Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain
Liberibacter is a bacterial group causing different diseases and disorders in plants. Among liberibacters, Candidatus Liberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes accordi...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6654 https://www.mdpi.com/2076-2607/8/9/1446 |
| _version_ | 1855492126086791168 |
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| author | Ruiz-Padilla, Ana Redondo, Cristina Asensio, Adrián Garita-Cambronero, Jerson Martínez, M. Carmen Pérez-Padilla, Verónica Marquínez, Raquel Collar, Jesús García-Méndez, Eva Alfaro-Fernández, Ana Asensio-S.-Manzanera, Carmen Palomo, José L. Siverio, Felipe De-León, Leandro Cubero, Jaime |
| author_browse | Alfaro-Fernández, Ana Asensio, Adrián Asensio-S.-Manzanera, Carmen Collar, Jesús Cubero, Jaime De-León, Leandro García-Méndez, Eva Garita-Cambronero, Jerson Marquínez, Raquel Martínez, M. Carmen Palomo, José L. Pérez-Padilla, Verónica Redondo, Cristina Ruiz-Padilla, Ana Siverio, Felipe |
| author_facet | Ruiz-Padilla, Ana Redondo, Cristina Asensio, Adrián Garita-Cambronero, Jerson Martínez, M. Carmen Pérez-Padilla, Verónica Marquínez, Raquel Collar, Jesús García-Méndez, Eva Alfaro-Fernández, Ana Asensio-S.-Manzanera, Carmen Palomo, José L. Siverio, Felipe De-León, Leandro Cubero, Jaime |
| author_sort | Ruiz-Padilla, Ana |
| collection | ReDivia |
| description | Liberibacter is a bacterial group causing different diseases and disorders in plants. Among liberibacters, Candidatus Liberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes according to single nucleotide polymorphisms in genes associated with ribosomal elements. In order to characterize more precisely isolates of CLso identified in potato in Spain, a Multilocus Sequence Analysis (MLSA) was applied. This methodology was validated by a complete analysis of ten housekeeping genes that showed an absence of positive selection and a nearly neutral mechanism for their evolution. Most of the analysis performed with single housekeeping genes, as well as MLSA, grouped together isolates of CLso detected in potato crops in Spain within the haplotype E, undistinguishable from those infecting carrots, parsnips or celery. Moreover, the information from these housekeeping genes was used to estimate the evolutionary divergence among the different CLso by using the concatenated sequences of the genes assayed. Data obtained on the divergence among CLso haplotypes support the hypothesis of evolutionary events connected with different hosts, in different geographic areas, and possibly associated with different vectors. Our results demonstrate the absence in Spain of CLso isolates molecularly classified as haplotypes A and B, traditionally considered causal agents of zebra chip in potato, as well as the uncertain possibility of the present haplotype to produce major disease outbreaks in potato that may depend on many factors that should be further evaluated in future works. |
| format | Artículo |
| id | ReDivia6654 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia66542025-04-25T14:47:33Z Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain Ruiz-Padilla, Ana Redondo, Cristina Asensio, Adrián Garita-Cambronero, Jerson Martínez, M. Carmen Pérez-Padilla, Verónica Marquínez, Raquel Collar, Jesús García-Méndez, Eva Alfaro-Fernández, Ana Asensio-S.-Manzanera, Carmen Palomo, José L. Siverio, Felipe De-León, Leandro Cubero, Jaime Liberibacter Zebra chip MLSA HLB H20 Plant diseases Potatoes Carrots Parsnips Celery Citrus Liberibacter is a bacterial group causing different diseases and disorders in plants. Among liberibacters, Candidatus Liberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes according to single nucleotide polymorphisms in genes associated with ribosomal elements. In order to characterize more precisely isolates of CLso identified in potato in Spain, a Multilocus Sequence Analysis (MLSA) was applied. This methodology was validated by a complete analysis of ten housekeeping genes that showed an absence of positive selection and a nearly neutral mechanism for their evolution. Most of the analysis performed with single housekeeping genes, as well as MLSA, grouped together isolates of CLso detected in potato crops in Spain within the haplotype E, undistinguishable from those infecting carrots, parsnips or celery. Moreover, the information from these housekeeping genes was used to estimate the evolutionary divergence among the different CLso by using the concatenated sequences of the genes assayed. Data obtained on the divergence among CLso haplotypes support the hypothesis of evolutionary events connected with different hosts, in different geographic areas, and possibly associated with different vectors. Our results demonstrate the absence in Spain of CLso isolates molecularly classified as haplotypes A and B, traditionally considered causal agents of zebra chip in potato, as well as the uncertain possibility of the present haplotype to produce major disease outbreaks in potato that may depend on many factors that should be further evaluated in future works. 2020-10-16T12:16:16Z 2020-10-16T12:16:16Z 2020 article publishedVersion Ruiz-Padilla, A., Redondo, C., Asensio, A., Garita-Cambronero, J., Martínez, C., Pérez-Padilla, V. et al.(2020). Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain. Microorganisms, 8(9), 1446. 2076-2607 http://hdl.handle.net/20.500.11939/6654 10.3390/microorganisms8091446 https://www.mdpi.com/2076-2607/8/9/1446 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Liberibacter Zebra chip MLSA HLB H20 Plant diseases Potatoes Carrots Parsnips Celery Citrus Ruiz-Padilla, Ana Redondo, Cristina Asensio, Adrián Garita-Cambronero, Jerson Martínez, M. Carmen Pérez-Padilla, Verónica Marquínez, Raquel Collar, Jesús García-Méndez, Eva Alfaro-Fernández, Ana Asensio-S.-Manzanera, Carmen Palomo, José L. Siverio, Felipe De-León, Leandro Cubero, Jaime Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title | Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title_full | Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title_fullStr | Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title_full_unstemmed | Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title_short | Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain |
| title_sort | assessment of multilocus sequence analysis mlsa for identification of candidatus liberibacter solanacearum from different host plants in spain |
| topic | Liberibacter Zebra chip MLSA HLB H20 Plant diseases Potatoes Carrots Parsnips Celery Citrus |
| url | http://hdl.handle.net/20.500.11939/6654 https://www.mdpi.com/2076-2607/8/9/1446 |
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