Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings
Tetraploid citrus seedlings are more tolerant to salt stress than diploid genotypes. To provide insight into the causes of differences in salt tolerance due to ploidy and thus to better understand Cl− exclusion mechanisms in citrus, diploid and tetraploid seedlings of Carrizo citrange (CC) were grow...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6590 https://www.sciencedirect.com/science/article/abs/pii/S0176161716301614?via%3Dihub |
| _version_ | 1855492113766023168 |
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| author | Ruiz, Marta Quinones, Ana Martínez-Cuenca, Mary-Rus Aleza, Pablo Morillon, Raphael Navarro, Luis Primo-Millo, Eduardo Martínez-Alcántara, Belén |
| author_browse | Aleza, Pablo Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Morillon, Raphael Navarro, Luis Primo-Millo, Eduardo Quinones, Ana Ruiz, Marta |
| author_facet | Ruiz, Marta Quinones, Ana Martínez-Cuenca, Mary-Rus Aleza, Pablo Morillon, Raphael Navarro, Luis Primo-Millo, Eduardo Martínez-Alcántara, Belén |
| author_sort | Ruiz, Marta |
| collection | ReDivia |
| description | Tetraploid citrus seedlings are more tolerant to salt stress than diploid genotypes. To provide insight into the causes of differences in salt tolerance due to ploidy and thus to better understand Cl− exclusion mechanisms in citrus, diploid and tetraploid seedlings of Carrizo citrange (CC) were grown at 0 (control) and 40 mM NaCl (salt-treated) medium for 20 days. Chloride uptake and root-to-shoot translocation rates were on average 1.4-fold higher in diploid than in tetraploid salt-treated plants, which resulted in a greater (1.6-fold) Cl− build up in the leaves of the former. Root hydraulic conductance and leaf transpiration rate were 58% and 17% lower, respectively, in tetraploid than in diploid control plants. Differences remained after salt treatment which reduced these parameters by 30–40% in both genotypes. Morphology of the root system was significantly influenced by ploidy. Tetraploid roots were less branched and with lower number of root tips than those of diploid plants. The cross-section diameter and area were lower in the diploid, and consequently specific root length was higher (1.7-fold) than in tetraploid plants. The exodermis in sections close to the root apex was broader and with higher deposition of suberin in cell walls in the tetraploid than in the diploid genotype. Net CO2 assimilation rate in tetraploid salt-treated seedlings was 1.5-fold higher than in diploid salt-treated plants, likely due to the loss of photosynthetic capacity of diploid plants induced by Cl− toxicity. Leaf damage was much higher, in terms of burnt area and defoliation, in diploid than in tetraploid salt-treated plants (8- and 6-fold, respectively). Salt treatment significantly reduced (37%) the dry weight of the diploid plants, but did not affect the tetraploids. In conclusion, tetraploid CC plants appear more tolerant to salinization and this effect seems mainly due to differences in morphological and histological traits of roots affecting hydraulic conductance and transpiration rate. These results may suggest that tetraploid CC used as rootstock could improve salt tolerance in citrus trees. |
| format | Artículo |
| id | ReDivia6590 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia65902025-04-25T14:47:26Z Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings Ruiz, Marta Quinones, Ana Martínez-Cuenca, Mary-Rus Aleza, Pablo Morillon, Raphael Navarro, Luis Primo-Millo, Eduardo Martínez-Alcántara, Belén Cl transport Root anatomy F30 Plant genetics and breeding Polyploids Salt stress Suberin Tetraploid citrus seedlings are more tolerant to salt stress than diploid genotypes. To provide insight into the causes of differences in salt tolerance due to ploidy and thus to better understand Cl− exclusion mechanisms in citrus, diploid and tetraploid seedlings of Carrizo citrange (CC) were grown at 0 (control) and 40 mM NaCl (salt-treated) medium for 20 days. Chloride uptake and root-to-shoot translocation rates were on average 1.4-fold higher in diploid than in tetraploid salt-treated plants, which resulted in a greater (1.6-fold) Cl− build up in the leaves of the former. Root hydraulic conductance and leaf transpiration rate were 58% and 17% lower, respectively, in tetraploid than in diploid control plants. Differences remained after salt treatment which reduced these parameters by 30–40% in both genotypes. Morphology of the root system was significantly influenced by ploidy. Tetraploid roots were less branched and with lower number of root tips than those of diploid plants. The cross-section diameter and area were lower in the diploid, and consequently specific root length was higher (1.7-fold) than in tetraploid plants. The exodermis in sections close to the root apex was broader and with higher deposition of suberin in cell walls in the tetraploid than in the diploid genotype. Net CO2 assimilation rate in tetraploid salt-treated seedlings was 1.5-fold higher than in diploid salt-treated plants, likely due to the loss of photosynthetic capacity of diploid plants induced by Cl− toxicity. Leaf damage was much higher, in terms of burnt area and defoliation, in diploid than in tetraploid salt-treated plants (8- and 6-fold, respectively). Salt treatment significantly reduced (37%) the dry weight of the diploid plants, but did not affect the tetraploids. In conclusion, tetraploid CC plants appear more tolerant to salinization and this effect seems mainly due to differences in morphological and histological traits of roots affecting hydraulic conductance and transpiration rate. These results may suggest that tetraploid CC used as rootstock could improve salt tolerance in citrus trees. 2020-09-02T08:39:32Z 2020-09-02T08:39:32Z 2016 article publishedVersion Ruiz, M., Quiñones, A., Martínez-Cuenca, M. R., Aleza, P., Morillon, R., Navarro, L., ... & Martínez-Alcántara, B. (2016). Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings. Journal of Plant Physiology, 205, 1-10. 0176-1617 http://hdl.handle.net/20.500.11939/6590 10.1016/j.jplph.2016.08.002 https://www.sciencedirect.com/science/article/abs/pii/S0176161716301614?via%3Dihub en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico |
| spellingShingle | Cl transport Root anatomy F30 Plant genetics and breeding Polyploids Salt stress Suberin Ruiz, Marta Quinones, Ana Martínez-Cuenca, Mary-Rus Aleza, Pablo Morillon, Raphael Navarro, Luis Primo-Millo, Eduardo Martínez-Alcántara, Belén Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title | Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title_full | Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title_fullStr | Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title_full_unstemmed | Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title_short | Tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| title_sort | tetraploidy enhances the ability to exclude chloride from leaves in carrizo citrange seedlings |
| topic | Cl transport Root anatomy F30 Plant genetics and breeding Polyploids Salt stress Suberin |
| url | http://hdl.handle.net/20.500.11939/6590 https://www.sciencedirect.com/science/article/abs/pii/S0176161716301614?via%3Dihub |
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