Application of Genomic Technologies to the Breeding of Trees
The recent introduction of next generation sequencing (NGS) technologies represents a major revolution in providing new tools for identifying the genes and/or genomic intervals controlling important traits for selection in breeding programs. In perennial fruit trees with long generation times and l...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Frontiers Media
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6539 https://www.frontiersin.org/articles/10.3389/fgene.2016.00198/full |
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| author | Badenes, María L. Fernández i Martí, Ángel Ríos, Gabino Rubio-Cabetas, María J. |
| author_browse | Badenes, María L. Fernández i Martí, Ángel Rubio-Cabetas, María J. Ríos, Gabino |
| author_facet | Badenes, María L. Fernández i Martí, Ángel Ríos, Gabino Rubio-Cabetas, María J. |
| author_sort | Badenes, María L. |
| collection | ReDivia |
| description | The recent introduction of next generation sequencing (NGS) technologies represents a
major revolution in providing new tools for identifying the genes and/or genomic intervals controlling important traits for selection in breeding programs. In perennial fruit trees with long generation times and large sizes of adult plants, the impact of these techniques is even more important. High-throughput DNA sequencing technologies have provided complete annotated sequences in many important tree species. Most of the highthroughput genotyping platforms described are being used for studies of genetic diversity and population structure. Dissection of complex traits became possible through the availability of genome sequences along with phenotypic variation data, which allow to elucidate the causative genetic differences that give rise to observed phenotypic variation. Association mapping facilitates the association between genetic markers and phenotype in unstructured and complex populations, identifying molecular markers for assisted selection and breeding. Also, genomic data provide in silico identification and characterization of genes and gene families related to important traits, enabling new tools for molecular marker assisted selection in tree breeding. Deep sequencing of transcriptomes is also a powerful tool for the analysis of precise expression levels of each gene in a sample. It consists in quantifying short cDNA reads, obtained by NGS technologies, in order to compare the entire transcriptomes between genotypes and environmental conditions. The miRNAs are non-coding short RNAs involved in the regulation of different physiological processes, which can be identified by highthroughput sequencing of RNA libraries obtained by reverse transcription of purified
short RNAs, and by in silico comparison with known miRNAs from other species. All together, NGS techniques and their applications have increased the resources for plant breeding in tree species, closing the former gap of genetic tools between trees and
annual species. |
| format | article |
| id | ReDivia6539 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia65392025-04-25T14:47:20Z Application of Genomic Technologies to the Breeding of Trees Badenes, María L. Fernández i Martí, Ángel Ríos, Gabino Rubio-Cabetas, María J. Next generation sequencing Genome sequences Association mapping Insertional mutant populations F30 Plant genetics and breeding RNA sequence The recent introduction of next generation sequencing (NGS) technologies represents a major revolution in providing new tools for identifying the genes and/or genomic intervals controlling important traits for selection in breeding programs. In perennial fruit trees with long generation times and large sizes of adult plants, the impact of these techniques is even more important. High-throughput DNA sequencing technologies have provided complete annotated sequences in many important tree species. Most of the highthroughput genotyping platforms described are being used for studies of genetic diversity and population structure. Dissection of complex traits became possible through the availability of genome sequences along with phenotypic variation data, which allow to elucidate the causative genetic differences that give rise to observed phenotypic variation. Association mapping facilitates the association between genetic markers and phenotype in unstructured and complex populations, identifying molecular markers for assisted selection and breeding. Also, genomic data provide in silico identification and characterization of genes and gene families related to important traits, enabling new tools for molecular marker assisted selection in tree breeding. Deep sequencing of transcriptomes is also a powerful tool for the analysis of precise expression levels of each gene in a sample. It consists in quantifying short cDNA reads, obtained by NGS technologies, in order to compare the entire transcriptomes between genotypes and environmental conditions. The miRNAs are non-coding short RNAs involved in the regulation of different physiological processes, which can be identified by highthroughput sequencing of RNA libraries obtained by reverse transcription of purified short RNAs, and by in silico comparison with known miRNAs from other species. All together, NGS techniques and their applications have increased the resources for plant breeding in tree species, closing the former gap of genetic tools between trees and annual species. 2020-06-18T08:02:21Z 2020-06-18T08:02:21Z 2016 article publishedVersion Badenes, M. L., Fernandez i Marti, A., Ríos, G. & Rubio-Cabetas, M. J. (2016). Application of genomic technologies to the breeding of trees. Frontiers in genetics, 7, 198. 1664-8021 http://hdl.handle.net/20.500.11939/6539 10.3389/fgene.2016.00198 https://www.frontiersin.org/articles/10.3389/fgene.2016.00198/full en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Frontiers Media electronico |
| spellingShingle | Next generation sequencing Genome sequences Association mapping Insertional mutant populations F30 Plant genetics and breeding RNA sequence Badenes, María L. Fernández i Martí, Ángel Ríos, Gabino Rubio-Cabetas, María J. Application of Genomic Technologies to the Breeding of Trees |
| title | Application of Genomic Technologies to the Breeding of Trees |
| title_full | Application of Genomic Technologies to the Breeding of Trees |
| title_fullStr | Application of Genomic Technologies to the Breeding of Trees |
| title_full_unstemmed | Application of Genomic Technologies to the Breeding of Trees |
| title_short | Application of Genomic Technologies to the Breeding of Trees |
| title_sort | application of genomic technologies to the breeding of trees |
| topic | Next generation sequencing Genome sequences Association mapping Insertional mutant populations F30 Plant genetics and breeding RNA sequence |
| url | http://hdl.handle.net/20.500.11939/6539 https://www.frontiersin.org/articles/10.3389/fgene.2016.00198/full |
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