RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing
Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite, single-stranded positive-sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in viral replication whereas RNA2 encodes two coat proteins (the large and sma...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
BSPP and John Wiley & Sons Ltd.
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6634 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12979 |
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| author | Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Medina, Vicente Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis |
| author_browse | Carpino, Caterina Davino, Salvatore Elvira-González, Laura Ferriol, Inmaculada Galipienso, Luis Medina, Vicente Peri, Ezio Rubio, Luis |
| author_facet | Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Medina, Vicente Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis |
| author_sort | Carpino, Caterina |
| collection | ReDivia |
| description | Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite,
single-stranded positive-sense RNA virus infecting many horticultural and ornamental
crops worldwide. RNA1 encodes proteins involved in viral replication whereas
RNA2 encodes two coat proteins (the large and small coat proteins) and two putative
movement proteins (MPs) of different sizes with overlapping C-terminal regions. In
this work, we determined the role played by the small putative BBWV-1 MP (VP37)
on virus pathogenicity, host specificity, and suppression of post-transcriptional gene
silencing (PTGS). We engineered a BBWV-1 35S-driven full-length cDNA infectious
clone corresponding to BBWV-1 RNA1 and RNA2 (pBBWV1-Wt) and generated a
mutant knocking out VP37 (pBBWV1-G492C). Agroinfiltration assays showed that
pBBWV1-Wt, as the original BBWV-1 isolate, infected broad bean, tomato, pepper,
and Nicotiana benthamiana, whereas pBBWV1-G492C did not infect pepper and tomato
systemically. Also, pBBWV1-G492C induced milder symptoms in broad bean
and N. benthamiana than pBBWV1-Wt. Differential retrotranscription and amplification
of the (+) and (−) strands showed that pBBWV1-G492C replicated in the agroinfiltrated
leaves of pepper but not in tomato. All this suggests that VP37 is a determinant
of pathogenicity and host specificity. Transient expression of VP37 through a potato
virus X (PVX) vector enhanced PVX symptoms and induced systemic necrosis associated
with programmed cell death in N. benthamiana plants. Finally, VP37 was identified
as a viral suppressor of RNA silencing by transient expression in N. benthamiana
16c plants and movement complementation of a viral construct based on turnip crinkle
virus (pTCV-GFP). |
| format | Artículo |
| id | ReDivia6634 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | BSPP and John Wiley & Sons Ltd. |
| publisherStr | BSPP and John Wiley & Sons Ltd. |
| record_format | dspace |
| spelling | ReDivia66342025-04-25T14:47:28Z RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Medina, Vicente Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis BBWV-1 Deteminant of Pathogenicity Fabavirus Infectious clone Secoviridae VSR H20 Plant diseases Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite, single-stranded positive-sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in viral replication whereas RNA2 encodes two coat proteins (the large and small coat proteins) and two putative movement proteins (MPs) of different sizes with overlapping C-terminal regions. In this work, we determined the role played by the small putative BBWV-1 MP (VP37) on virus pathogenicity, host specificity, and suppression of post-transcriptional gene silencing (PTGS). We engineered a BBWV-1 35S-driven full-length cDNA infectious clone corresponding to BBWV-1 RNA1 and RNA2 (pBBWV1-Wt) and generated a mutant knocking out VP37 (pBBWV1-G492C). Agroinfiltration assays showed that pBBWV1-Wt, as the original BBWV-1 isolate, infected broad bean, tomato, pepper, and Nicotiana benthamiana, whereas pBBWV1-G492C did not infect pepper and tomato systemically. Also, pBBWV1-G492C induced milder symptoms in broad bean and N. benthamiana than pBBWV1-Wt. Differential retrotranscription and amplification of the (+) and (−) strands showed that pBBWV1-G492C replicated in the agroinfiltrated leaves of pepper but not in tomato. All this suggests that VP37 is a determinant of pathogenicity and host specificity. Transient expression of VP37 through a potato virus X (PVX) vector enhanced PVX symptoms and induced systemic necrosis associated with programmed cell death in N. benthamiana plants. Finally, VP37 was identified as a viral suppressor of RNA silencing by transient expression in N. benthamiana 16c plants and movement complementation of a viral construct based on turnip crinkle virus (pTCV-GFP). 2020-10-14T08:24:57Z 2020-10-14T08:24:57Z 2020 article publishedVersion Carpino, C., Ferriol Safont, I., Elvira‐González, L., Medina, V., Rubio, L., Peri, E., ... & Galipienso Torregrosa, L. (2020). RNA2‐encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post‐transcriptional gene silencing. Molecular Plant Pathology, 21(11), 1421-1435. 1364-3703 http://hdl.handle.net/20.500.11939/6634 10.1111/mpp.12979 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12979 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess BSPP and John Wiley & Sons Ltd. electronico |
| spellingShingle | BBWV-1 Deteminant of Pathogenicity Fabavirus Infectious clone Secoviridae VSR H20 Plant diseases Carpino, Caterina Ferriol, Inmaculada Elvira-González, Laura Medina, Vicente Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title | RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title_full | RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title_fullStr | RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title_full_unstemmed | RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title_short | RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing |
| title_sort | rna2 encoded vp37 protein of broad bean wilt virus 1 is a determinant of pathogenicity host susceptibility and a suppressor of post transcriptional gene silencing |
| topic | BBWV-1 Deteminant of Pathogenicity Fabavirus Infectious clone Secoviridae VSR H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6634 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12979 |
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