Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm
Several factors unrelated to the semen samples could be influencing in the sperm motility analysis. The aim of the present research was to study the effect of four chambers with different characteristics, namely; slide-coverslip, Spermtrack, ISAS D4C10, and ISAS D4C20 on the sperm motility. The fill...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6692 https://www.sciencedirect.com/science/article/abs/pii/S0378432016302925 |
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| author | Del-Gallego, Raquel Sadeghi, Sara Blasco, Emilio Soler, Carlos Yániz, Jesús L. Silvestre, Miguel A. |
| author_browse | Blasco, Emilio Del-Gallego, Raquel Sadeghi, Sara Silvestre, Miguel A. Soler, Carlos Yániz, Jesús L. |
| author_facet | Del-Gallego, Raquel Sadeghi, Sara Blasco, Emilio Soler, Carlos Yániz, Jesús L. Silvestre, Miguel A. |
| author_sort | Del-Gallego, Raquel |
| collection | ReDivia |
| description | Several factors unrelated to the semen samples could be influencing in the sperm motility analysis. The aim of the present research was to study the effect of four chambers with different characteristics, namely; slide-coverslip, Spermtrack, ISAS D4C10, and ISAS D4C20 on the sperm motility. The filling procedure (drop or capillarity) and analysis time (0, 120 and 240 s), depth of chamber (10 or 20 μm) and field on motility variables were analysed by use of the CASA-mot system in goat sperm. Use of the drop-filling chambers resulted in greater values than capillarity-filling chambers for all sperm motility and kinetic variables, except for LIN (64.5% compared with 56.3% of motility for drop- and capillarity-filling chambers respectively, P < 0.05). There were no significant differences in total sperm motility between different chamber depths, however, use of the 20 μm-chambers resulted in greater sperm progressive motility rate, VSL and LIN, and less VCL and VAP than chambers with a lesser depth. There was less sperm motility and lesser values for kinetic variables as time that elapsed increased between sample loading and sperm evaluation. For sperm motility, use of droplet-loaded chambers resulted in similar values of MOT in all microscopic fields, but sperm motility assessed in capillarity-loaded chambers was less in the central fields than in the outermost microscopic fields. For goats, it is recommended that sperm motility be analysed using the CASA-mot system with a drop-loaded chamber within 2 min after filling the chamber. |
| format | Artículo |
| id | ReDivia6692 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia66922025-04-25T14:47:40Z Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm Del-Gallego, Raquel Sadeghi, Sara Blasco, Emilio Soler, Carlos Yániz, Jesús L. Silvestre, Miguel A. Sperm motility Chamber ISAS® CASA-mot Loading technique Goats Several factors unrelated to the semen samples could be influencing in the sperm motility analysis. The aim of the present research was to study the effect of four chambers with different characteristics, namely; slide-coverslip, Spermtrack, ISAS D4C10, and ISAS D4C20 on the sperm motility. The filling procedure (drop or capillarity) and analysis time (0, 120 and 240 s), depth of chamber (10 or 20 μm) and field on motility variables were analysed by use of the CASA-mot system in goat sperm. Use of the drop-filling chambers resulted in greater values than capillarity-filling chambers for all sperm motility and kinetic variables, except for LIN (64.5% compared with 56.3% of motility for drop- and capillarity-filling chambers respectively, P < 0.05). There were no significant differences in total sperm motility between different chamber depths, however, use of the 20 μm-chambers resulted in greater sperm progressive motility rate, VSL and LIN, and less VCL and VAP than chambers with a lesser depth. There was less sperm motility and lesser values for kinetic variables as time that elapsed increased between sample loading and sperm evaluation. For sperm motility, use of droplet-loaded chambers resulted in similar values of MOT in all microscopic fields, but sperm motility assessed in capillarity-loaded chambers was less in the central fields than in the outermost microscopic fields. For goats, it is recommended that sperm motility be analysed using the CASA-mot system with a drop-loaded chamber within 2 min after filling the chamber. 2020-10-27T08:07:20Z 2020-10-27T08:07:20Z 2017 article publishedVersion Del Gallego, R., Sadeghi, S., Blasco, E., Soler, C., Yániz, J. L. & Silvestre, M. A. (2017). Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm. Animal reproduction science, 177, 97-104. 0378-4320 http://hdl.handle.net/20.500.11939/6692 10.1016/j.anireprosci.2016.12.010 https://www.sciencedirect.com/science/article/abs/pii/S0378432016302925 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Sperm motility Chamber ISAS® CASA-mot Loading technique Goats Del-Gallego, Raquel Sadeghi, Sara Blasco, Emilio Soler, Carlos Yániz, Jesús L. Silvestre, Miguel A. Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title | Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title_full | Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title_fullStr | Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title_full_unstemmed | Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title_short | Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm |
| title_sort | effect of chamber characteristics loading and analysis time on motility and kinetic variables analysed with the casa mot system in goat sperm |
| topic | Sperm motility Chamber ISAS® CASA-mot Loading technique Goats |
| url | http://hdl.handle.net/20.500.11939/6692 https://www.sciencedirect.com/science/article/abs/pii/S0378432016302925 |
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