Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication
Cultivated melon was domesticated from wild melons, which produce small fruits with non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (paqt6.1) linked to fruit fles...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6651 https://www.mdpi.com/2073-4395/10/8/1064 |
| _version_ | 1855492125506928640 |
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| author | Riahi, Chaymaa Reig-Valiente, Juan L. Picó, Belén Díaz, Aurora Gonzalo, María J. Monforte, Antonio J. |
| author_browse | Díaz, Aurora Gonzalo, María J. Monforte, Antonio J. Picó, Belén Reig-Valiente, Juan L. Riahi, Chaymaa |
| author_facet | Riahi, Chaymaa Reig-Valiente, Juan L. Picó, Belén Díaz, Aurora Gonzalo, María J. Monforte, Antonio J. |
| author_sort | Riahi, Chaymaa |
| collection | ReDivia |
| description | Cultivated melon was domesticated from wild melons, which produce small fruits with
non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in
this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (paqt6.1) linked to
fruit flesh content was detected in a cross between cultivated (“Piel de Sapo”, PS) and wild (Ames
24294, TRI) accessions. The QTL was introgressed into the PS background, generating the TRI_6-3
introgression line (IL) that confirmed the e ects of paqt6.1. The primary objective of this work was to
fine-map paqt6.1 as the first step for the map-based cloning. Two di erent approaches were carried
out; however, the results were not consistent, precluding the fine mapping of paqt6.1. TRI_6-3 and
other related ILs were genotyped by genotyping-by-sequencing, finding additional introgressions
in other chromosomes. In an F2 population from TRI_6-3-x-PS, we found an epistatic interaction
between paqt6.1 and another locus on chromosome 11. The interaction was verified in advanced
populations, suggesting that the e ects of paqt6.1 are conditioned by the allelic composition at another
locus in chromosome 11. Both loci should have TRI alleles to reduce the flesh content in the PS
background. The implications on the history of melon domestication are discussed. |
| format | Artículo |
| id | ReDivia6651 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia66512025-04-25T14:47:32Z Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication Riahi, Chaymaa Reig-Valiente, Juan L. Picó, Belén Díaz, Aurora Gonzalo, María J. Monforte, Antonio J. QTL cloning Fine mapping F30 Plant genetics and breeding Cucumis melo Epistasis Pericarp Cultivated melon was domesticated from wild melons, which produce small fruits with non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (paqt6.1) linked to fruit flesh content was detected in a cross between cultivated (“Piel de Sapo”, PS) and wild (Ames 24294, TRI) accessions. The QTL was introgressed into the PS background, generating the TRI_6-3 introgression line (IL) that confirmed the e ects of paqt6.1. The primary objective of this work was to fine-map paqt6.1 as the first step for the map-based cloning. Two di erent approaches were carried out; however, the results were not consistent, precluding the fine mapping of paqt6.1. TRI_6-3 and other related ILs were genotyped by genotyping-by-sequencing, finding additional introgressions in other chromosomes. In an F2 population from TRI_6-3-x-PS, we found an epistatic interaction between paqt6.1 and another locus on chromosome 11. The interaction was verified in advanced populations, suggesting that the e ects of paqt6.1 are conditioned by the allelic composition at another locus in chromosome 11. Both loci should have TRI alleles to reduce the flesh content in the PS background. The implications on the history of melon domestication are discussed. 2020-10-14T08:55:32Z 2020-10-14T08:55:32Z 2020 article publishedVersion Riahi, C., Reig-Valiente, J. L., Picó, B., Díaz, A., Gonzalo, M. J., & Monforte, A. J. (2020). Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication. Agronomy, 10(8), 1064. 2073-4395 http://hdl.handle.net/20.500.11939/6651 10.3390/agronomy10081064 https://www.mdpi.com/2073-4395/10/8/1064 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | QTL cloning Fine mapping F30 Plant genetics and breeding Cucumis melo Epistasis Pericarp Riahi, Chaymaa Reig-Valiente, Juan L. Picó, Belén Díaz, Aurora Gonzalo, María J. Monforte, Antonio J. Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_full | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_fullStr | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_full_unstemmed | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_short | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_sort | evidence of the role of qtl epistatic interactions in the increase of melon fruit flesh content during domestication |
| topic | QTL cloning Fine mapping F30 Plant genetics and breeding Cucumis melo Epistasis Pericarp |
| url | http://hdl.handle.net/20.500.11939/6651 https://www.mdpi.com/2073-4395/10/8/1064 |
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