PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection.
The Slt2 mitogen-activated protein (MAP) kinase homologue of Penicillium digitatum, the most relevant pathogen-producing citrus green mould decay during postharvest, was identified and explored. The P. digitatum Slt2-MAPK coding gene (PdSlt2) was functionally characterized by homologous gene elimina...
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| Format: | article |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6691 https://www.sciencedirect.com/science/article/abs/pii/S1878614617301277 |
| _version_ | 1855032472166727680 |
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| author | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_browse | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_facet | De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma |
| author_sort | De-Ramón-Carbonell, Marta |
| collection | ReDivia |
| description | The Slt2 mitogen-activated protein (MAP) kinase homologue of Penicillium digitatum, the most relevant pathogen-producing citrus green mould decay during postharvest, was identified and explored. The P. digitatum Slt2-MAPK coding gene (PdSlt2) was functionally characterized by homologous gene elimination and transcriptomic evaluation. The absence of PdSlt2 gene resulted in significantly reduced virulence during citrus infection. The ΔPdSlt2 mutants were also defective in asexual reproduction, showing impairment of sporulation during citrus infection. Gene expression analysis revealed that PdSlt2 was highly induced during citrus fruit infection at early stages (1 dpi). Moreover, PdSlt2 deletion altered gene expression profiles. The relative gene expression (RGE) of fungicide resistance- and fungal virulence-related genes showed that PdSlt2 acts as negative regulator of several transporter encoding genes (ABC and MFS transporters) and a positive regulator of two sterol demethylases. This study indicates that PdSlt2 MAPK is functionally preserved in P. digitatum and highlights the relevant role of the PdSlt2 MAP kinase-mediated signalling pathway in regulating diverse genes crucial for infection and asexual reproduction. |
| format | article |
| id | ReDivia6691 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia66912025-04-25T14:47:40Z PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma Conidiogenesis Fungal-fruit interaction MAP kinase H20 Plant diseases Gene expression Penicillium digitatum Virulence The Slt2 mitogen-activated protein (MAP) kinase homologue of Penicillium digitatum, the most relevant pathogen-producing citrus green mould decay during postharvest, was identified and explored. The P. digitatum Slt2-MAPK coding gene (PdSlt2) was functionally characterized by homologous gene elimination and transcriptomic evaluation. The absence of PdSlt2 gene resulted in significantly reduced virulence during citrus infection. The ΔPdSlt2 mutants were also defective in asexual reproduction, showing impairment of sporulation during citrus infection. Gene expression analysis revealed that PdSlt2 was highly induced during citrus fruit infection at early stages (1 dpi). Moreover, PdSlt2 deletion altered gene expression profiles. The relative gene expression (RGE) of fungicide resistance- and fungal virulence-related genes showed that PdSlt2 acts as negative regulator of several transporter encoding genes (ABC and MFS transporters) and a positive regulator of two sterol demethylases. This study indicates that PdSlt2 MAPK is functionally preserved in P. digitatum and highlights the relevant role of the PdSlt2 MAP kinase-mediated signalling pathway in regulating diverse genes crucial for infection and asexual reproduction. 2020-10-27T08:05:41Z 2020-10-27T08:05:41Z 2017 article publishedVersion de Ramón-Carbonell, M., & Sánchez-Torres, P. (2017). PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. Fungal biology, 121(12), 1063-1074. 1878-6146 http://hdl.handle.net/20.500.11939/6691 10.1016/j.funbio.2017.09.004 https://www.sciencedirect.com/science/article/abs/pii/S1878614617301277 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Conidiogenesis Fungal-fruit interaction MAP kinase H20 Plant diseases Gene expression Penicillium digitatum Virulence De-Ramón-Carbonell, Marta Sánchez-Torres, Paloma PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title | PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title_full | PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title_fullStr | PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title_full_unstemmed | PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title_short | PdSlt2 Penicillium digitatum mitogen-activated-protein kinase controls sporulation and virulence during citrus fruit infection. |
| title_sort | pdslt2 penicillium digitatum mitogen activated protein kinase controls sporulation and virulence during citrus fruit infection |
| topic | Conidiogenesis Fungal-fruit interaction MAP kinase H20 Plant diseases Gene expression Penicillium digitatum Virulence |
| url | http://hdl.handle.net/20.500.11939/6691 https://www.sciencedirect.com/science/article/abs/pii/S1878614617301277 |
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