Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones
Tomato black ring virus (TBRV, genus Nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engin...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6702 https://www.sciencedirect.com/science/article/abs/pii/S0168170216306864 |
| _version_ | 1855032474228228096 |
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| author | Zarzyńska-Nowak, Aleksandra Ferriol, Inmaculada Falk, Bryce W. Borodynko-Filas, Natasza Hasiów-Jaroszewska, Beata |
| author_browse | Borodynko-Filas, Natasza Falk, Bryce W. Ferriol, Inmaculada Hasiów-Jaroszewska, Beata Zarzyńska-Nowak, Aleksandra |
| author_facet | Zarzyńska-Nowak, Aleksandra Ferriol, Inmaculada Falk, Bryce W. Borodynko-Filas, Natasza Hasiów-Jaroszewska, Beata |
| author_sort | Zarzyńska-Nowak, Aleksandra |
| collection | ReDivia |
| description | Tomato black ring virus (TBRV, genus Nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS). The symptoms induced on plants agroinoculated with both constructs were indistinguishable from those caused by the wild-type virus. The infectivity of obtained clones was verified by reinoculation to Nicotiana tabacum cv. Xanthi, Chenopodium quinoa and Cucumis sativus. The presence of viral particles and RNA was confirmed by electron microscopy and reverse transcription polymerase chain reaction, respectively. Constructed full-length infectious cDNA clones will serve as an excellent tool to study virus-host-vector interactions. |
| format | article |
| id | ReDivia6702 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia67022025-04-25T14:47:41Z Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones Zarzyńska-Nowak, Aleksandra Ferriol, Inmaculada Falk, Bryce W. Borodynko-Filas, Natasza Hasiów-Jaroszewska, Beata Tomato black ring virus TBRV cDNA infectious clones Agroinfiltration F30 Plant genetics and breeding H20 Plant diseases Tomato black ring virus (TBRV, genus Nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS). The symptoms induced on plants agroinoculated with both constructs were indistinguishable from those caused by the wild-type virus. The infectivity of obtained clones was verified by reinoculation to Nicotiana tabacum cv. Xanthi, Chenopodium quinoa and Cucumis sativus. The presence of viral particles and RNA was confirmed by electron microscopy and reverse transcription polymerase chain reaction, respectively. Constructed full-length infectious cDNA clones will serve as an excellent tool to study virus-host-vector interactions. 2020-10-28T10:57:53Z 2020-10-28T10:57:53Z 2017 article publishedVersion Zarzyńska-Nowak, A., Ferriol, I., Falk, B. W., Borodynko-Filas, N., & Hasiów-Jaroszewska, B. (2017). Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones. Virus research, 230, 59-62. 0168-1702 http://hdl.handle.net/20.500.11939/6702 10.1016/j.virusres.2017.01.014 https://www.sciencedirect.com/science/article/abs/pii/S0168170216306864 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Tomato black ring virus TBRV cDNA infectious clones Agroinfiltration F30 Plant genetics and breeding H20 Plant diseases Zarzyńska-Nowak, Aleksandra Ferriol, Inmaculada Falk, Bryce W. Borodynko-Filas, Natasza Hasiów-Jaroszewska, Beata Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title | Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title_full | Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title_fullStr | Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title_full_unstemmed | Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title_short | Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones |
| title_sort | construction of agrobacterium tumefaciens mediated tomato black ring virus infectious cdna clones |
| topic | Tomato black ring virus TBRV cDNA infectious clones Agroinfiltration F30 Plant genetics and breeding H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6702 https://www.sciencedirect.com/science/article/abs/pii/S0168170216306864 |
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