Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus
Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarza...
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| Format: | publishedVersion |
| Language: | Inglés |
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MDPI
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6614 https://www.mdpi.com/2223-7747/9/10/1272 |
| _version_ | 1855032458792140800 |
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| author | Ruiz-García, Ana B. Candresse, Thierry Canales, Celia Morán, Félix Machado de Oliveira, Carlos Bertolini, Edson Olmos, Antonio |
| author_browse | Bertolini, Edson Canales, Celia Candresse, Thierry Machado de Oliveira, Carlos Morán, Félix Olmos, Antonio Ruiz-García, Ana B. |
| author_facet | Ruiz-García, Ana B. Candresse, Thierry Canales, Celia Morán, Félix Machado de Oliveira, Carlos Bertolini, Edson Olmos, Antonio |
| author_sort | Ruiz-García, Ana B. |
| collection | ReDivia |
| description | Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the Closteroviridae family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the Closteroviridae family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family. |
| format | publishedVersion |
| id | ReDivia6614 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia66142025-04-25T14:47:27Z Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus Ruiz-García, Ana B. Candresse, Thierry Canales, Celia Morán, Félix Machado de Oliveira, Carlos Bertolini, Edson Olmos, Antonio Closteroviridae thaumatin-like protein genome high-throughput sequencing NGS Olive leaf yellowing-associated virus H20 Plant diseases Olives Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the Closteroviridae family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the Closteroviridae family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family. 2020-09-30T08:10:40Z 2020-09-30T08:10:40Z 2020 publishedVersion Ruiz-García, A.B.; Candresse, T.; Canales, C.; Morán, F.; Machado de Oliveira, C.; Bertolini, E.; Olmos, A. (2020). Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus. Plants 2020, 9 (10), 1272. 2223-7747 http://hdl.handle.net/20.500.11939/6614 10.3390/plants9101272 https://www.mdpi.com/2223-7747/9/10/1272 en info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51912 IVIA 51912 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Closteroviridae thaumatin-like protein genome high-throughput sequencing NGS Olive leaf yellowing-associated virus H20 Plant diseases Olives Ruiz-García, Ana B. Candresse, Thierry Canales, Celia Morán, Félix Machado de Oliveira, Carlos Bertolini, Edson Olmos, Antonio Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title | Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title_full | Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title_fullStr | Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title_full_unstemmed | Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title_short | Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus |
| title_sort | molecular characterization of the complete coding sequence of olive leaf yellowing associated virus |
| topic | Closteroviridae thaumatin-like protein genome high-throughput sequencing NGS Olive leaf yellowing-associated virus H20 Plant diseases Olives |
| url | http://hdl.handle.net/20.500.11939/6614 https://www.mdpi.com/2223-7747/9/10/1272 |
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