Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort

The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which depr...

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Autores principales: García-Domínguez, Ximo, Salvador Vicente, José, Viudes-De-Castro, María P., Marco-Jiménez, Francisco
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/6637
https://www.mdpi.com/2076-2615/10/8/1272
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author García-Domínguez, Ximo
Salvador Vicente, José
Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
author_browse García-Domínguez, Ximo
Marco-Jiménez, Francisco
Salvador Vicente, José
Viudes-De-Castro, María P.
author_facet García-Domínguez, Ximo
Salvador Vicente, José
Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
author_sort García-Domínguez, Ximo
collection ReDivia
description The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations was the principal trigger of transmissible effects, rather than embryo cryopreservation per se.
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spelling ReDivia66372025-04-25T14:47:29Z Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort García-Domínguez, Ximo Salvador Vicente, José Viudes-De-Castro, María P. Marco-Jiménez, Francisco Assisted reproduction technology Paternal inheritance Maternal inheritance Embryo cryopreservation Long-term effects Developmental plasticity Developmental programming L10 Animal genetics and breeding Embryo transfer The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations was the principal trigger of transmissible effects, rather than embryo cryopreservation per se. 2020-10-14T08:32:52Z 2020-10-14T08:32:52Z 2020 article publishedVersion Garcia-Dominguez, X., Vicente, J. S., Viudes-de-Castro, M. P., & Marco-Jiménez, F. (2020). Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort. Animals, 10(8), 1272. 2076-2615 http://hdl.handle.net/20.500.11939/6637 10.3390/ani10081272 https://www.mdpi.com/2076-2615/10/8/1272 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico
spellingShingle Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
L10 Animal genetics and breeding
Embryo transfer
García-Domínguez, Ximo
Salvador Vicente, José
Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_full Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_fullStr Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_full_unstemmed Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_short Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_sort long term effects following fresh vitrified embryo transfer are transmitted by paternal germline in a large size rabbit cohort
topic Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
L10 Animal genetics and breeding
Embryo transfer
url http://hdl.handle.net/20.500.11939/6637
https://www.mdpi.com/2076-2615/10/8/1272
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