Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions
Genes encoding HKT1-like Na+ transporters play a key role in the salinity tolerance mechanism in Arabidopsis and other plant species by retrieving Na+ from the xylem of different organs and tissues. In this study, we investigated the role of two HKT1;2 allelic variants in tomato salt tolerance in re...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6681 https://www.sciencedirect.com/science/article/abs/pii/S0981942820302369 |
| _version_ | 1855032470535143424 |
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| author | Romero-Aranda, María R. González-Fernández, Paloma Pérez-Tienda, Jacob R. López-Díaz, María R. Espinosa, Jesús Granum, Espen Traverso, Jose A. Pineda, Benito Garcia-Sogo, Begona Moreno, Vicente Asins, María J. Belver, Andres |
| author_browse | Asins, María J. Belver, Andres Espinosa, Jesús Garcia-Sogo, Begona González-Fernández, Paloma Granum, Espen López-Díaz, María R. Moreno, Vicente Pineda, Benito Pérez-Tienda, Jacob R. Romero-Aranda, María R. Traverso, Jose A. |
| author_facet | Romero-Aranda, María R. González-Fernández, Paloma Pérez-Tienda, Jacob R. López-Díaz, María R. Espinosa, Jesús Granum, Espen Traverso, Jose A. Pineda, Benito Garcia-Sogo, Begona Moreno, Vicente Asins, María J. Belver, Andres |
| author_sort | Romero-Aranda, María R. |
| collection | ReDivia |
| description | Genes encoding HKT1-like Na+ transporters play a key role in the salinity tolerance mechanism in Arabidopsis and other plant species by retrieving Na+ from the xylem of different organs and tissues. In this study, we investigated the role of two HKT1;2 allelic variants in tomato salt tolerance in relation to vegetative growth and fruit yield in plants subjected to salt treatment in a commercial greenhouse under real production conditions. We used two near-isogenic lines (NILs), homozygous for either the Solanum lycopersicum (NIL17) or S. cheesmaniae (NIL14) allele, at HKT1;2 loci and their respective RNAi-Sl/ScHKT1;2 lines. The results obtained show that both ScHKT1;2- and SlHKT1;2-silenced lines display hypersensitivity to salinity associated with an altered leaf Na+/K+ ratio, thus confirming that HKT1;2 plays an important role in Na+ homeostasis and salinity tolerance in tomato. Both silenced lines also showed Na+ over-accumulation and a slight, but significant, reduction in K+ content in the flower tissues of salt-treated plants and consequently a higher Na+/K+ ratio as compared to the respective unsilenced lines. This altered Na+/K+ ratio in flower tissues is associated with a sharp reduction in fruit yield, measured as total fresh weight and number of fruits, in both silenced lines under salinity conditions. Our findings demonstrate that Na+ transporter HKT1;2 protects the flower against Na+ toxicity and mitigates the reduction in tomato fruit yield under salinity conditions. |
| format | article |
| id | ReDivia6681 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia66812025-04-25T14:47:38Z Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions Romero-Aranda, María R. González-Fernández, Paloma Pérez-Tienda, Jacob R. López-Díaz, María R. Espinosa, Jesús Granum, Espen Traverso, Jose A. Pineda, Benito Garcia-Sogo, Begona Moreno, Vicente Asins, María J. Belver, Andres Na+ flower content Fruit yield HKT1 2gene K+ and Na+ homeostasis Solanum cheesmaniae F60 Plant physiology and biochemistry F40 Plant ecology Solanum lycopersicum Tomatoes Salinity Genes encoding HKT1-like Na+ transporters play a key role in the salinity tolerance mechanism in Arabidopsis and other plant species by retrieving Na+ from the xylem of different organs and tissues. In this study, we investigated the role of two HKT1;2 allelic variants in tomato salt tolerance in relation to vegetative growth and fruit yield in plants subjected to salt treatment in a commercial greenhouse under real production conditions. We used two near-isogenic lines (NILs), homozygous for either the Solanum lycopersicum (NIL17) or S. cheesmaniae (NIL14) allele, at HKT1;2 loci and their respective RNAi-Sl/ScHKT1;2 lines. The results obtained show that both ScHKT1;2- and SlHKT1;2-silenced lines display hypersensitivity to salinity associated with an altered leaf Na+/K+ ratio, thus confirming that HKT1;2 plays an important role in Na+ homeostasis and salinity tolerance in tomato. Both silenced lines also showed Na+ over-accumulation and a slight, but significant, reduction in K+ content in the flower tissues of salt-treated plants and consequently a higher Na+/K+ ratio as compared to the respective unsilenced lines. This altered Na+/K+ ratio in flower tissues is associated with a sharp reduction in fruit yield, measured as total fresh weight and number of fruits, in both silenced lines under salinity conditions. Our findings demonstrate that Na+ transporter HKT1;2 protects the flower against Na+ toxicity and mitigates the reduction in tomato fruit yield under salinity conditions. 2020-10-23T11:44:37Z 2020-10-23T11:44:37Z 2020 article acceptedVersion Romero-Aranda, M. R., Fernández, P. G., López-Tienda, J. R., López-Diaz, M. R., Espinosa, J., Granum, E., ... & Asins, M. J. (2020). Na+ transporter HKT1; 2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions. Plant Physiology and Biochemistry. 154, 341-352. 0981-9428 http://hdl.handle.net/20.500.11939/6681 10.1016/j.plaphy.2020.05.012 https://www.sciencedirect.com/science/article/abs/pii/S0981942820302369 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Elsevier electronico |
| spellingShingle | Na+ flower content Fruit yield HKT1 2gene K+ and Na+ homeostasis Solanum cheesmaniae F60 Plant physiology and biochemistry F40 Plant ecology Solanum lycopersicum Tomatoes Salinity Romero-Aranda, María R. González-Fernández, Paloma Pérez-Tienda, Jacob R. López-Díaz, María R. Espinosa, Jesús Granum, Espen Traverso, Jose A. Pineda, Benito Garcia-Sogo, Begona Moreno, Vicente Asins, María J. Belver, Andres Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title | Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title_full | Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title_fullStr | Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title_full_unstemmed | Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title_short | Na+ transporter HKT1;2 reduces flower Na+ content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| title_sort | na transporter hkt1 2 reduces flower na content and considerably mitigates the decline in tomato fruit yields under saline conditions |
| topic | Na+ flower content Fruit yield HKT1 2gene K+ and Na+ homeostasis Solanum cheesmaniae F60 Plant physiology and biochemistry F40 Plant ecology Solanum lycopersicum Tomatoes Salinity |
| url | http://hdl.handle.net/20.500.11939/6681 https://www.sciencedirect.com/science/article/abs/pii/S0981942820302369 |
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