Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis

Iron (Fe) chlorosis is one of the commonest problems in calcareous soils and plant tolerance is determined mainly by rootstocks. This work evaluates new citrus hybrids’ tolerance to Fe chlorosis compared with the most widely used rootstocks in Spain: Citrus macrophylla (CM, Fe chlorosis-tolerant) an...

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Main Authors: Martínez-Cuenca, Mary-Rus, Quinones, Ana, Forner-Giner, María A.
Format: article
Language:Inglés
Published: Elsevier 2020
Subjects:
Online Access:http://hdl.handle.net/20.500.11939/6588
https://www.sciencedirect.com/science/article/abs/pii/S0304423815302673
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author Martínez-Cuenca, Mary-Rus
Quinones, Ana
Forner-Giner, María A.
author_browse Forner-Giner, María A.
Martínez-Cuenca, Mary-Rus
Quinones, Ana
author_facet Martínez-Cuenca, Mary-Rus
Quinones, Ana
Forner-Giner, María A.
author_sort Martínez-Cuenca, Mary-Rus
collection ReDivia
description Iron (Fe) chlorosis is one of the commonest problems in calcareous soils and plant tolerance is determined mainly by rootstocks. This work evaluates new citrus hybrids’ tolerance to Fe chlorosis compared with the most widely used rootstocks in Spain: Citrus macrophylla (CM, Fe chlorosis-tolerant) and Carrizo citrange (CC, Fe chlorosis-sensitive). Growth parameters, Fe concentration, photosynthetic parameters, ferric chelate reductase (FC-R) activity and proton (H+) extrusion capacity were assessed in plants irrigated with 20 (control, Ct) or 0 (Fe-deficient, -Fe) μM FeEDDHA. Some—Fe hybrids presented marked Fe chlorosis symptomatology reflected by the root:shoot ratio and Chl a and b concentrations sharply dropping, and an increase in the Chl a/b ratio. These effects were very strong in the 050119, 050124-B and 050110—Fe species. The net CO2 assimilation rate significantly lowered in the 05019, 050131, 050125, 050112 and 05013—Fe species. The 050120, 050125, 050112 and 050124-B—Fe plants presented lower stomatal conductance than the Ct ones. Conversely, the internal CO2 concentration (Ci) tended to increase in Fe-deprived plants. Fe-deficiency increased FC-R activity and H+ extrusion in some 0501 species. Both responses were significantly induced in the CM, 050114, 05019, 050131 and 050125—Fe plants. Fe2+ accumulation in—Fe plants related inversely with FC-R activity. Most—Fe species with the greatest FC-R activity also accumulated the most Fe3+ ions and, therefore, the Fe pool was much larger in the root apoplast. Collectively, the main trait that determined Fe-chlorosis tolerance among these genotypes was the ability to: (1) boost Fe3+ reduction in response to Fe-deficiency; (2) acidify root media; (3) benefit the Fe amounts stored in the root apoplast.
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spelling ReDivia65882025-04-25T14:47:25Z Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis Martínez-Cuenca, Mary-Rus Quinones, Ana Forner-Giner, María A. Chlorophyll concentration Citrus hybrids Fe2+ concentration Ferric-Chelate reductase Gas exchange parameters H+ extrusion Iron chlorosis P33 Soil chemistry and physics Iron (Fe) chlorosis is one of the commonest problems in calcareous soils and plant tolerance is determined mainly by rootstocks. This work evaluates new citrus hybrids’ tolerance to Fe chlorosis compared with the most widely used rootstocks in Spain: Citrus macrophylla (CM, Fe chlorosis-tolerant) and Carrizo citrange (CC, Fe chlorosis-sensitive). Growth parameters, Fe concentration, photosynthetic parameters, ferric chelate reductase (FC-R) activity and proton (H+) extrusion capacity were assessed in plants irrigated with 20 (control, Ct) or 0 (Fe-deficient, -Fe) μM FeEDDHA. Some—Fe hybrids presented marked Fe chlorosis symptomatology reflected by the root:shoot ratio and Chl a and b concentrations sharply dropping, and an increase in the Chl a/b ratio. These effects were very strong in the 050119, 050124-B and 050110—Fe species. The net CO2 assimilation rate significantly lowered in the 05019, 050131, 050125, 050112 and 05013—Fe species. The 050120, 050125, 050112 and 050124-B—Fe plants presented lower stomatal conductance than the Ct ones. Conversely, the internal CO2 concentration (Ci) tended to increase in Fe-deprived plants. Fe-deficiency increased FC-R activity and H+ extrusion in some 0501 species. Both responses were significantly induced in the CM, 050114, 05019, 050131 and 050125—Fe plants. Fe2+ accumulation in—Fe plants related inversely with FC-R activity. Most—Fe species with the greatest FC-R activity also accumulated the most Fe3+ ions and, therefore, the Fe pool was much larger in the root apoplast. Collectively, the main trait that determined Fe-chlorosis tolerance among these genotypes was the ability to: (1) boost Fe3+ reduction in response to Fe-deficiency; (2) acidify root media; (3) benefit the Fe amounts stored in the root apoplast. 2020-09-02T08:38:05Z 2020-09-02T08:38:05Z 2016 article publishedVersion Martínez-Cuenca, M. R., Quinones, A., & Forner-Giner, M. Á. (2016). Screening of ‘King’mandarin (Citrus nobilis Lour)× Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis. Scientia Horticulturae, 198, 61-69. 0304-4238 http://hdl.handle.net/20.500.11939/6588 10.1016/j.scienta.2015.10.038 https://www.sciencedirect.com/science/article/abs/pii/S0304423815302673 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ closedAccess Elsevier electronico
spellingShingle Chlorophyll concentration
Citrus hybrids
Fe2+ concentration
Ferric-Chelate reductase
Gas exchange parameters
H+ extrusion
Iron chlorosis
P33 Soil chemistry and physics
Martínez-Cuenca, Mary-Rus
Quinones, Ana
Forner-Giner, María A.
Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title_full Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title_fullStr Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title_full_unstemmed Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title_short Screening of ‘King’ mandarin (Citrus nobilis Lour) × Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis
title_sort screening of king mandarin citrus nobilis lour poncirus trifoliata l raf hybrids as citrus rootstocks tolerants to iron chlorosis
topic Chlorophyll concentration
Citrus hybrids
Fe2+ concentration
Ferric-Chelate reductase
Gas exchange parameters
H+ extrusion
Iron chlorosis
P33 Soil chemistry and physics
url http://hdl.handle.net/20.500.11939/6588
https://www.sciencedirect.com/science/article/abs/pii/S0304423815302673
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