 42825. 2045-2322 (online) http://hdl.handle.net/20.500.11939/6714 10.1038/srep42825 https://www.nature.com/articles/srep42825 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer Nature electronico |
| spellingShingle | Virus-host interactions H20 Plant diseases Virology Serra, Pedro Bertolini, Edson Martínez, M. Carmen Cambra, Mariano Flores, Ricardo Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title_full | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title_fullStr | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title_full_unstemmed | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title_short | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection |
| title_sort | interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape implications for detection |
| topic | Virus-host interactions H20 Plant diseases Virology |
| url | http://hdl.handle.net/20.500.11939/6714 https://www.nature.com/articles/srep42825 |
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