Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain
Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of fou...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6566 https://www.mdpi.com/2073-4395/10/8/1062 |
| _version_ | 1855492109200523264 |
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| author | León, Maela Berbegal, Mónica Rodríguez-Reina, José M. Elena, Georgina Abad-Campos, Paloma Ramón-Albalat, Antonio Olmo, Diego Vicent, Antonio Luque, Jordi Miarnau, Xavier Agustí-Brisach, Carlos Trapero, Antonio Capote, Nieves Arroyo, Francisco T. Avilés, Manuel Gramaje, David Andrés-Sodupe, Marcos Armengol, Josep |
| author_browse | Abad-Campos, Paloma Agustí-Brisach, Carlos Andrés-Sodupe, Marcos Armengol, Josep Arroyo, Francisco T. Avilés, Manuel Berbegal, Mónica Capote, Nieves Elena, Georgina Gramaje, David León, Maela Luque, Jordi Miarnau, Xavier Olmo, Diego Ramón-Albalat, Antonio Rodríguez-Reina, José M. Trapero, Antonio Vicent, Antonio |
| author_facet | León, Maela Berbegal, Mónica Rodríguez-Reina, José M. Elena, Georgina Abad-Campos, Paloma Ramón-Albalat, Antonio Olmo, Diego Vicent, Antonio Luque, Jordi Miarnau, Xavier Agustí-Brisach, Carlos Trapero, Antonio Capote, Nieves Arroyo, Francisco T. Avilés, Manuel Gramaje, David Andrés-Sodupe, Marcos Armengol, Josep |
| author_sort | León, Maela |
| collection | ReDivia |
| description | Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop. |
| format | Artículo |
| id | ReDivia6566 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia65662025-04-25T14:47:23Z Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain León, Maela Berbegal, Mónica Rodríguez-Reina, José M. Elena, Georgina Abad-Campos, Paloma Ramón-Albalat, Antonio Olmo, Diego Vicent, Antonio Luque, Jordi Miarnau, Xavier Agustí-Brisach, Carlos Trapero, Antonio Capote, Nieves Arroyo, Francisco T. Avilés, Manuel Gramaje, David Andrés-Sodupe, Marcos Armengol, Josep Diaporthe amygdali D. mediterranea Multilocus DNA sequence analysis H20 Plant diseases Pathogenicity Prunus dulcis Diaporthe Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop. 2020-08-04T09:38:23Z 2020-08-04T09:38:23Z 2020 article publishedVersion León, M., Berbegal, M., Rodríguez-Reina, J. M., Elena, G., Abad-Campos, P., Ramón-Albalat, A., ... & Agustí-Brisach, C. (2020). Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain. Agronomy, 10(8), 1062. 2073-4395 http://hdl.handle.net/20.500.11939/6566 10.3390/agronomy10081062 https://www.mdpi.com/2073-4395/10/8/1062 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Diaporthe amygdali D. mediterranea Multilocus DNA sequence analysis H20 Plant diseases Pathogenicity Prunus dulcis Diaporthe León, Maela Berbegal, Mónica Rodríguez-Reina, José M. Elena, Georgina Abad-Campos, Paloma Ramón-Albalat, Antonio Olmo, Diego Vicent, Antonio Luque, Jordi Miarnau, Xavier Agustí-Brisach, Carlos Trapero, Antonio Capote, Nieves Arroyo, Francisco T. Avilés, Manuel Gramaje, David Andrés-Sodupe, Marcos Armengol, Josep Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title | Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title_full | Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title_fullStr | Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title_full_unstemmed | Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title_short | Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain |
| title_sort | identification and characterization of diaporthe spp associated with twig cankers and shoot blight of almonds in spain |
| topic | Diaporthe amygdali D. mediterranea Multilocus DNA sequence analysis H20 Plant diseases Pathogenicity Prunus dulcis Diaporthe |
| url | http://hdl.handle.net/20.500.11939/6566 https://www.mdpi.com/2073-4395/10/8/1062 |
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