QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities

The most sustainable approach to overcome iron deficiency in fruit crops is breeding for rootstocks with a higher capability to acquire iron (Fe) from the soil. The objective of this study was quantitative trait loci (QTL) and candidate gene analyses of rootstock-mediated low-Fe tolerance in terms o...

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Autores principales: Asins, María J., Raga, Verónica, Roca, Dolors, Carbonell, Emilio A.
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/6684
https://rdcu.be/b8Rxc
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author Asins, María J.
Raga, Verónica
Roca, Dolors
Carbonell, Emilio A.
author_browse Asins, María J.
Carbonell, Emilio A.
Raga, Verónica
Roca, Dolors
author_facet Asins, María J.
Raga, Verónica
Roca, Dolors
Carbonell, Emilio A.
author_sort Asins, María J.
collection ReDivia
description The most sustainable approach to overcome iron deficiency in fruit crops is breeding for rootstocks with a higher capability to acquire iron (Fe) from the soil. The objective of this study was quantitative trait loci (QTL) and candidate gene analyses of rootstock-mediated low-Fe tolerance in terms of fruit yield and quality traits, including Fe fruit content, in a satsuma mandaringrafted rootstock population derived from a cross between Citrus reshni (Cleopatra mandarin) and Poncirus trifoliata, under sufficient and low-Fe fertilization (15.3 vs 5.2 μM Fe, respectively). Iron reduction to one-third significantly decreased satsuma leaf chlorophyll concentration, fruit iron concentration, and the fruit/leaf iron proportion. Thirty-four QTLs were detected for 46 heritable traits. Eighteen of them were also found significant when testing each parental genome separately. Seven QTLs contributed to the fruit concentrations of Cu, Fe, K, Na, and S. QTLs involved in rootstock mediated tolerance to Fe deficiency and fruit quality traits distributed into five genomic regions whose gene contents (assuming collinearity with the C. clementina genome) were investigated for overrepresented molecular functions and biological processes, and putative functional candidates. Among them, a metal-NA-transporter YSL3 (Ciclev 10019170m), four phytochelatin synthases, an iron-chelate-transporter ATPase, and four basic/helix-loop helix genes coding for likely relevant transcription factors in Fe homeostasis under Fe deficiency were found as follows: bHLH3 (Ciclev10019816m), bHLH137.1 (Ciclev10031873m), bHLH123 (Ciclev10008228m), and ILR3 (Ciclev10009354m). Genes within three QTL regions supported a genetic connection between rootstock-mediated tolerance to Fe deficiency and biotic stresses in citrus.
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spelling ReDivia66842025-04-25T14:47:39Z QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities Asins, María J. Raga, Verónica Roca, Dolors Carbonell, Emilio A. Rootstock breeding Iron deficiency Citrus reshni F30 Plant genetics and breeding F61 Plant physiology - Nutrition Poncirus trifoliata Citrus unshiu Disease resistance The most sustainable approach to overcome iron deficiency in fruit crops is breeding for rootstocks with a higher capability to acquire iron (Fe) from the soil. The objective of this study was quantitative trait loci (QTL) and candidate gene analyses of rootstock-mediated low-Fe tolerance in terms of fruit yield and quality traits, including Fe fruit content, in a satsuma mandaringrafted rootstock population derived from a cross between Citrus reshni (Cleopatra mandarin) and Poncirus trifoliata, under sufficient and low-Fe fertilization (15.3 vs 5.2 μM Fe, respectively). Iron reduction to one-third significantly decreased satsuma leaf chlorophyll concentration, fruit iron concentration, and the fruit/leaf iron proportion. Thirty-four QTLs were detected for 46 heritable traits. Eighteen of them were also found significant when testing each parental genome separately. Seven QTLs contributed to the fruit concentrations of Cu, Fe, K, Na, and S. QTLs involved in rootstock mediated tolerance to Fe deficiency and fruit quality traits distributed into five genomic regions whose gene contents (assuming collinearity with the C. clementina genome) were investigated for overrepresented molecular functions and biological processes, and putative functional candidates. Among them, a metal-NA-transporter YSL3 (Ciclev 10019170m), four phytochelatin synthases, an iron-chelate-transporter ATPase, and four basic/helix-loop helix genes coding for likely relevant transcription factors in Fe homeostasis under Fe deficiency were found as follows: bHLH3 (Ciclev10019816m), bHLH137.1 (Ciclev10031873m), bHLH123 (Ciclev10008228m), and ILR3 (Ciclev10009354m). Genes within three QTL regions supported a genetic connection between rootstock-mediated tolerance to Fe deficiency and biotic stresses in citrus. 2020-10-23T11:47:58Z 2020-10-23T11:47:58Z 2020 article acceptedVersion Asins, M.J., Raga, M.V., Roca, D. et al. QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities. Tree Genetics & Genomes 16, 79 (2020). https://doi.org/10.1007/s11295-020-01472-w 1614-2950 (e-ISSN) http://hdl.handle.net/20.500.11939/6684 10.1007/s11295-020-01472-w https://rdcu.be/b8Rxc en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ embargoedAccess Springer electronico
spellingShingle Rootstock breeding
Iron deficiency
Citrus reshni
F30 Plant genetics and breeding
F61 Plant physiology - Nutrition
Poncirus trifoliata
Citrus unshiu
Disease resistance
Asins, María J.
Raga, Verónica
Roca, Dolors
Carbonell, Emilio A.
QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title_full QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title_fullStr QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title_full_unstemmed QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title_short QTL and candidate gene analyses of rootstock-mediated mandarin fruit yield and quality traits under contrasting iron availabilities
title_sort qtl and candidate gene analyses of rootstock mediated mandarin fruit yield and quality traits under contrasting iron availabilities
topic Rootstock breeding
Iron deficiency
Citrus reshni
F30 Plant genetics and breeding
F61 Plant physiology - Nutrition
Poncirus trifoliata
Citrus unshiu
Disease resistance
url http://hdl.handle.net/20.500.11939/6684
https://rdcu.be/b8Rxc
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