Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045
During grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, and flavored berries. There is evidence that ethylene, together with other signals, plays an important role in triggering the onset of ripening. Here, we report the functional charac...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | article |
| Language: | Inglés |
| Published: |
Frontiers Media
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6540 https://www.frontiersin.org/articles/10.3389/fpls.2016.01793/full |
| _version_ | 1855032445195255808 |
|---|---|
| author | Leida, Carmen Dal Ri, Antonio Dalla Costa, Lorenza Gómez, María D. Pompili, Valerio Sonego, Paolo Engelen, Kristof Masuero, Domenico Ríos, Gabino Moser, Claudio |
| author_browse | Dal Ri, Antonio Dalla Costa, Lorenza Engelen, Kristof Gómez, María D. Leida, Carmen Masuero, Domenico Moser, Claudio Pompili, Valerio Ríos, Gabino Sonego, Paolo |
| author_facet | Leida, Carmen Dal Ri, Antonio Dalla Costa, Lorenza Gómez, María D. Pompili, Valerio Sonego, Paolo Engelen, Kristof Masuero, Domenico Ríos, Gabino Moser, Claudio |
| author_sort | Leida, Carmen |
| collection | ReDivia |
| description | During grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, and flavored berries. There is evidence that ethylene, together with other signals, plays an important role in triggering the onset of ripening. Here, we report the functional characterization of a berry-specific Ethylene Responsive Factor (ERF), VviERF045, which is induced just before véraison and peaks at ripening. Phylogenetic analysis revealed it is close to the SHINE clade of ERFs, factors involved in the regulation of wax biosynthesis and cuticle morphology. Transgenic grapevines lines overexpressing VviERF045 were obtained, in vitro propagated, phenotypically characterized, and analyzed for the content of specific classes of metabolites. The effect of VviERF045 was correlated with the level of transgene expression, with high-expressing lines showing stunted growth, discolored and smaller leaves, and a lower level of chlorophylls and carotenoids. One line with intermediate expression, L15, was characterized at the transcriptomic level and showed 573 differentially expressed genes compared to wild type plants. Microscopy and gene expression analyses point toward a major role of VviERF045 in epidermis patterning by acting on waxes and cuticle. They also indicate that VviERF045 affects phenolic secondary metabolism and induces a reaction resembling a plant immune response with modulation of receptor like-kinases and pathogen related genes. These results suggest also a possible role of this transcription factor in berry ripening, likely related to changes in epidermis and cuticle of the berry, cell expansion, a decrease in photosynthetic capacity, and the activation of several defense related genes as well as from the phenylpropanoid metabolism. All these processes occur in the berry during ripening. |
| format | article |
| id | ReDivia6540 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia65402025-04-25T14:47:21Z Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 Leida, Carmen Dal Ri, Antonio Dalla Costa, Lorenza Gómez, María D. Pompili, Valerio Sonego, Paolo Engelen, Kristof Masuero, Domenico Ríos, Gabino Moser, Claudio ERF (Ethylene Responsive Factor) over-expressing transgenic lines VOCs Wax J10 Handling, transport, storage and protection of agricultural products RNA sequence Vitis vinifera During grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, and flavored berries. There is evidence that ethylene, together with other signals, plays an important role in triggering the onset of ripening. Here, we report the functional characterization of a berry-specific Ethylene Responsive Factor (ERF), VviERF045, which is induced just before véraison and peaks at ripening. Phylogenetic analysis revealed it is close to the SHINE clade of ERFs, factors involved in the regulation of wax biosynthesis and cuticle morphology. Transgenic grapevines lines overexpressing VviERF045 were obtained, in vitro propagated, phenotypically characterized, and analyzed for the content of specific classes of metabolites. The effect of VviERF045 was correlated with the level of transgene expression, with high-expressing lines showing stunted growth, discolored and smaller leaves, and a lower level of chlorophylls and carotenoids. One line with intermediate expression, L15, was characterized at the transcriptomic level and showed 573 differentially expressed genes compared to wild type plants. Microscopy and gene expression analyses point toward a major role of VviERF045 in epidermis patterning by acting on waxes and cuticle. They also indicate that VviERF045 affects phenolic secondary metabolism and induces a reaction resembling a plant immune response with modulation of receptor like-kinases and pathogen related genes. These results suggest also a possible role of this transcription factor in berry ripening, likely related to changes in epidermis and cuticle of the berry, cell expansion, a decrease in photosynthetic capacity, and the activation of several defense related genes as well as from the phenylpropanoid metabolism. All these processes occur in the berry during ripening. 2020-06-18T08:03:24Z 2020-06-18T08:03:24Z 2016 article publishedVersion Leida, C., Dal Rì, A., Dalla Costa, L., Gómez, M. D., Pompili, V., Sonego, P., ... & Moser, C. (2016). Insights into the role of the berry-specific ethylene responsive factor VviERF045. Frontiers in plant science, 7, 1793. 1664-462X http://hdl.handle.net/20.500.11939/6540 10.3389/fpls.2016.01793 https://www.frontiersin.org/articles/10.3389/fpls.2016.01793/full en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Frontiers Media electronico |
| spellingShingle | ERF (Ethylene Responsive Factor) over-expressing transgenic lines VOCs Wax J10 Handling, transport, storage and protection of agricultural products RNA sequence Vitis vinifera Leida, Carmen Dal Ri, Antonio Dalla Costa, Lorenza Gómez, María D. Pompili, Valerio Sonego, Paolo Engelen, Kristof Masuero, Domenico Ríos, Gabino Moser, Claudio Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title | Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title_full | Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title_fullStr | Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title_full_unstemmed | Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title_short | Insights into the Role of the Berry-Specific Ethylene Responsive Factor VviERF045 |
| title_sort | insights into the role of the berry specific ethylene responsive factor vvierf045 |
| topic | ERF (Ethylene Responsive Factor) over-expressing transgenic lines VOCs Wax J10 Handling, transport, storage and protection of agricultural products RNA sequence Vitis vinifera |
| url | http://hdl.handle.net/20.500.11939/6540 https://www.frontiersin.org/articles/10.3389/fpls.2016.01793/full |
| work_keys_str_mv | AT leidacarmen insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT dalriantonio insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT dallacostalorenza insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT gomezmariad insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT pompilivalerio insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT sonegopaolo insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT engelenkristof insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT masuerodomenico insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT riosgabino insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 AT moserclaudio insightsintotheroleoftheberryspecificethyleneresponsivefactorvvierf045 |