A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’
‘Concord’, the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special ‘foxy’ aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6653 https://www.nature.com/articles/s41438-020-0304-6 |
| _version_ | 1855492125893853184 |
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| author | Yang, Yingzhen Cuenca, José Wang, Nian Liang, Zhenchang Sun, Honghe Gutiérrez, Benjamín Xi, Xiaojun Arro, Jie Wang, Yi Fan, Peige Londo, Jason Cousins, Peter Li, Shaohua Fei, Zhangjun Zhong, Gan-Yuan |
| author_browse | Arro, Jie Cousins, Peter Cuenca, José Fan, Peige Fei, Zhangjun Gutiérrez, Benjamín Li, Shaohua Liang, Zhenchang Londo, Jason Sun, Honghe Wang, Nian Wang, Yi Xi, Xiaojun Yang, Yingzhen Zhong, Gan-Yuan |
| author_facet | Yang, Yingzhen Cuenca, José Wang, Nian Liang, Zhenchang Sun, Honghe Gutiérrez, Benjamín Xi, Xiaojun Arro, Jie Wang, Yi Fan, Peige Londo, Jason Cousins, Peter Li, Shaohua Fei, Zhangjun Zhong, Gan-Yuan |
| author_sort | Yang, Yingzhen |
| collection | ReDivia |
| description | ‘Concord’, the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special ‘foxy’ aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. vinifera. Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key ‘foxy’ aroma gene, anthraniloyl-CoA:methanol acyltransferase (AMAT), that is responsible for MA biosynthesis. We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in ‘Concord’ and other V. labrusca-derived grapes. These promoter variants, all with direct and inverted repeats, were further confirmed in more than 1,300 Vitis germplasm. Moreover, functional impact of these indels was validated in transgenic Arabidopsis. Superimposed on the promoter regulation, large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V. vinifera grapes. Elucidation of the AMAT genetic regulation advances our understanding of the ‘foxy’ aroma trait and makes it genetically trackable and amenable in grapevine breeding. |
| format | Artículo |
| id | ReDivia6653 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia66532025-04-25T14:47:33Z A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ Yang, Yingzhen Cuenca, José Wang, Nian Liang, Zhenchang Sun, Honghe Gutiérrez, Benjamín Xi, Xiaojun Arro, Jie Wang, Yi Fan, Peige Londo, Jason Cousins, Peter Li, Shaohua Fei, Zhangjun Zhong, Gan-Yuan F30 Plant genetics and breeding F70 Plant taxonomy and geography Q01 Food science and technology Grape juice Grapevines Vitis labrusca Arabidopsis ‘Concord’, the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special ‘foxy’ aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. vinifera. Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key ‘foxy’ aroma gene, anthraniloyl-CoA:methanol acyltransferase (AMAT), that is responsible for MA biosynthesis. We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in ‘Concord’ and other V. labrusca-derived grapes. These promoter variants, all with direct and inverted repeats, were further confirmed in more than 1,300 Vitis germplasm. Moreover, functional impact of these indels was validated in transgenic Arabidopsis. Superimposed on the promoter regulation, large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V. vinifera grapes. Elucidation of the AMAT genetic regulation advances our understanding of the ‘foxy’ aroma trait and makes it genetically trackable and amenable in grapevine breeding. 2020-10-16T12:14:16Z 2020-10-16T12:14:16Z 2020 article publishedVersion Yang, Y., Cuenca, J., Wang, N., Liang, Z., Sun, H., Gutierrez, B. et al. (2020). A key ‘foxy’aroma gene is regulated by homology-induced promoter indels in the iconic juice grape ‘Concord’. Horticulture Research, 7(1), 1-12. 2052-7276 (online) http://hdl.handle.net/20.500.11939/6653 10.1038/s41438-020-0304-6 https://www.nature.com/articles/s41438-020-0304-6 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer Nature electronico |
| spellingShingle | F30 Plant genetics and breeding F70 Plant taxonomy and geography Q01 Food science and technology Grape juice Grapevines Vitis labrusca Arabidopsis Yang, Yingzhen Cuenca, José Wang, Nian Liang, Zhenchang Sun, Honghe Gutiérrez, Benjamín Xi, Xiaojun Arro, Jie Wang, Yi Fan, Peige Londo, Jason Cousins, Peter Li, Shaohua Fei, Zhangjun Zhong, Gan-Yuan A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title | A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title_full | A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title_fullStr | A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title_full_unstemmed | A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title_short | A key ‘foxy’ aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape ‘Concord’ |
| title_sort | key foxy aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape concord |
| topic | F30 Plant genetics and breeding F70 Plant taxonomy and geography Q01 Food science and technology Grape juice Grapevines Vitis labrusca Arabidopsis |
| url | http://hdl.handle.net/20.500.11939/6653 https://www.nature.com/articles/s41438-020-0304-6 |
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