Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence
Citrus is an extremely important genus in terms of world fruit production. Despite its economic importance and the small genome sizes of its species (2n = 18, 1C = 430 ± 68 Mbp), entire genomic assemblies have only recently become available for some of its representatives. Together with the previous...
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| Format: | Artículo |
| Language: | Inglés |
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Canadian Science Publishing (CSP)
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6689 https://cdnsciencepub.com/doi/abs/10.1139/gen-2020-0046 |
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| author | Mendes, Sandra Régis, Thallita Terol, Javier Dos Santos Soares filho, Walter Talón, Manuel Pedrosa-Harand, Andrea |
| author_browse | Dos Santos Soares filho, Walter Mendes, Sandra Pedrosa-Harand, Andrea Régis, Thallita Talón, Manuel Terol, Javier |
| author_facet | Mendes, Sandra Régis, Thallita Terol, Javier Dos Santos Soares filho, Walter Talón, Manuel Pedrosa-Harand, Andrea |
| author_sort | Mendes, Sandra |
| collection | ReDivia |
| description | Citrus is an extremely important genus in terms of world fruit production. Despite its economic importance and the small genome sizes of its species (2n = 18, 1C = 430 ± 68 Mbp), entire genomic assemblies have only recently become available for some of its representatives. Together with the previous CMA/DAPI banding and fluorescence in situ hybridization (FISH) in the group, these data are important for understanding the complex relationships between its species and for assisting breeding programs. To anchor genomic data with the cytogenetic map of mandarin (Citrus reticulata), the parental species of several economically important hybrids such as sweet orange and clementine, 18 BAC (bacterial artificial chromosome) clones were used. Eleven clementine BACs were positioned by BAC-FISH, doubling the number of chromosome markers so far available for BAC-FISH in citrus. Additionally, six previously mapped BACs were end-sequenced, allowing, together with one BAC previously sequenced, their assignment to scaffolds and the subsequent integration of chromosomes and the genome assembly. This study therefore established correlations between mandarin scaffolds and chromosomes, allowing further structural genomic and comparative study with the sweet orange genome, as well as insights into the chromosomal evolution of the group |
| format | Artículo |
| id | ReDivia6689 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Canadian Science Publishing (CSP) |
| publisherStr | Canadian Science Publishing (CSP) |
| record_format | dspace |
| spelling | ReDivia66892025-04-25T14:47:40Z Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence Mendes, Sandra Régis, Thallita Terol, Javier Dos Santos Soares filho, Walter Talón, Manuel Pedrosa-Harand, Andrea F30 Plant genetics and breeding Genomes Citrus reticulata Cytogenetics Citrus is an extremely important genus in terms of world fruit production. Despite its economic importance and the small genome sizes of its species (2n = 18, 1C = 430 ± 68 Mbp), entire genomic assemblies have only recently become available for some of its representatives. Together with the previous CMA/DAPI banding and fluorescence in situ hybridization (FISH) in the group, these data are important for understanding the complex relationships between its species and for assisting breeding programs. To anchor genomic data with the cytogenetic map of mandarin (Citrus reticulata), the parental species of several economically important hybrids such as sweet orange and clementine, 18 BAC (bacterial artificial chromosome) clones were used. Eleven clementine BACs were positioned by BAC-FISH, doubling the number of chromosome markers so far available for BAC-FISH in citrus. Additionally, six previously mapped BACs were end-sequenced, allowing, together with one BAC previously sequenced, their assignment to scaffolds and the subsequent integration of chromosomes and the genome assembly. This study therefore established correlations between mandarin scaffolds and chromosomes, allowing further structural genomic and comparative study with the sweet orange genome, as well as insights into the chromosomal evolution of the group 2020-10-23T12:29:20Z 2020-10-23T12:29:20Z 2020 article publishedVersion Mendes, S., Régis, T., Terol, J., Dos-Santos-Soares Filho, W., Talon, M., & Pedrosa-Harand, A. (2020). Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence. Genome, 63(9), 437-444. 0831-2796 (Print-ISSN) 1480-3321 (e-ISSN) http://hdl.handle.net/20.500.11939/6689 10.1139/gen-2020-0046 https://cdnsciencepub.com/doi/abs/10.1139/gen-2020-0046 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Canadian Science Publishing (CSP) electronico |
| spellingShingle | F30 Plant genetics and breeding Genomes Citrus reticulata Cytogenetics Mendes, Sandra Régis, Thallita Terol, Javier Dos Santos Soares filho, Walter Talón, Manuel Pedrosa-Harand, Andrea Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title | Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title_full | Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title_fullStr | Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title_full_unstemmed | Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title_short | Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence |
| title_sort | integration of mandarin citrus reticulata cytogenetic map with its genome sequence |
| topic | F30 Plant genetics and breeding Genomes Citrus reticulata Cytogenetics |
| url | http://hdl.handle.net/20.500.11939/6689 https://cdnsciencepub.com/doi/abs/10.1139/gen-2020-0046 |
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